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Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes

Neural progenitor cell (NPC) transplantation is a promising approach for repairing spinal cord injury (SCI). However, cell survival, maturation and integration after transplantation are still major challenges. Here, we produced a novel centimeter‐scale human spinal cord neural tissue (hscNT) constru...

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Autores principales: Jin, Chen, Wu, Yayu, Zhang, Haipeng, Xu, Bai, Liu, Wenbin, Ji, Chunnan, Li, Panpan, Chen, Zhenni, Chen, Bing, Li, Jiayin, Wu, Xianming, Jiang, Peipei, Hu, Yali, Xiao, Zhifeng, Zhao, Yannan, Dai, Jianwu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013752/
https://www.ncbi.nlm.nih.gov/pubmed/36925694
http://dx.doi.org/10.1002/btm2.10448
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author Jin, Chen
Wu, Yayu
Zhang, Haipeng
Xu, Bai
Liu, Wenbin
Ji, Chunnan
Li, Panpan
Chen, Zhenni
Chen, Bing
Li, Jiayin
Wu, Xianming
Jiang, Peipei
Hu, Yali
Xiao, Zhifeng
Zhao, Yannan
Dai, Jianwu
author_facet Jin, Chen
Wu, Yayu
Zhang, Haipeng
Xu, Bai
Liu, Wenbin
Ji, Chunnan
Li, Panpan
Chen, Zhenni
Chen, Bing
Li, Jiayin
Wu, Xianming
Jiang, Peipei
Hu, Yali
Xiao, Zhifeng
Zhao, Yannan
Dai, Jianwu
author_sort Jin, Chen
collection PubMed
description Neural progenitor cell (NPC) transplantation is a promising approach for repairing spinal cord injury (SCI). However, cell survival, maturation and integration after transplantation are still major challenges. Here, we produced a novel centimeter‐scale human spinal cord neural tissue (hscNT) construct with human spinal cord neural progenitor cells (hscNPCs) and human spinal cord astrocytes (hscAS) on a linearly ordered collagen scaffold (LOCS). The hscAS promoted hscNPC adhesion, survival and neurite outgrowth on the LOCS, to form a linearly ordered spinal cord‐like structure consisting of mature neurons and glia cells. When transplanted into rats with SCI, the hscNT created a favorable microenvironment by inhibiting inflammation and glial scar formation, and promoted neural and vascular regeneration. Notably, the hscNT promoted neural circuit reconstruction and motor functional recovery. Engineered human spinal cord implants containing astrocytes and neurons assembled on axon guidance scaffolds may therefore have potential in the treatment of SCI.
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spelling pubmed-100137522023-03-15 Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes Jin, Chen Wu, Yayu Zhang, Haipeng Xu, Bai Liu, Wenbin Ji, Chunnan Li, Panpan Chen, Zhenni Chen, Bing Li, Jiayin Wu, Xianming Jiang, Peipei Hu, Yali Xiao, Zhifeng Zhao, Yannan Dai, Jianwu Bioeng Transl Med Research Articles Neural progenitor cell (NPC) transplantation is a promising approach for repairing spinal cord injury (SCI). However, cell survival, maturation and integration after transplantation are still major challenges. Here, we produced a novel centimeter‐scale human spinal cord neural tissue (hscNT) construct with human spinal cord neural progenitor cells (hscNPCs) and human spinal cord astrocytes (hscAS) on a linearly ordered collagen scaffold (LOCS). The hscAS promoted hscNPC adhesion, survival and neurite outgrowth on the LOCS, to form a linearly ordered spinal cord‐like structure consisting of mature neurons and glia cells. When transplanted into rats with SCI, the hscNT created a favorable microenvironment by inhibiting inflammation and glial scar formation, and promoted neural and vascular regeneration. Notably, the hscNT promoted neural circuit reconstruction and motor functional recovery. Engineered human spinal cord implants containing astrocytes and neurons assembled on axon guidance scaffolds may therefore have potential in the treatment of SCI. John Wiley & Sons, Inc. 2022-11-09 /pmc/articles/PMC10013752/ /pubmed/36925694 http://dx.doi.org/10.1002/btm2.10448 Text en © 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Jin, Chen
Wu, Yayu
Zhang, Haipeng
Xu, Bai
Liu, Wenbin
Ji, Chunnan
Li, Panpan
Chen, Zhenni
Chen, Bing
Li, Jiayin
Wu, Xianming
Jiang, Peipei
Hu, Yali
Xiao, Zhifeng
Zhao, Yannan
Dai, Jianwu
Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes
title Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes
title_full Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes
title_fullStr Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes
title_full_unstemmed Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes
title_short Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes
title_sort spinal cord tissue engineering using human primary neural progenitor cells and astrocytes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013752/
https://www.ncbi.nlm.nih.gov/pubmed/36925694
http://dx.doi.org/10.1002/btm2.10448
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