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Optimization and standardization of mNGS-based procedures for the diagnosis of Mycoplasma periprosthetic joint infection: A novel diagnostic strategy for rare bacterial periprosthetic joint infection
INTRODUCTION: The diagnosis of Mycoplasma periprosthetic joint infection (PJI) is rather difficult due to its rarity and difficult in isolation, there are not standardized diagnostic procedure for Mycoplasma PJI presently. This study aimed to reported a metagenomic next-generation sequencing (mNGS)-...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014592/ https://www.ncbi.nlm.nih.gov/pubmed/36936762 http://dx.doi.org/10.3389/fcimb.2023.1089919 |
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author | Cai, Yuanqing Ding, Haiqi Chen, Xiaoqing Chen, Yang Huang, Changyu Zhang, Chaofan Huang, Zida Huang, Ying Li, Wenbo Zhang, Wenming Fang, Xinyu |
author_facet | Cai, Yuanqing Ding, Haiqi Chen, Xiaoqing Chen, Yang Huang, Changyu Zhang, Chaofan Huang, Zida Huang, Ying Li, Wenbo Zhang, Wenming Fang, Xinyu |
author_sort | Cai, Yuanqing |
collection | PubMed |
description | INTRODUCTION: The diagnosis of Mycoplasma periprosthetic joint infection (PJI) is rather difficult due to its rarity and difficult in isolation, there are not standardized diagnostic procedure for Mycoplasma PJI presently. This study aimed to reported a metagenomic next-generation sequencing (mNGS)-based diagnostic strategy for Mycoplasma PJI. METHODS: In the present study, we have reported the largest number of Mycoplasma PJI that were precisely diagnosed by mNGS and verified by optimized microbial culture methods and (or) 16S PCR polymerase chain reaction (PCR). RESULTS: The positive rate of optimized microbial culture methods and 16S PCR in the detection of Mycoplasma PJI was 57.14% and 71.43%, respectively. The infections were well controlled by targeted treatment in all cases. CONCLUSION: The standardized and optimized procedure based on mNGS presented in this study is useful for the diagnosis of Mycoplasma PJI, which might also be provided as a novel diagnostic strategy for rare bacterial PJI. |
format | Online Article Text |
id | pubmed-10014592 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100145922023-03-16 Optimization and standardization of mNGS-based procedures for the diagnosis of Mycoplasma periprosthetic joint infection: A novel diagnostic strategy for rare bacterial periprosthetic joint infection Cai, Yuanqing Ding, Haiqi Chen, Xiaoqing Chen, Yang Huang, Changyu Zhang, Chaofan Huang, Zida Huang, Ying Li, Wenbo Zhang, Wenming Fang, Xinyu Front Cell Infect Microbiol Cellular and Infection Microbiology INTRODUCTION: The diagnosis of Mycoplasma periprosthetic joint infection (PJI) is rather difficult due to its rarity and difficult in isolation, there are not standardized diagnostic procedure for Mycoplasma PJI presently. This study aimed to reported a metagenomic next-generation sequencing (mNGS)-based diagnostic strategy for Mycoplasma PJI. METHODS: In the present study, we have reported the largest number of Mycoplasma PJI that were precisely diagnosed by mNGS and verified by optimized microbial culture methods and (or) 16S PCR polymerase chain reaction (PCR). RESULTS: The positive rate of optimized microbial culture methods and 16S PCR in the detection of Mycoplasma PJI was 57.14% and 71.43%, respectively. The infections were well controlled by targeted treatment in all cases. CONCLUSION: The standardized and optimized procedure based on mNGS presented in this study is useful for the diagnosis of Mycoplasma PJI, which might also be provided as a novel diagnostic strategy for rare bacterial PJI. Frontiers Media S.A. 2023-03-01 /pmc/articles/PMC10014592/ /pubmed/36936762 http://dx.doi.org/10.3389/fcimb.2023.1089919 Text en Copyright © 2023 Cai, Ding, Chen, Chen, Huang, Zhang, Huang, Huang, Li, Zhang and Fang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Cai, Yuanqing Ding, Haiqi Chen, Xiaoqing Chen, Yang Huang, Changyu Zhang, Chaofan Huang, Zida Huang, Ying Li, Wenbo Zhang, Wenming Fang, Xinyu Optimization and standardization of mNGS-based procedures for the diagnosis of Mycoplasma periprosthetic joint infection: A novel diagnostic strategy for rare bacterial periprosthetic joint infection |
title | Optimization and standardization of mNGS-based procedures for the diagnosis of Mycoplasma periprosthetic joint infection: A novel diagnostic strategy for rare bacterial periprosthetic joint infection |
title_full | Optimization and standardization of mNGS-based procedures for the diagnosis of Mycoplasma periprosthetic joint infection: A novel diagnostic strategy for rare bacterial periprosthetic joint infection |
title_fullStr | Optimization and standardization of mNGS-based procedures for the diagnosis of Mycoplasma periprosthetic joint infection: A novel diagnostic strategy for rare bacterial periprosthetic joint infection |
title_full_unstemmed | Optimization and standardization of mNGS-based procedures for the diagnosis of Mycoplasma periprosthetic joint infection: A novel diagnostic strategy for rare bacterial periprosthetic joint infection |
title_short | Optimization and standardization of mNGS-based procedures for the diagnosis of Mycoplasma periprosthetic joint infection: A novel diagnostic strategy for rare bacterial periprosthetic joint infection |
title_sort | optimization and standardization of mngs-based procedures for the diagnosis of mycoplasma periprosthetic joint infection: a novel diagnostic strategy for rare bacterial periprosthetic joint infection |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014592/ https://www.ncbi.nlm.nih.gov/pubmed/36936762 http://dx.doi.org/10.3389/fcimb.2023.1089919 |
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