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Host-Defense-Peptide-Mimicking β-Peptide Polymer Acting as a Dual-Modal Antibacterial Agent by Interfering Quorum Sensing and Killing Individual Bacteria Simultaneously

Host defense peptides (HDPs) are one of the potentially promising agents for infection diseases due to their broad spectrum and low resistance rate, but their clinical applications are limited by proteolytic instability, high-cost, and complicated synthesis process. Here, we report a host-defense-pe...

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Autores principales: Li, Wanlin, Xiao, Ximian, Qi, Yuchen, Lin, Xiuhui, Hu, Huiqun, Shi, Minqi, Zhou, Min, Jiang, Weinan, Liu, Longqiang, Chen, Kang, Wang, Kai, Liu, Runhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AAAS 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014070/
https://www.ncbi.nlm.nih.gov/pubmed/36930779
http://dx.doi.org/10.34133/research.0051
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author Li, Wanlin
Xiao, Ximian
Qi, Yuchen
Lin, Xiuhui
Hu, Huiqun
Shi, Minqi
Zhou, Min
Jiang, Weinan
Liu, Longqiang
Chen, Kang
Wang, Kai
Liu, Runhui
Zhou, Min
author_facet Li, Wanlin
Xiao, Ximian
Qi, Yuchen
Lin, Xiuhui
Hu, Huiqun
Shi, Minqi
Zhou, Min
Jiang, Weinan
Liu, Longqiang
Chen, Kang
Wang, Kai
Liu, Runhui
Zhou, Min
author_sort Li, Wanlin
collection PubMed
description Host defense peptides (HDPs) are one of the potentially promising agents for infection diseases due to their broad spectrum and low resistance rate, but their clinical applications are limited by proteolytic instability, high-cost, and complicated synthesis process. Here, we report a host-defense-peptide-mimicking β-peptide polymer that resists proteolysis to have enhanced the activity under physiological conditions, excellent antimicrobial efficiency even at high density of bacteria, and low cost for preparation. The β-peptide polymer demonstrated quorum sensing (QS) interference and bactericidal effect against both bacterial communities and individual bacterium to simultaneously block bacterial communication and disrupt bacterial membranes. The hierarchical QS network was suppressed, and main QS signaling systems showed considerably down-regulated gene expression, resulting in excellent biofilm eradication and virulence reduction effects. The dual-modal antibacterial ability possessed excellent therapeutic effects in Pseudomonas aeruginosa pneumonia, which could inhibit biofilm formation and exhibit better antibacterial and anti-inflammatory efficiency than clinically used antibiotics, levofloxacin. Furthermore, the β-peptide polymer also showed excellent therapeutic effect Escherichia coli pyogenic liver abscess. Together, we believed that the β-peptide polymer had a feasible clinical potential to treat bacterial infection diseases.
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spelling pubmed-100140702023-03-15 Host-Defense-Peptide-Mimicking β-Peptide Polymer Acting as a Dual-Modal Antibacterial Agent by Interfering Quorum Sensing and Killing Individual Bacteria Simultaneously Li, Wanlin Xiao, Ximian Qi, Yuchen Lin, Xiuhui Hu, Huiqun Shi, Minqi Zhou, Min Jiang, Weinan Liu, Longqiang Chen, Kang Wang, Kai Liu, Runhui Zhou, Min Research (Wash D C) Research Article Host defense peptides (HDPs) are one of the potentially promising agents for infection diseases due to their broad spectrum and low resistance rate, but their clinical applications are limited by proteolytic instability, high-cost, and complicated synthesis process. Here, we report a host-defense-peptide-mimicking β-peptide polymer that resists proteolysis to have enhanced the activity under physiological conditions, excellent antimicrobial efficiency even at high density of bacteria, and low cost for preparation. The β-peptide polymer demonstrated quorum sensing (QS) interference and bactericidal effect against both bacterial communities and individual bacterium to simultaneously block bacterial communication and disrupt bacterial membranes. The hierarchical QS network was suppressed, and main QS signaling systems showed considerably down-regulated gene expression, resulting in excellent biofilm eradication and virulence reduction effects. The dual-modal antibacterial ability possessed excellent therapeutic effects in Pseudomonas aeruginosa pneumonia, which could inhibit biofilm formation and exhibit better antibacterial and anti-inflammatory efficiency than clinically used antibiotics, levofloxacin. Furthermore, the β-peptide polymer also showed excellent therapeutic effect Escherichia coli pyogenic liver abscess. Together, we believed that the β-peptide polymer had a feasible clinical potential to treat bacterial infection diseases. AAAS 2023-03-14 2023 /pmc/articles/PMC10014070/ /pubmed/36930779 http://dx.doi.org/10.34133/research.0051 Text en https://creativecommons.org/licenses/by/4.0/Exclusive Licensee Science and Technology Review Publishing House. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Li, Wanlin
Xiao, Ximian
Qi, Yuchen
Lin, Xiuhui
Hu, Huiqun
Shi, Minqi
Zhou, Min
Jiang, Weinan
Liu, Longqiang
Chen, Kang
Wang, Kai
Liu, Runhui
Zhou, Min
Host-Defense-Peptide-Mimicking β-Peptide Polymer Acting as a Dual-Modal Antibacterial Agent by Interfering Quorum Sensing and Killing Individual Bacteria Simultaneously
title Host-Defense-Peptide-Mimicking β-Peptide Polymer Acting as a Dual-Modal Antibacterial Agent by Interfering Quorum Sensing and Killing Individual Bacteria Simultaneously
title_full Host-Defense-Peptide-Mimicking β-Peptide Polymer Acting as a Dual-Modal Antibacterial Agent by Interfering Quorum Sensing and Killing Individual Bacteria Simultaneously
title_fullStr Host-Defense-Peptide-Mimicking β-Peptide Polymer Acting as a Dual-Modal Antibacterial Agent by Interfering Quorum Sensing and Killing Individual Bacteria Simultaneously
title_full_unstemmed Host-Defense-Peptide-Mimicking β-Peptide Polymer Acting as a Dual-Modal Antibacterial Agent by Interfering Quorum Sensing and Killing Individual Bacteria Simultaneously
title_short Host-Defense-Peptide-Mimicking β-Peptide Polymer Acting as a Dual-Modal Antibacterial Agent by Interfering Quorum Sensing and Killing Individual Bacteria Simultaneously
title_sort host-defense-peptide-mimicking β-peptide polymer acting as a dual-modal antibacterial agent by interfering quorum sensing and killing individual bacteria simultaneously
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014070/
https://www.ncbi.nlm.nih.gov/pubmed/36930779
http://dx.doi.org/10.34133/research.0051
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