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Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome
Tick bites have been shown to transmit a novel form of severe food allergy, the galactose-α-1,3-galactose (α-Gal) syndrome (AGS). Cellular responses to α-Gal in patients with AGS have, to date, not been thoroughly scrutinized. Therefore, we investigated T and B cell proliferation, activation, and cy...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Clinical Investigation
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014093/ https://www.ncbi.nlm.nih.gov/pubmed/36701195 http://dx.doi.org/10.1172/JCI158357 |
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author | Apostolovic, Danijela Grundström, Jeanette Kiewiet, Mensiena B. Gea Perusko, Marija Hamsten, Carl Starkhammar, Maria Paulie, Staffan van Hage, Marianne |
author_facet | Apostolovic, Danijela Grundström, Jeanette Kiewiet, Mensiena B. Gea Perusko, Marija Hamsten, Carl Starkhammar, Maria Paulie, Staffan van Hage, Marianne |
author_sort | Apostolovic, Danijela |
collection | PubMed |
description | Tick bites have been shown to transmit a novel form of severe food allergy, the galactose-α-1,3-galactose (α-Gal) syndrome (AGS). Cellular responses to α-Gal in patients with AGS have, to date, not been thoroughly scrutinized. Therefore, we investigated T and B cell proliferation, activation, and cytokine profiles in response to tick protein extract (TE) and α-Gal-free TE in patients with AGS and in healthy controls. T and B cells from both patients and controls proliferated in response to TE, but significantly more in patients with AGS. B cell proliferation, but not T cell proliferation, in patients with AGS was reduced by removing α-Gal from the TE. In addition, TE induced a clear Th2 cytokine profile in patients with AGS. Expression of CD23 by B cells correlated only to T cell proliferation. However, both B cell proliferation and CD23 expression were reduced when CD40L and IL-4 were blocked. A large portion of the IgG1 and IgE antibodies binding TE in patients with AGS were directed against the α-Gal epitope. We have, for what we believe to be the first time, investigated T and B cell responses to α-Gal carrying tick proteins in patients with AGS, which will be essential for the understanding of the immune response against an allergenic carbohydrate transmitted by ticks. |
format | Online Article Text |
id | pubmed-10014093 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Society for Clinical Investigation |
record_format | MEDLINE/PubMed |
spelling | pubmed-100140932023-03-15 Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome Apostolovic, Danijela Grundström, Jeanette Kiewiet, Mensiena B. Gea Perusko, Marija Hamsten, Carl Starkhammar, Maria Paulie, Staffan van Hage, Marianne J Clin Invest Research Article Tick bites have been shown to transmit a novel form of severe food allergy, the galactose-α-1,3-galactose (α-Gal) syndrome (AGS). Cellular responses to α-Gal in patients with AGS have, to date, not been thoroughly scrutinized. Therefore, we investigated T and B cell proliferation, activation, and cytokine profiles in response to tick protein extract (TE) and α-Gal-free TE in patients with AGS and in healthy controls. T and B cells from both patients and controls proliferated in response to TE, but significantly more in patients with AGS. B cell proliferation, but not T cell proliferation, in patients with AGS was reduced by removing α-Gal from the TE. In addition, TE induced a clear Th2 cytokine profile in patients with AGS. Expression of CD23 by B cells correlated only to T cell proliferation. However, both B cell proliferation and CD23 expression were reduced when CD40L and IL-4 were blocked. A large portion of the IgG1 and IgE antibodies binding TE in patients with AGS were directed against the α-Gal epitope. We have, for what we believe to be the first time, investigated T and B cell responses to α-Gal carrying tick proteins in patients with AGS, which will be essential for the understanding of the immune response against an allergenic carbohydrate transmitted by ticks. American Society for Clinical Investigation 2023-03-15 /pmc/articles/PMC10014093/ /pubmed/36701195 http://dx.doi.org/10.1172/JCI158357 Text en © 2023 Apostolovic et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Apostolovic, Danijela Grundström, Jeanette Kiewiet, Mensiena B. Gea Perusko, Marija Hamsten, Carl Starkhammar, Maria Paulie, Staffan van Hage, Marianne Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome |
title | Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome |
title_full | Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome |
title_fullStr | Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome |
title_full_unstemmed | Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome |
title_short | Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome |
title_sort | th2-skewed t cells correlate with b cell response to α-gal and tick antigens in α-gal syndrome |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014093/ https://www.ncbi.nlm.nih.gov/pubmed/36701195 http://dx.doi.org/10.1172/JCI158357 |
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