Cargando…

Fluidic integrated 3D bioprinting system to sustain cell viability towards larynx fabrication

Herein, we report the first study to create a three‐dimensional (3D) bioprinted artificial larynx for whole‐laryngeal replacement. Our 3D bio‐printed larynx was generated using extrusion‐based 3D bioprinter with rabbit's chondrocyte‐laden gelatin methacryloyl (GelMA)/glycidyl‐methacrylated hyal...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, Hae Sang, Lee, Ji Seung, Kim, Chang‐Beom, Lee, Kwang‐Ho, Hong, In‐Sun, Jung, Harry, Lee, Hanna, Lee, Young Jin, Ajiteru, Olatunji, Sultan, Md Tipu, Lee, Ok Joo, Kim, Soon Hee, Park, Chan Hum
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013754/
https://www.ncbi.nlm.nih.gov/pubmed/36925698
http://dx.doi.org/10.1002/btm2.10423
_version_ 1784906842642579456
author Park, Hae Sang
Lee, Ji Seung
Kim, Chang‐Beom
Lee, Kwang‐Ho
Hong, In‐Sun
Jung, Harry
Lee, Hanna
Lee, Young Jin
Ajiteru, Olatunji
Sultan, Md Tipu
Lee, Ok Joo
Kim, Soon Hee
Park, Chan Hum
author_facet Park, Hae Sang
Lee, Ji Seung
Kim, Chang‐Beom
Lee, Kwang‐Ho
Hong, In‐Sun
Jung, Harry
Lee, Hanna
Lee, Young Jin
Ajiteru, Olatunji
Sultan, Md Tipu
Lee, Ok Joo
Kim, Soon Hee
Park, Chan Hum
author_sort Park, Hae Sang
collection PubMed
description Herein, we report the first study to create a three‐dimensional (3D) bioprinted artificial larynx for whole‐laryngeal replacement. Our 3D bio‐printed larynx was generated using extrusion‐based 3D bioprinter with rabbit's chondrocyte‐laden gelatin methacryloyl (GelMA)/glycidyl‐methacrylated hyaluronic acid (GMHA) hybrid bioink. We used a polycaprolactone (PCL) outer framework incorporated with pores to achieve the structural strength of printed constructs, as well as to provide a suitable microenvironment to support printed cells. Notably, we established a novel fluidics supply (FS) system that simultaneously supplies basal medium together with a 3D bioprinting process, thereby improving cell survival during the printing process. Our results showed that the FS system enhanced post‐printing cell viability, which enabled the generation of a large‐scale cell‐laden artificial laryngeal framework. Additionally, the incorporation of the PCL outer framework with pores and inner hydrogel provides structural stability and sufficient nutrient/oxygen transport. An animal study confirmed that the transplanted 3D bio‐larynx successfully maintained the airway. With further development, our new strategy holds great potential for fabricating human‐scale larynxes with in vivo‐like biological functions for laryngectomy patients.
format Online
Article
Text
id pubmed-10013754
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley & Sons, Inc.
record_format MEDLINE/PubMed
spelling pubmed-100137542023-03-15 Fluidic integrated 3D bioprinting system to sustain cell viability towards larynx fabrication Park, Hae Sang Lee, Ji Seung Kim, Chang‐Beom Lee, Kwang‐Ho Hong, In‐Sun Jung, Harry Lee, Hanna Lee, Young Jin Ajiteru, Olatunji Sultan, Md Tipu Lee, Ok Joo Kim, Soon Hee Park, Chan Hum Bioeng Transl Med Research Articles Herein, we report the first study to create a three‐dimensional (3D) bioprinted artificial larynx for whole‐laryngeal replacement. Our 3D bio‐printed larynx was generated using extrusion‐based 3D bioprinter with rabbit's chondrocyte‐laden gelatin methacryloyl (GelMA)/glycidyl‐methacrylated hyaluronic acid (GMHA) hybrid bioink. We used a polycaprolactone (PCL) outer framework incorporated with pores to achieve the structural strength of printed constructs, as well as to provide a suitable microenvironment to support printed cells. Notably, we established a novel fluidics supply (FS) system that simultaneously supplies basal medium together with a 3D bioprinting process, thereby improving cell survival during the printing process. Our results showed that the FS system enhanced post‐printing cell viability, which enabled the generation of a large‐scale cell‐laden artificial laryngeal framework. Additionally, the incorporation of the PCL outer framework with pores and inner hydrogel provides structural stability and sufficient nutrient/oxygen transport. An animal study confirmed that the transplanted 3D bio‐larynx successfully maintained the airway. With further development, our new strategy holds great potential for fabricating human‐scale larynxes with in vivo‐like biological functions for laryngectomy patients. John Wiley & Sons, Inc. 2022-10-20 /pmc/articles/PMC10013754/ /pubmed/36925698 http://dx.doi.org/10.1002/btm2.10423 Text en © 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Park, Hae Sang
Lee, Ji Seung
Kim, Chang‐Beom
Lee, Kwang‐Ho
Hong, In‐Sun
Jung, Harry
Lee, Hanna
Lee, Young Jin
Ajiteru, Olatunji
Sultan, Md Tipu
Lee, Ok Joo
Kim, Soon Hee
Park, Chan Hum
Fluidic integrated 3D bioprinting system to sustain cell viability towards larynx fabrication
title Fluidic integrated 3D bioprinting system to sustain cell viability towards larynx fabrication
title_full Fluidic integrated 3D bioprinting system to sustain cell viability towards larynx fabrication
title_fullStr Fluidic integrated 3D bioprinting system to sustain cell viability towards larynx fabrication
title_full_unstemmed Fluidic integrated 3D bioprinting system to sustain cell viability towards larynx fabrication
title_short Fluidic integrated 3D bioprinting system to sustain cell viability towards larynx fabrication
title_sort fluidic integrated 3d bioprinting system to sustain cell viability towards larynx fabrication
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013754/
https://www.ncbi.nlm.nih.gov/pubmed/36925698
http://dx.doi.org/10.1002/btm2.10423
work_keys_str_mv AT parkhaesang fluidicintegrated3dbioprintingsystemtosustaincellviabilitytowardslarynxfabrication
AT leejiseung fluidicintegrated3dbioprintingsystemtosustaincellviabilitytowardslarynxfabrication
AT kimchangbeom fluidicintegrated3dbioprintingsystemtosustaincellviabilitytowardslarynxfabrication
AT leekwangho fluidicintegrated3dbioprintingsystemtosustaincellviabilitytowardslarynxfabrication
AT honginsun fluidicintegrated3dbioprintingsystemtosustaincellviabilitytowardslarynxfabrication
AT jungharry fluidicintegrated3dbioprintingsystemtosustaincellviabilitytowardslarynxfabrication
AT leehanna fluidicintegrated3dbioprintingsystemtosustaincellviabilitytowardslarynxfabrication
AT leeyoungjin fluidicintegrated3dbioprintingsystemtosustaincellviabilitytowardslarynxfabrication
AT ajiteruolatunji fluidicintegrated3dbioprintingsystemtosustaincellviabilitytowardslarynxfabrication
AT sultanmdtipu fluidicintegrated3dbioprintingsystemtosustaincellviabilitytowardslarynxfabrication
AT leeokjoo fluidicintegrated3dbioprintingsystemtosustaincellviabilitytowardslarynxfabrication
AT kimsoonhee fluidicintegrated3dbioprintingsystemtosustaincellviabilitytowardslarynxfabrication
AT parkchanhum fluidicintegrated3dbioprintingsystemtosustaincellviabilitytowardslarynxfabrication