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In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions.
Stressosomes are signal-sensing and integration hubs identified in many bacteria. At present, the role of the stressosome has only been investigated in Gram-positive bacteria. This work represents the first in vivo characterisation of the stressosome in a Gram-negative bacterium, Vibrio vulnificus....
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014275/ https://www.ncbi.nlm.nih.gov/pubmed/36936406 http://dx.doi.org/10.1016/j.crmicr.2023.100186 |
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author | Cutugno, Laura Tamayo, Borja Khatabi Soliman Lens, Piet N.L. O'Byrne, Conor Pané-Farré, Jan Boyd, Aoife |
author_facet | Cutugno, Laura Tamayo, Borja Khatabi Soliman Lens, Piet N.L. O'Byrne, Conor Pané-Farré, Jan Boyd, Aoife |
author_sort | Cutugno, Laura |
collection | PubMed |
description | Stressosomes are signal-sensing and integration hubs identified in many bacteria. At present, the role of the stressosome has only been investigated in Gram-positive bacteria. This work represents the first in vivo characterisation of the stressosome in a Gram-negative bacterium, Vibrio vulnificus. Previous in vitro characterisation of the complex has led to the hypothesis of a complex involved in iron metabolism and control of c-di-GMP levels. We demonstrate that the stressosome is probably involved in reshaping the glucose metabolism in Fe- and nutrient-limited conditions and mutations of the locus affect the activation of the glyoxylate shunt. Moreover, we show that the stressosome is needed for the transcription of fleQ and to promote motility, consistent with the hypothesis that the stressosome is involved in regulating c-di-GMP. This report highlights the potential role of the stressosome in a Gram-negative bacterium, with implications for the metabolism and motility of this pathogen. |
format | Online Article Text |
id | pubmed-10014275 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-100142752023-03-16 In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions. Cutugno, Laura Tamayo, Borja Khatabi Soliman Lens, Piet N.L. O'Byrne, Conor Pané-Farré, Jan Boyd, Aoife Curr Res Microb Sci Research Paper Stressosomes are signal-sensing and integration hubs identified in many bacteria. At present, the role of the stressosome has only been investigated in Gram-positive bacteria. This work represents the first in vivo characterisation of the stressosome in a Gram-negative bacterium, Vibrio vulnificus. Previous in vitro characterisation of the complex has led to the hypothesis of a complex involved in iron metabolism and control of c-di-GMP levels. We demonstrate that the stressosome is probably involved in reshaping the glucose metabolism in Fe- and nutrient-limited conditions and mutations of the locus affect the activation of the glyoxylate shunt. Moreover, we show that the stressosome is needed for the transcription of fleQ and to promote motility, consistent with the hypothesis that the stressosome is involved in regulating c-di-GMP. This report highlights the potential role of the stressosome in a Gram-negative bacterium, with implications for the metabolism and motility of this pathogen. Elsevier 2023-03-02 /pmc/articles/PMC10014275/ /pubmed/36936406 http://dx.doi.org/10.1016/j.crmicr.2023.100186 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Paper Cutugno, Laura Tamayo, Borja Khatabi Soliman Lens, Piet N.L. O'Byrne, Conor Pané-Farré, Jan Boyd, Aoife In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions. |
title | In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions. |
title_full | In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions. |
title_fullStr | In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions. |
title_full_unstemmed | In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions. |
title_short | In vivo characterisation of the Vibrio vulnificus stressosome: A complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions. |
title_sort | in vivo characterisation of the vibrio vulnificus stressosome: a complex involved in reshaping glucose metabolism and motility regulation, in nutrient- and iron-limited growth conditions. |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014275/ https://www.ncbi.nlm.nih.gov/pubmed/36936406 http://dx.doi.org/10.1016/j.crmicr.2023.100186 |
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