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Electrostatic potential difference between tumor and paratumor regulates cancer stem cell behavior and prognose tumor spread

Tumor spread is responsible for most deaths related to cancer. Increasing the accuracy of cancer prognosis is critical to reducing the high mortality rates in cancer patients. Here, we report that the electrostatic potential difference (EPD) between tumor and its paratumor tissue is a prognostic mar...

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Autores principales: Zhao, Haoran, Zhang, Weijie, Tang, Xiaowei, Galan, Edgar A., Zhu, Yinheng, Sang, Gan, Khutsishvili, Davit, Zheng, Honghui, Ma, Shaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013821/
https://www.ncbi.nlm.nih.gov/pubmed/36925705
http://dx.doi.org/10.1002/btm2.10399
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author Zhao, Haoran
Zhang, Weijie
Tang, Xiaowei
Galan, Edgar A.
Zhu, Yinheng
Sang, Gan
Khutsishvili, Davit
Zheng, Honghui
Ma, Shaohua
author_facet Zhao, Haoran
Zhang, Weijie
Tang, Xiaowei
Galan, Edgar A.
Zhu, Yinheng
Sang, Gan
Khutsishvili, Davit
Zheng, Honghui
Ma, Shaohua
author_sort Zhao, Haoran
collection PubMed
description Tumor spread is responsible for most deaths related to cancer. Increasing the accuracy of cancer prognosis is critical to reducing the high mortality rates in cancer patients. Here, we report that the electrostatic potential difference (EPD) between tumor and its paratumor tissue is a prognostic marker for tumor spread. This finding is concluded from the patient‐specific EPD values and clinical observation. The electrostatic potential values were measured on tissue cryosections from 51 patients using Kelvin probe force microscopy (KPFM). A total of ~44% (15/34) patients of V(tumor–paratumor) > 0 were featured with tumor spread, whereas only ~18% (2/11) patients of V(tumor–paratumor) < 0 had tumor spread. Next, we found the increased enrichment of cancer stem cells in paratumors with lower electrostatic potentials using immunofluorescence imaging, which suggested the attribution of tumor spread to the galvanotaxis of cancer stem cells (CSCs) toward lower potential. The findings were finally validated in breast and lung spheroid models composed of differentiated cancer cells and cancer stem cells at the ratio of 1:1 and embedded in Matrigel dopped with negative‐, neutral‐ and positive‐charged polymers and CSCs prefer to spread out of spheroids to lower electrostatic potential sites. This work may inspire the development of diagnostic and prognostic strategies targeting at tissue EPDs and CSCs for tumor therapy.
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spelling pubmed-100138212023-03-15 Electrostatic potential difference between tumor and paratumor regulates cancer stem cell behavior and prognose tumor spread Zhao, Haoran Zhang, Weijie Tang, Xiaowei Galan, Edgar A. Zhu, Yinheng Sang, Gan Khutsishvili, Davit Zheng, Honghui Ma, Shaohua Bioeng Transl Med Research Articles Tumor spread is responsible for most deaths related to cancer. Increasing the accuracy of cancer prognosis is critical to reducing the high mortality rates in cancer patients. Here, we report that the electrostatic potential difference (EPD) between tumor and its paratumor tissue is a prognostic marker for tumor spread. This finding is concluded from the patient‐specific EPD values and clinical observation. The electrostatic potential values were measured on tissue cryosections from 51 patients using Kelvin probe force microscopy (KPFM). A total of ~44% (15/34) patients of V(tumor–paratumor) > 0 were featured with tumor spread, whereas only ~18% (2/11) patients of V(tumor–paratumor) < 0 had tumor spread. Next, we found the increased enrichment of cancer stem cells in paratumors with lower electrostatic potentials using immunofluorescence imaging, which suggested the attribution of tumor spread to the galvanotaxis of cancer stem cells (CSCs) toward lower potential. The findings were finally validated in breast and lung spheroid models composed of differentiated cancer cells and cancer stem cells at the ratio of 1:1 and embedded in Matrigel dopped with negative‐, neutral‐ and positive‐charged polymers and CSCs prefer to spread out of spheroids to lower electrostatic potential sites. This work may inspire the development of diagnostic and prognostic strategies targeting at tissue EPDs and CSCs for tumor therapy. John Wiley & Sons, Inc. 2022-09-23 /pmc/articles/PMC10013821/ /pubmed/36925705 http://dx.doi.org/10.1002/btm2.10399 Text en © 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Zhao, Haoran
Zhang, Weijie
Tang, Xiaowei
Galan, Edgar A.
Zhu, Yinheng
Sang, Gan
Khutsishvili, Davit
Zheng, Honghui
Ma, Shaohua
Electrostatic potential difference between tumor and paratumor regulates cancer stem cell behavior and prognose tumor spread
title Electrostatic potential difference between tumor and paratumor regulates cancer stem cell behavior and prognose tumor spread
title_full Electrostatic potential difference between tumor and paratumor regulates cancer stem cell behavior and prognose tumor spread
title_fullStr Electrostatic potential difference between tumor and paratumor regulates cancer stem cell behavior and prognose tumor spread
title_full_unstemmed Electrostatic potential difference between tumor and paratumor regulates cancer stem cell behavior and prognose tumor spread
title_short Electrostatic potential difference between tumor and paratumor regulates cancer stem cell behavior and prognose tumor spread
title_sort electrostatic potential difference between tumor and paratumor regulates cancer stem cell behavior and prognose tumor spread
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013821/
https://www.ncbi.nlm.nih.gov/pubmed/36925705
http://dx.doi.org/10.1002/btm2.10399
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