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Natural Morin-Based Metal Organic Framework Nanoenzymes Modulate Articular Cavity Microenvironment to Alleviate Osteoarthritis
Osteoarthritis (OA) is always characterized as excessive reactive oxygen species (ROS) inside articular cavity. Mimicking natural metalloenzymes with metal ions as the active centers, stable metal organic framework (MOF) formed by natural polyphenols and metal ions shows great potential in alleviati...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AAAS
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013961/ https://www.ncbi.nlm.nih.gov/pubmed/36930778 http://dx.doi.org/10.34133/research.0068 |
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author | Cai, Jinhong Liu, Lian-feng Qin, Zainen Liu, Shuhan Wang, Yonglin Chen, Zhengrong Yao, Yi Zheng, Li Zhao, Jinmin Gao, Ming |
author_facet | Cai, Jinhong Liu, Lian-feng Qin, Zainen Liu, Shuhan Wang, Yonglin Chen, Zhengrong Yao, Yi Zheng, Li Zhao, Jinmin Gao, Ming |
author_sort | Cai, Jinhong |
collection | PubMed |
description | Osteoarthritis (OA) is always characterized as excessive reactive oxygen species (ROS) inside articular cavity. Mimicking natural metalloenzymes with metal ions as the active centers, stable metal organic framework (MOF) formed by natural polyphenols and metal ions shows great potential in alleviating inflammatory diseases. Herein, a series of novel copper-morin-based MOF (CuMHs) with different molar ratios of Cu(2+) and MH were employed to serve as ROS scavengers for OA therapy. As a result, CuMHs exhibited enhanced dispersion in aqueous solution, improved biocompatibility, and efficient ROS-scavenging ability compared to MH. On the basis of H(2)O(2)-stimulated chondrocytes, intracellular ROS levels were efficiently declined and cell death was prevented after treated by Cu(6)MH (Cu(2+) and MH molar ratio of 6:1). Meanwhile, Cu(6)MH also exhibited efficient antioxidant and anti-inflammation function by down-regulating the expression of IL6, MMP13, and MMP3, and up-regulating cartilage specific gene expression as well. Importantly, Cu(6)MH could repair mitochondrial function by increasing mitochondrial membrane potential, reducing the accumulation of calcium ions, as well as promoting ATP content production. In OA joint model, intra-articular (IA) injected Cu(6)MH suppressed the progression of OA. It endowed that Cu(6)MH might be promising nanoenzymes for the prevention and treatment of various inflammatory diseases. |
format | Online Article Text |
id | pubmed-10013961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | AAAS |
record_format | MEDLINE/PubMed |
spelling | pubmed-100139612023-03-15 Natural Morin-Based Metal Organic Framework Nanoenzymes Modulate Articular Cavity Microenvironment to Alleviate Osteoarthritis Cai, Jinhong Liu, Lian-feng Qin, Zainen Liu, Shuhan Wang, Yonglin Chen, Zhengrong Yao, Yi Zheng, Li Zhao, Jinmin Gao, Ming Research (Wash D C) Research Article Osteoarthritis (OA) is always characterized as excessive reactive oxygen species (ROS) inside articular cavity. Mimicking natural metalloenzymes with metal ions as the active centers, stable metal organic framework (MOF) formed by natural polyphenols and metal ions shows great potential in alleviating inflammatory diseases. Herein, a series of novel copper-morin-based MOF (CuMHs) with different molar ratios of Cu(2+) and MH were employed to serve as ROS scavengers for OA therapy. As a result, CuMHs exhibited enhanced dispersion in aqueous solution, improved biocompatibility, and efficient ROS-scavenging ability compared to MH. On the basis of H(2)O(2)-stimulated chondrocytes, intracellular ROS levels were efficiently declined and cell death was prevented after treated by Cu(6)MH (Cu(2+) and MH molar ratio of 6:1). Meanwhile, Cu(6)MH also exhibited efficient antioxidant and anti-inflammation function by down-regulating the expression of IL6, MMP13, and MMP3, and up-regulating cartilage specific gene expression as well. Importantly, Cu(6)MH could repair mitochondrial function by increasing mitochondrial membrane potential, reducing the accumulation of calcium ions, as well as promoting ATP content production. In OA joint model, intra-articular (IA) injected Cu(6)MH suppressed the progression of OA. It endowed that Cu(6)MH might be promising nanoenzymes for the prevention and treatment of various inflammatory diseases. AAAS 2023-03-09 /pmc/articles/PMC10013961/ /pubmed/36930778 http://dx.doi.org/10.34133/research.0068 Text en Copyright © 2023 Jinhong Cai et al. https://creativecommons.org/licenses/by/4.0/Exclusive licensee Science and Technology Review Publishing House. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Cai, Jinhong Liu, Lian-feng Qin, Zainen Liu, Shuhan Wang, Yonglin Chen, Zhengrong Yao, Yi Zheng, Li Zhao, Jinmin Gao, Ming Natural Morin-Based Metal Organic Framework Nanoenzymes Modulate Articular Cavity Microenvironment to Alleviate Osteoarthritis |
title | Natural Morin-Based Metal Organic Framework Nanoenzymes Modulate Articular Cavity Microenvironment to Alleviate Osteoarthritis |
title_full | Natural Morin-Based Metal Organic Framework Nanoenzymes Modulate Articular Cavity Microenvironment to Alleviate Osteoarthritis |
title_fullStr | Natural Morin-Based Metal Organic Framework Nanoenzymes Modulate Articular Cavity Microenvironment to Alleviate Osteoarthritis |
title_full_unstemmed | Natural Morin-Based Metal Organic Framework Nanoenzymes Modulate Articular Cavity Microenvironment to Alleviate Osteoarthritis |
title_short | Natural Morin-Based Metal Organic Framework Nanoenzymes Modulate Articular Cavity Microenvironment to Alleviate Osteoarthritis |
title_sort | natural morin-based metal organic framework nanoenzymes modulate articular cavity microenvironment to alleviate osteoarthritis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013961/ https://www.ncbi.nlm.nih.gov/pubmed/36930778 http://dx.doi.org/10.34133/research.0068 |
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