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Molecular and spatial profiling of the paraventricular nucleus of the thalamus
The paraventricular nucleus of the thalamus (PVT) is known to regulate various cognitive and behavioral processes. However, while functional diversity among PVT circuits has often been linked to cellular differences, the molecular identity and spatial distribution of PVT cell types remain unclear. T...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014079/ https://www.ncbi.nlm.nih.gov/pubmed/36867023 http://dx.doi.org/10.7554/eLife.81818 |
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author | Gao, Claire Gohel, Chiraag A Leng, Yan Ma, Jun Goldman, David Levine, Ariel J Penzo, Mario A |
author_facet | Gao, Claire Gohel, Chiraag A Leng, Yan Ma, Jun Goldman, David Levine, Ariel J Penzo, Mario A |
author_sort | Gao, Claire |
collection | PubMed |
description | The paraventricular nucleus of the thalamus (PVT) is known to regulate various cognitive and behavioral processes. However, while functional diversity among PVT circuits has often been linked to cellular differences, the molecular identity and spatial distribution of PVT cell types remain unclear. To address this gap, here we used single nucleus RNA sequencing (snRNA-seq) and identified five molecularly distinct PVT neuronal subtypes in the mouse brain. Additionally, multiplex fluorescent in situ hybridization of top marker genes revealed that PVT subtypes are organized by a combination of previously unidentified molecular gradients. Lastly, comparing our dataset with a recently published single-cell sequencing atlas of the thalamus yielded novel insight into the PVT’s connectivity with the cortex, including unexpected innervation of auditory and visual areas. This comparison also revealed that our data contains a largely non-overlapping transcriptomic map of multiple midline thalamic nuclei. Collectively, our findings uncover previously unknown features of the molecular diversity and anatomical organization of the PVT and provide a valuable resource for future investigations. |
format | Online Article Text |
id | pubmed-10014079 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-100140792023-03-15 Molecular and spatial profiling of the paraventricular nucleus of the thalamus Gao, Claire Gohel, Chiraag A Leng, Yan Ma, Jun Goldman, David Levine, Ariel J Penzo, Mario A eLife Neuroscience The paraventricular nucleus of the thalamus (PVT) is known to regulate various cognitive and behavioral processes. However, while functional diversity among PVT circuits has often been linked to cellular differences, the molecular identity and spatial distribution of PVT cell types remain unclear. To address this gap, here we used single nucleus RNA sequencing (snRNA-seq) and identified five molecularly distinct PVT neuronal subtypes in the mouse brain. Additionally, multiplex fluorescent in situ hybridization of top marker genes revealed that PVT subtypes are organized by a combination of previously unidentified molecular gradients. Lastly, comparing our dataset with a recently published single-cell sequencing atlas of the thalamus yielded novel insight into the PVT’s connectivity with the cortex, including unexpected innervation of auditory and visual areas. This comparison also revealed that our data contains a largely non-overlapping transcriptomic map of multiple midline thalamic nuclei. Collectively, our findings uncover previously unknown features of the molecular diversity and anatomical organization of the PVT and provide a valuable resource for future investigations. eLife Sciences Publications, Ltd 2023-03-03 /pmc/articles/PMC10014079/ /pubmed/36867023 http://dx.doi.org/10.7554/eLife.81818 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication (https://creativecommons.org/publicdomain/zero/1.0/) . |
spellingShingle | Neuroscience Gao, Claire Gohel, Chiraag A Leng, Yan Ma, Jun Goldman, David Levine, Ariel J Penzo, Mario A Molecular and spatial profiling of the paraventricular nucleus of the thalamus |
title | Molecular and spatial profiling of the paraventricular nucleus of the thalamus |
title_full | Molecular and spatial profiling of the paraventricular nucleus of the thalamus |
title_fullStr | Molecular and spatial profiling of the paraventricular nucleus of the thalamus |
title_full_unstemmed | Molecular and spatial profiling of the paraventricular nucleus of the thalamus |
title_short | Molecular and spatial profiling of the paraventricular nucleus of the thalamus |
title_sort | molecular and spatial profiling of the paraventricular nucleus of the thalamus |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014079/ https://www.ncbi.nlm.nih.gov/pubmed/36867023 http://dx.doi.org/10.7554/eLife.81818 |
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