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Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes
Neural progenitor cell (NPC) transplantation is a promising approach for repairing spinal cord injury (SCI). However, cell survival, maturation and integration after transplantation are still major challenges. Here, we produced a novel centimeter‐scale human spinal cord neural tissue (hscNT) constru...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013752/ https://www.ncbi.nlm.nih.gov/pubmed/36925694 http://dx.doi.org/10.1002/btm2.10448 |
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author | Jin, Chen Wu, Yayu Zhang, Haipeng Xu, Bai Liu, Wenbin Ji, Chunnan Li, Panpan Chen, Zhenni Chen, Bing Li, Jiayin Wu, Xianming Jiang, Peipei Hu, Yali Xiao, Zhifeng Zhao, Yannan Dai, Jianwu |
author_facet | Jin, Chen Wu, Yayu Zhang, Haipeng Xu, Bai Liu, Wenbin Ji, Chunnan Li, Panpan Chen, Zhenni Chen, Bing Li, Jiayin Wu, Xianming Jiang, Peipei Hu, Yali Xiao, Zhifeng Zhao, Yannan Dai, Jianwu |
author_sort | Jin, Chen |
collection | PubMed |
description | Neural progenitor cell (NPC) transplantation is a promising approach for repairing spinal cord injury (SCI). However, cell survival, maturation and integration after transplantation are still major challenges. Here, we produced a novel centimeter‐scale human spinal cord neural tissue (hscNT) construct with human spinal cord neural progenitor cells (hscNPCs) and human spinal cord astrocytes (hscAS) on a linearly ordered collagen scaffold (LOCS). The hscAS promoted hscNPC adhesion, survival and neurite outgrowth on the LOCS, to form a linearly ordered spinal cord‐like structure consisting of mature neurons and glia cells. When transplanted into rats with SCI, the hscNT created a favorable microenvironment by inhibiting inflammation and glial scar formation, and promoted neural and vascular regeneration. Notably, the hscNT promoted neural circuit reconstruction and motor functional recovery. Engineered human spinal cord implants containing astrocytes and neurons assembled on axon guidance scaffolds may therefore have potential in the treatment of SCI. |
format | Online Article Text |
id | pubmed-10013752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100137522023-03-15 Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes Jin, Chen Wu, Yayu Zhang, Haipeng Xu, Bai Liu, Wenbin Ji, Chunnan Li, Panpan Chen, Zhenni Chen, Bing Li, Jiayin Wu, Xianming Jiang, Peipei Hu, Yali Xiao, Zhifeng Zhao, Yannan Dai, Jianwu Bioeng Transl Med Research Articles Neural progenitor cell (NPC) transplantation is a promising approach for repairing spinal cord injury (SCI). However, cell survival, maturation and integration after transplantation are still major challenges. Here, we produced a novel centimeter‐scale human spinal cord neural tissue (hscNT) construct with human spinal cord neural progenitor cells (hscNPCs) and human spinal cord astrocytes (hscAS) on a linearly ordered collagen scaffold (LOCS). The hscAS promoted hscNPC adhesion, survival and neurite outgrowth on the LOCS, to form a linearly ordered spinal cord‐like structure consisting of mature neurons and glia cells. When transplanted into rats with SCI, the hscNT created a favorable microenvironment by inhibiting inflammation and glial scar formation, and promoted neural and vascular regeneration. Notably, the hscNT promoted neural circuit reconstruction and motor functional recovery. Engineered human spinal cord implants containing astrocytes and neurons assembled on axon guidance scaffolds may therefore have potential in the treatment of SCI. John Wiley & Sons, Inc. 2022-11-09 /pmc/articles/PMC10013752/ /pubmed/36925694 http://dx.doi.org/10.1002/btm2.10448 Text en © 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Jin, Chen Wu, Yayu Zhang, Haipeng Xu, Bai Liu, Wenbin Ji, Chunnan Li, Panpan Chen, Zhenni Chen, Bing Li, Jiayin Wu, Xianming Jiang, Peipei Hu, Yali Xiao, Zhifeng Zhao, Yannan Dai, Jianwu Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes |
title | Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes |
title_full | Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes |
title_fullStr | Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes |
title_full_unstemmed | Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes |
title_short | Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes |
title_sort | spinal cord tissue engineering using human primary neural progenitor cells and astrocytes |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013752/ https://www.ncbi.nlm.nih.gov/pubmed/36925694 http://dx.doi.org/10.1002/btm2.10448 |
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