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Information transfer in mammalian glycan-based communication

Glycan-binding proteins, so-called lectins, are exposed on mammalian cell surfaces and decipher the information encoded within glycans translating it into biochemical signal transduction pathways in the cell. These glycan-lectin communication pathways are complex and difficult to analyze. However, q...

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Autores principales: Fuchsberger, Felix F, Kim, Dongyoon, Baranova, Natalia, Vrban, Hanka, Kagelmacher, Marten, Wawrzinek, Robert, Rademacher, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014076/
https://www.ncbi.nlm.nih.gov/pubmed/36803584
http://dx.doi.org/10.7554/eLife.69415
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author Fuchsberger, Felix F
Kim, Dongyoon
Baranova, Natalia
Vrban, Hanka
Kagelmacher, Marten
Wawrzinek, Robert
Rademacher, Christoph
author_facet Fuchsberger, Felix F
Kim, Dongyoon
Baranova, Natalia
Vrban, Hanka
Kagelmacher, Marten
Wawrzinek, Robert
Rademacher, Christoph
author_sort Fuchsberger, Felix F
collection PubMed
description Glycan-binding proteins, so-called lectins, are exposed on mammalian cell surfaces and decipher the information encoded within glycans translating it into biochemical signal transduction pathways in the cell. These glycan-lectin communication pathways are complex and difficult to analyze. However, quantitative data with single-cell resolution provide means to disentangle the associated signaling cascades. We chose C-type lectin receptors (CTLs) expressed on immune cells as a model system to study their capacity to transmit information encoded in glycans of incoming particles. In particular, we used nuclear factor kappa-B-reporter cell lines expressing DC-specific ICAM-3–grabbing nonintegrin (DC-SIGN), macrophage C-type lectin (MCL), dectin-1, dectin-2, and macrophage-inducible C-type lectin (MINCLE), as well as TNFαR and TLR-1&2 in monocytic cell lines and compared their transmission of glycan-encoded information. All receptors transmit information with similar signaling capacity, except dectin-2. This lectin was identified to be less efficient in information transmission compared to the other CTLs, and even when the sensitivity of the dectin-2 pathway was enhanced by overexpression of its co-receptor FcRγ, its transmitted information was not. Next, we expanded our investigation toward the integration of multiple signal transduction pathways including synergistic lectins, which is crucial during pathogen recognition. We show how the signaling capacity of lectin receptors using a similar signal transduction pathway (dectin-1 and dectin-2) is being integrated by compromising between the lectins. In contrast, co-expression of MCL synergistically enhanced the dectin-2 signaling capacity, particularly at low-glycan stimulant concentration. By using dectin-2 and other lectins as examples, we demonstrate how signaling capacity of dectin-2 is modulated in the presence of other lectins, and therefore, the findings provide insight into how immune cells translate glycan information using multivalent interactions.
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spelling pubmed-100140762023-03-15 Information transfer in mammalian glycan-based communication Fuchsberger, Felix F Kim, Dongyoon Baranova, Natalia Vrban, Hanka Kagelmacher, Marten Wawrzinek, Robert Rademacher, Christoph eLife Cell Biology Glycan-binding proteins, so-called lectins, are exposed on mammalian cell surfaces and decipher the information encoded within glycans translating it into biochemical signal transduction pathways in the cell. These glycan-lectin communication pathways are complex and difficult to analyze. However, quantitative data with single-cell resolution provide means to disentangle the associated signaling cascades. We chose C-type lectin receptors (CTLs) expressed on immune cells as a model system to study their capacity to transmit information encoded in glycans of incoming particles. In particular, we used nuclear factor kappa-B-reporter cell lines expressing DC-specific ICAM-3–grabbing nonintegrin (DC-SIGN), macrophage C-type lectin (MCL), dectin-1, dectin-2, and macrophage-inducible C-type lectin (MINCLE), as well as TNFαR and TLR-1&2 in monocytic cell lines and compared their transmission of glycan-encoded information. All receptors transmit information with similar signaling capacity, except dectin-2. This lectin was identified to be less efficient in information transmission compared to the other CTLs, and even when the sensitivity of the dectin-2 pathway was enhanced by overexpression of its co-receptor FcRγ, its transmitted information was not. Next, we expanded our investigation toward the integration of multiple signal transduction pathways including synergistic lectins, which is crucial during pathogen recognition. We show how the signaling capacity of lectin receptors using a similar signal transduction pathway (dectin-1 and dectin-2) is being integrated by compromising between the lectins. In contrast, co-expression of MCL synergistically enhanced the dectin-2 signaling capacity, particularly at low-glycan stimulant concentration. By using dectin-2 and other lectins as examples, we demonstrate how signaling capacity of dectin-2 is modulated in the presence of other lectins, and therefore, the findings provide insight into how immune cells translate glycan information using multivalent interactions. eLife Sciences Publications, Ltd 2023-02-20 /pmc/articles/PMC10014076/ /pubmed/36803584 http://dx.doi.org/10.7554/eLife.69415 Text en © 2023, Fuchsberger, Kim et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Fuchsberger, Felix F
Kim, Dongyoon
Baranova, Natalia
Vrban, Hanka
Kagelmacher, Marten
Wawrzinek, Robert
Rademacher, Christoph
Information transfer in mammalian glycan-based communication
title Information transfer in mammalian glycan-based communication
title_full Information transfer in mammalian glycan-based communication
title_fullStr Information transfer in mammalian glycan-based communication
title_full_unstemmed Information transfer in mammalian glycan-based communication
title_short Information transfer in mammalian glycan-based communication
title_sort information transfer in mammalian glycan-based communication
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014076/
https://www.ncbi.nlm.nih.gov/pubmed/36803584
http://dx.doi.org/10.7554/eLife.69415
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