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Transcriptome analysis provides insights into the response of Lotus corniculatus roots to low-phosphorus stress

INTRODUCTION: A lack of soil phosphorus (P) is a principal factor restricting the normal growth of Lotus corniculatus in the karst area of Guizhou Province, China, but the response mechanism of L. corniculatus under low-phosphorus stress remains unclear. METHODS: Therefore, we treated two selected L...

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Autores principales: Zhao, Xin, Chen, Ke-ke, Wang, Lei-ting, Zhao, Li-Li, Wang, Pu-Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014540/
https://www.ncbi.nlm.nih.gov/pubmed/36938008
http://dx.doi.org/10.3389/fpls.2023.1089380
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author Zhao, Xin
Chen, Ke-ke
Wang, Lei-ting
Zhao, Li-Li
Wang, Pu-Chang
author_facet Zhao, Xin
Chen, Ke-ke
Wang, Lei-ting
Zhao, Li-Li
Wang, Pu-Chang
author_sort Zhao, Xin
collection PubMed
description INTRODUCTION: A lack of soil phosphorus (P) is a principal factor restricting the normal growth of Lotus corniculatus in the karst area of Guizhou Province, China, but the response mechanism of L. corniculatus under low-phosphorus stress remains unclear. METHODS: Therefore, we treated two selected L. corniculatus lines (low-P-intolerant line 08518 and low-P-tolerant line 01549) from 13 L. corniculatus lines with normal phosphorus (0.5 mmol/L KH(2)PO(4), NP) and low phosphorus (0.005 mmol/L KH(2)PO(4), LP) concentrations to study changes in morphological, physiological and transcriptome data under low-phosphorus stress. RESULTS: The low-P-tolerant line 01549 exhibited better performance under low-phosphorus stress. Compared with the NP treatment, all root morphological indicators of the low-P-tolerant line 01549 increased, and those of the low-P-intolerant line 08518 decreased under low-P stress. Compared with the NP treatment, acid phosphatase (ACP), catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) activities, and the malondialdehyde (MDA), soluble sugar (SS), soluble protein (SP) and proline (Pro) contents of the two L. corniculatus lines increased under low-P stress. A transcriptome analysis of L. corniculatus showed that a total of 656 and 2243 differentially expressed genes (DEGs) were identified in line 01549 and line 08518, respectively. Meanwhile, the main pathways, such as carbohydrate metabolism, acid phosphatases, phosphate transporters and biosynthesis of secondary metabolites, as well as related genes were also screened by performing a KEGG enrichment analysis. DISCUSSION: The findings provide an essential point of reference for studying the physiological and molecular mechanism of the response to low-P stress in L. corniculatus.
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spelling pubmed-100145402023-03-16 Transcriptome analysis provides insights into the response of Lotus corniculatus roots to low-phosphorus stress Zhao, Xin Chen, Ke-ke Wang, Lei-ting Zhao, Li-Li Wang, Pu-Chang Front Plant Sci Plant Science INTRODUCTION: A lack of soil phosphorus (P) is a principal factor restricting the normal growth of Lotus corniculatus in the karst area of Guizhou Province, China, but the response mechanism of L. corniculatus under low-phosphorus stress remains unclear. METHODS: Therefore, we treated two selected L. corniculatus lines (low-P-intolerant line 08518 and low-P-tolerant line 01549) from 13 L. corniculatus lines with normal phosphorus (0.5 mmol/L KH(2)PO(4), NP) and low phosphorus (0.005 mmol/L KH(2)PO(4), LP) concentrations to study changes in morphological, physiological and transcriptome data under low-phosphorus stress. RESULTS: The low-P-tolerant line 01549 exhibited better performance under low-phosphorus stress. Compared with the NP treatment, all root morphological indicators of the low-P-tolerant line 01549 increased, and those of the low-P-intolerant line 08518 decreased under low-P stress. Compared with the NP treatment, acid phosphatase (ACP), catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) activities, and the malondialdehyde (MDA), soluble sugar (SS), soluble protein (SP) and proline (Pro) contents of the two L. corniculatus lines increased under low-P stress. A transcriptome analysis of L. corniculatus showed that a total of 656 and 2243 differentially expressed genes (DEGs) were identified in line 01549 and line 08518, respectively. Meanwhile, the main pathways, such as carbohydrate metabolism, acid phosphatases, phosphate transporters and biosynthesis of secondary metabolites, as well as related genes were also screened by performing a KEGG enrichment analysis. DISCUSSION: The findings provide an essential point of reference for studying the physiological and molecular mechanism of the response to low-P stress in L. corniculatus. Frontiers Media S.A. 2023-03-01 /pmc/articles/PMC10014540/ /pubmed/36938008 http://dx.doi.org/10.3389/fpls.2023.1089380 Text en Copyright © 2023 Zhao, Chen, Wang, Zhao and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhao, Xin
Chen, Ke-ke
Wang, Lei-ting
Zhao, Li-Li
Wang, Pu-Chang
Transcriptome analysis provides insights into the response of Lotus corniculatus roots to low-phosphorus stress
title Transcriptome analysis provides insights into the response of Lotus corniculatus roots to low-phosphorus stress
title_full Transcriptome analysis provides insights into the response of Lotus corniculatus roots to low-phosphorus stress
title_fullStr Transcriptome analysis provides insights into the response of Lotus corniculatus roots to low-phosphorus stress
title_full_unstemmed Transcriptome analysis provides insights into the response of Lotus corniculatus roots to low-phosphorus stress
title_short Transcriptome analysis provides insights into the response of Lotus corniculatus roots to low-phosphorus stress
title_sort transcriptome analysis provides insights into the response of lotus corniculatus roots to low-phosphorus stress
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014540/
https://www.ncbi.nlm.nih.gov/pubmed/36938008
http://dx.doi.org/10.3389/fpls.2023.1089380
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