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The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers
Our previous study demonstrated that the zinc (Zn) proteinate with moderate chelation strength (Zn-Prot M) enhanced the Zn absorption in the small intestine partially via increasing the expression of some Zn and amino acid transporters in the duodenum of broilers. However, it remains unknown whether...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014343/ https://www.ncbi.nlm.nih.gov/pubmed/36680861 http://dx.doi.org/10.1016/j.psj.2023.102477 |
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author | Hu, Yun Huang, Yanhui Wang, Chuanlong Zhang, Weiyun Qu, Yicheng Li, Ding Wu, Wei Gao, Feiyu Zhu, Ling Wu, Bingxin Zhang, Liyang Cui, Xiaoyan Li, Tingting Geng, Yanqiang Liao, Xiudong Luo, Xugang |
author_facet | Hu, Yun Huang, Yanhui Wang, Chuanlong Zhang, Weiyun Qu, Yicheng Li, Ding Wu, Wei Gao, Feiyu Zhu, Ling Wu, Bingxin Zhang, Liyang Cui, Xiaoyan Li, Tingting Geng, Yanqiang Liao, Xiudong Luo, Xugang |
author_sort | Hu, Yun |
collection | PubMed |
description | Our previous study demonstrated that the zinc (Zn) proteinate with moderate chelation strength (Zn-Prot M) enhanced the Zn absorption in the small intestine partially via increasing the expression of some Zn and amino acid transporters in the duodenum of broilers. However, it remains unknown whether the Zn-Prot M could also regulate the expression of related transporters in the jejunum and ileum of broilers in the above enhancement of Zn absorption. The present study was conducted to investigate the effect of the Zn-Prot M on the expression of related transporters in the jejunum and ileum of broilers compared to the Zn sulfate (ZnS). Zinc-deficient broilers (13-d-old) were fed with the Zn-unsupplemented basal diets (control) or the basal diets supplemented with 60 mg Zn/kg as ZnS or Zn-Prot M for 26 d. The results showed that in the jejunum, compared to the control, supplementation of the organic or inorganic Zn increased (P < 0.05) mRNA and protein expression of b(0,+)-type amino acid transporter (rBAT), Zn transporter 10 (ZnT10), and peptide-transporter 1 (PepT1) mRNA expression and Zn transporter 7 (ZnT7) protein expression on d 28, while y+L-type amino transporter 2 (y+LAT2) mRNA and protein expression, and protein expression of ZnT7 and ZnT10 on 28 d and zrt-irt-like protein 3 (ZIP3) and zrt-irt-like protein 5 (ZIP5) on d 39 were higher (P < 0.05) for Zn-Prot M than for ZnS. In the ileum, Zn addition regardless of Zn source up-regulated (P < 0.05) mRNA expression of Zn transporter 9 (ZnT9) and ZIP3, ZIP5, and y+LAT2 protein expression on d 28, and PepT1 mRNA and protein expression, ZIP3 and y+LAT2 mRNA expression and ZnT10 protein expression on d 39. Furthermore, Zn transporter 4 (ZnT4) and ZnT9 mRNA expression and Zn transporter 1 (ZnT1) protein expression on d 28, and y+LAT2 mRNA expression and ZnT10 and PepT1 protein expression on d 39 were higher (P < 0.05) for Zn-Prot M than for ZnS. It was concluded that the Zn-Prot M enhanced the expression of the ZnT1, ZnT4, ZnT9, ZnT10, ZIP3, ZIP5, y+LAT2, and PepT1 in the jejunum or ileum of broilers compared to the ZnS. |
format | Online Article Text |
id | pubmed-10014343 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-100143432023-03-16 The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers Hu, Yun Huang, Yanhui Wang, Chuanlong Zhang, Weiyun Qu, Yicheng Li, Ding Wu, Wei Gao, Feiyu Zhu, Ling Wu, Bingxin Zhang, Liyang Cui, Xiaoyan Li, Tingting Geng, Yanqiang Liao, Xiudong Luo, Xugang Poult Sci METABOLISM AND NUTRITION Our previous study demonstrated that the zinc (Zn) proteinate with moderate chelation strength (Zn-Prot M) enhanced the Zn absorption in the small intestine partially via increasing the expression of some Zn and amino acid transporters in the duodenum of broilers. However, it remains unknown whether the Zn-Prot M could also regulate the expression of related transporters in the jejunum and ileum of broilers in the above enhancement of Zn absorption. The present study was conducted to investigate the effect of the Zn-Prot M on the expression of related transporters in the jejunum and ileum of broilers compared to the Zn sulfate (ZnS). Zinc-deficient broilers (13-d-old) were fed with the Zn-unsupplemented basal diets (control) or the basal diets supplemented with 60 mg Zn/kg as ZnS or Zn-Prot M for 26 d. The results showed that in the jejunum, compared to the control, supplementation of the organic or inorganic Zn increased (P < 0.05) mRNA and protein expression of b(0,+)-type amino acid transporter (rBAT), Zn transporter 10 (ZnT10), and peptide-transporter 1 (PepT1) mRNA expression and Zn transporter 7 (ZnT7) protein expression on d 28, while y+L-type amino transporter 2 (y+LAT2) mRNA and protein expression, and protein expression of ZnT7 and ZnT10 on 28 d and zrt-irt-like protein 3 (ZIP3) and zrt-irt-like protein 5 (ZIP5) on d 39 were higher (P < 0.05) for Zn-Prot M than for ZnS. In the ileum, Zn addition regardless of Zn source up-regulated (P < 0.05) mRNA expression of Zn transporter 9 (ZnT9) and ZIP3, ZIP5, and y+LAT2 protein expression on d 28, and PepT1 mRNA and protein expression, ZIP3 and y+LAT2 mRNA expression and ZnT10 protein expression on d 39. Furthermore, Zn transporter 4 (ZnT4) and ZnT9 mRNA expression and Zn transporter 1 (ZnT1) protein expression on d 28, and y+LAT2 mRNA expression and ZnT10 and PepT1 protein expression on d 39 were higher (P < 0.05) for Zn-Prot M than for ZnS. It was concluded that the Zn-Prot M enhanced the expression of the ZnT1, ZnT4, ZnT9, ZnT10, ZIP3, ZIP5, y+LAT2, and PepT1 in the jejunum or ileum of broilers compared to the ZnS. Elsevier 2023-01-06 /pmc/articles/PMC10014343/ /pubmed/36680861 http://dx.doi.org/10.1016/j.psj.2023.102477 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | METABOLISM AND NUTRITION Hu, Yun Huang, Yanhui Wang, Chuanlong Zhang, Weiyun Qu, Yicheng Li, Ding Wu, Wei Gao, Feiyu Zhu, Ling Wu, Bingxin Zhang, Liyang Cui, Xiaoyan Li, Tingting Geng, Yanqiang Liao, Xiudong Luo, Xugang The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers |
title | The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers |
title_full | The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers |
title_fullStr | The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers |
title_full_unstemmed | The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers |
title_short | The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers |
title_sort | organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers |
topic | METABOLISM AND NUTRITION |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014343/ https://www.ncbi.nlm.nih.gov/pubmed/36680861 http://dx.doi.org/10.1016/j.psj.2023.102477 |
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