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A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution
Efficient labeling of the vasculature is important for understanding the organization of vascular networks. Here, we propose VALID, a vessel-labeling method that enables visualization of vascular networks with tissue clearing and light-sheet microscopy. VALID transforms traditional lipophilic dye so...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014313/ https://www.ncbi.nlm.nih.gov/pubmed/36936073 http://dx.doi.org/10.1016/j.crmeth.2023.100407 |
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author | Zhu, Jingtan Liu, Xiaomei Xu, Jianyi Deng, Yating Wang, Pingfu Liu, Zhang Yang, Qihang Li, Dongyu Yu, Tingting Zhu, Dan |
author_facet | Zhu, Jingtan Liu, Xiaomei Xu, Jianyi Deng, Yating Wang, Pingfu Liu, Zhang Yang, Qihang Li, Dongyu Yu, Tingting Zhu, Dan |
author_sort | Zhu, Jingtan |
collection | PubMed |
description | Efficient labeling of the vasculature is important for understanding the organization of vascular networks. Here, we propose VALID, a vessel-labeling method that enables visualization of vascular networks with tissue clearing and light-sheet microscopy. VALID transforms traditional lipophilic dye solution into hydrogel by introducing gelatin and restrains the dye aggregation, resulting in complete and uniform vessel-labeling patterns with high signal-to-background ratios. VALID also enhances the compatibility of lipophilic dyes with solvent-based tissue-clearing protocols, which was hard to achieve previously. Using VALID, we combined lipophilic dyes with solvent-based tissue-clearing techniques to perform 3D reconstructions of vasculature within mouse brain and spinal cord. We also employed VALID for 3D visualization and quantification of microvascular damage in a middle cerebral artery occlusion mouse model. VALID should provide a simple, cost-effective vessel-labeling protocol that would significantly widen the applications of lipophilic dyes in research on cerebrovascular complications. |
format | Online Article Text |
id | pubmed-10014313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-100143132023-03-16 A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution Zhu, Jingtan Liu, Xiaomei Xu, Jianyi Deng, Yating Wang, Pingfu Liu, Zhang Yang, Qihang Li, Dongyu Yu, Tingting Zhu, Dan Cell Rep Methods Article Efficient labeling of the vasculature is important for understanding the organization of vascular networks. Here, we propose VALID, a vessel-labeling method that enables visualization of vascular networks with tissue clearing and light-sheet microscopy. VALID transforms traditional lipophilic dye solution into hydrogel by introducing gelatin and restrains the dye aggregation, resulting in complete and uniform vessel-labeling patterns with high signal-to-background ratios. VALID also enhances the compatibility of lipophilic dyes with solvent-based tissue-clearing protocols, which was hard to achieve previously. Using VALID, we combined lipophilic dyes with solvent-based tissue-clearing techniques to perform 3D reconstructions of vasculature within mouse brain and spinal cord. We also employed VALID for 3D visualization and quantification of microvascular damage in a middle cerebral artery occlusion mouse model. VALID should provide a simple, cost-effective vessel-labeling protocol that would significantly widen the applications of lipophilic dyes in research on cerebrovascular complications. Elsevier 2023-02-08 /pmc/articles/PMC10014313/ /pubmed/36936073 http://dx.doi.org/10.1016/j.crmeth.2023.100407 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Zhu, Jingtan Liu, Xiaomei Xu, Jianyi Deng, Yating Wang, Pingfu Liu, Zhang Yang, Qihang Li, Dongyu Yu, Tingting Zhu, Dan A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution |
title | A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution |
title_full | A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution |
title_fullStr | A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution |
title_full_unstemmed | A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution |
title_short | A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution |
title_sort | versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014313/ https://www.ncbi.nlm.nih.gov/pubmed/36936073 http://dx.doi.org/10.1016/j.crmeth.2023.100407 |
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