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Molecular and spatial profiling of the paraventricular nucleus of the thalamus

The paraventricular nucleus of the thalamus (PVT) is known to regulate various cognitive and behavioral processes. However, while functional diversity among PVT circuits has often been linked to cellular differences, the molecular identity and spatial distribution of PVT cell types remain unclear. T...

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Autores principales: Gao, Claire, Gohel, Chiraag A, Leng, Yan, Ma, Jun, Goldman, David, Levine, Ariel J, Penzo, Mario A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014079/
https://www.ncbi.nlm.nih.gov/pubmed/36867023
http://dx.doi.org/10.7554/eLife.81818
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author Gao, Claire
Gohel, Chiraag A
Leng, Yan
Ma, Jun
Goldman, David
Levine, Ariel J
Penzo, Mario A
author_facet Gao, Claire
Gohel, Chiraag A
Leng, Yan
Ma, Jun
Goldman, David
Levine, Ariel J
Penzo, Mario A
author_sort Gao, Claire
collection PubMed
description The paraventricular nucleus of the thalamus (PVT) is known to regulate various cognitive and behavioral processes. However, while functional diversity among PVT circuits has often been linked to cellular differences, the molecular identity and spatial distribution of PVT cell types remain unclear. To address this gap, here we used single nucleus RNA sequencing (snRNA-seq) and identified five molecularly distinct PVT neuronal subtypes in the mouse brain. Additionally, multiplex fluorescent in situ hybridization of top marker genes revealed that PVT subtypes are organized by a combination of previously unidentified molecular gradients. Lastly, comparing our dataset with a recently published single-cell sequencing atlas of the thalamus yielded novel insight into the PVT’s connectivity with the cortex, including unexpected innervation of auditory and visual areas. This comparison also revealed that our data contains a largely non-overlapping transcriptomic map of multiple midline thalamic nuclei. Collectively, our findings uncover previously unknown features of the molecular diversity and anatomical organization of the PVT and provide a valuable resource for future investigations.
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spelling pubmed-100140792023-03-15 Molecular and spatial profiling of the paraventricular nucleus of the thalamus Gao, Claire Gohel, Chiraag A Leng, Yan Ma, Jun Goldman, David Levine, Ariel J Penzo, Mario A eLife Neuroscience The paraventricular nucleus of the thalamus (PVT) is known to regulate various cognitive and behavioral processes. However, while functional diversity among PVT circuits has often been linked to cellular differences, the molecular identity and spatial distribution of PVT cell types remain unclear. To address this gap, here we used single nucleus RNA sequencing (snRNA-seq) and identified five molecularly distinct PVT neuronal subtypes in the mouse brain. Additionally, multiplex fluorescent in situ hybridization of top marker genes revealed that PVT subtypes are organized by a combination of previously unidentified molecular gradients. Lastly, comparing our dataset with a recently published single-cell sequencing atlas of the thalamus yielded novel insight into the PVT’s connectivity with the cortex, including unexpected innervation of auditory and visual areas. This comparison also revealed that our data contains a largely non-overlapping transcriptomic map of multiple midline thalamic nuclei. Collectively, our findings uncover previously unknown features of the molecular diversity and anatomical organization of the PVT and provide a valuable resource for future investigations. eLife Sciences Publications, Ltd 2023-03-03 /pmc/articles/PMC10014079/ /pubmed/36867023 http://dx.doi.org/10.7554/eLife.81818 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication (https://creativecommons.org/publicdomain/zero/1.0/) .
spellingShingle Neuroscience
Gao, Claire
Gohel, Chiraag A
Leng, Yan
Ma, Jun
Goldman, David
Levine, Ariel J
Penzo, Mario A
Molecular and spatial profiling of the paraventricular nucleus of the thalamus
title Molecular and spatial profiling of the paraventricular nucleus of the thalamus
title_full Molecular and spatial profiling of the paraventricular nucleus of the thalamus
title_fullStr Molecular and spatial profiling of the paraventricular nucleus of the thalamus
title_full_unstemmed Molecular and spatial profiling of the paraventricular nucleus of the thalamus
title_short Molecular and spatial profiling of the paraventricular nucleus of the thalamus
title_sort molecular and spatial profiling of the paraventricular nucleus of the thalamus
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014079/
https://www.ncbi.nlm.nih.gov/pubmed/36867023
http://dx.doi.org/10.7554/eLife.81818
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