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Detection and genetic characterization of canine kobuvirus from stray dogs in Shanghai, China
In this study, rectal samples collected from 60 stray dogs in dog shelters were screened for canine kobuvirus and other enteroviruses by quantitative real-time reverse transcription polymerase chain reaction. Canine kobuvirus was detected in 25% (15/60) of the samples. In the 15 positive samples, th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013983/ https://www.ncbi.nlm.nih.gov/pubmed/36918497 http://dx.doi.org/10.1007/s00705-023-05710-z |
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author | Deng, Bo Song, Yuying Li, Liang Zhou, Yumen Zhu, Chunyan Zhang, Weiyi Feng, Dongsheng |
author_facet | Deng, Bo Song, Yuying Li, Liang Zhou, Yumen Zhu, Chunyan Zhang, Weiyi Feng, Dongsheng |
author_sort | Deng, Bo |
collection | PubMed |
description | In this study, rectal samples collected from 60 stray dogs in dog shelters were screened for canine kobuvirus and other enteroviruses by quantitative real-time reverse transcription polymerase chain reaction. Canine kobuvirus was detected in 25% (15/60) of the samples. In the 15 positive samples, the coinfection rates of canine distemper virus, canine coronavirus, canine astrovirus, canine norovirus, and canine rotavirus were 26.67%, 20.00%, 73.33%, 0%, and 20.00%, respectively. Phylogenetic analysis based on partial VP1 sequences identified a novel canine kobuvirus that was a recombinant of canine and feline kobuvirus. Bayesian evolutionary analysis revealed that the rate of evolution of the VP1 gene of canine kobuvirus was 1.36 × 10(−4) substitutions per site per year (95% highest posterior density interval, 6.28 × 10(−7) − 4.30 × 10(−4) substitutions per site per year). Finally, the divergence time of VP1 was around 19.44 years ago (95% highest posterior density interval, 12.96–27.57 years). SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-023-05710-z. |
format | Online Article Text |
id | pubmed-10013983 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-100139832023-03-15 Detection and genetic characterization of canine kobuvirus from stray dogs in Shanghai, China Deng, Bo Song, Yuying Li, Liang Zhou, Yumen Zhu, Chunyan Zhang, Weiyi Feng, Dongsheng Arch Virol Brief Report In this study, rectal samples collected from 60 stray dogs in dog shelters were screened for canine kobuvirus and other enteroviruses by quantitative real-time reverse transcription polymerase chain reaction. Canine kobuvirus was detected in 25% (15/60) of the samples. In the 15 positive samples, the coinfection rates of canine distemper virus, canine coronavirus, canine astrovirus, canine norovirus, and canine rotavirus were 26.67%, 20.00%, 73.33%, 0%, and 20.00%, respectively. Phylogenetic analysis based on partial VP1 sequences identified a novel canine kobuvirus that was a recombinant of canine and feline kobuvirus. Bayesian evolutionary analysis revealed that the rate of evolution of the VP1 gene of canine kobuvirus was 1.36 × 10(−4) substitutions per site per year (95% highest posterior density interval, 6.28 × 10(−7) − 4.30 × 10(−4) substitutions per site per year). Finally, the divergence time of VP1 was around 19.44 years ago (95% highest posterior density interval, 12.96–27.57 years). SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-023-05710-z. Springer Vienna 2023-03-14 2023 /pmc/articles/PMC10013983/ /pubmed/36918497 http://dx.doi.org/10.1007/s00705-023-05710-z Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2023 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Brief Report Deng, Bo Song, Yuying Li, Liang Zhou, Yumen Zhu, Chunyan Zhang, Weiyi Feng, Dongsheng Detection and genetic characterization of canine kobuvirus from stray dogs in Shanghai, China |
title | Detection and genetic characterization of canine kobuvirus from stray dogs in Shanghai, China |
title_full | Detection and genetic characterization of canine kobuvirus from stray dogs in Shanghai, China |
title_fullStr | Detection and genetic characterization of canine kobuvirus from stray dogs in Shanghai, China |
title_full_unstemmed | Detection and genetic characterization of canine kobuvirus from stray dogs in Shanghai, China |
title_short | Detection and genetic characterization of canine kobuvirus from stray dogs in Shanghai, China |
title_sort | detection and genetic characterization of canine kobuvirus from stray dogs in shanghai, china |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013983/ https://www.ncbi.nlm.nih.gov/pubmed/36918497 http://dx.doi.org/10.1007/s00705-023-05710-z |
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