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Molecular characteristics and antibiotic resistance mechanisms of clindamycin-resistant Streptococcus agalactiae isolates in China
Streptococcus agalactiae (Group B Streptococcus, GBS) is a major cause of neonatal infections with high morbidity and mortality, and clindamycin is the main antibiotic used to treat GBS infections in patients allergic to penicillin. We aimed to analyse the antibiotic sensitivity, sequence types, ser...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014864/ https://www.ncbi.nlm.nih.gov/pubmed/36937303 http://dx.doi.org/10.3389/fmicb.2023.1138039 |
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author | Liu, Zeliang Jiang, Xueqi Li, Jie Ji, Wenjing Zhou, Haijian Gong, Xinyi Miao, Beibei Meng, Shuang Duan, Like Shi, Qiyuan Han, Xiao Gao, Pengfang Chang, Chienyi Dong, Aiying Li, Juan |
author_facet | Liu, Zeliang Jiang, Xueqi Li, Jie Ji, Wenjing Zhou, Haijian Gong, Xinyi Miao, Beibei Meng, Shuang Duan, Like Shi, Qiyuan Han, Xiao Gao, Pengfang Chang, Chienyi Dong, Aiying Li, Juan |
author_sort | Liu, Zeliang |
collection | PubMed |
description | Streptococcus agalactiae (Group B Streptococcus, GBS) is a major cause of neonatal infections with high morbidity and mortality, and clindamycin is the main antibiotic used to treat GBS infections in patients allergic to penicillin. We aimed to analyse the antibiotic sensitivity, sequence types, serotypes, virulence factors, and antibiotic resistance mechanisms of clinically isolated clindamycin-resistant S. agalactiae and provide basic data for the treatment, prevention, and control of clinical infection of S. agalactiae. A total of 110 strains of clindamycin-resistant S. agalactiae were collected from two tertiary hospitals in Hebei, China. We performed antibiotic sensitivity tests for 11 antibiotics on these strains and whole-genome sequencing analysis. All the strains were susceptible to penicillin, ampicillin, linezolid, vancomycin, tigecycline, and quinupristin–dalfopristin. Resistance to erythromycin, levofloxacin, tetracycline, and chloramphenicol were also observed. Genome sequence analysis revealed that all strains belonged to 12 sequence types (STs) related to six cloning complexes (CCs), namely CC10, CC19, CC23, CC651, CC1, and CC17. Five serotypes were identified, including IA, IB, II, III, and V. The most prominent resistance genes were mreA (100%) and ermB (81.8%). Furthermore, cfb, cylE, pavA and the gene cluster related to the pili were 100% present in all strains, followed by lmb (95.5%) and srr1 (67.2%). This study found that clindamycin-resistant S. agalactiae showed polymorphisms in molecular types and serotypes. Furthermore, multiple virulence factor genes have been identified in their genomes. |
format | Online Article Text |
id | pubmed-10014864 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100148642023-03-16 Molecular characteristics and antibiotic resistance mechanisms of clindamycin-resistant Streptococcus agalactiae isolates in China Liu, Zeliang Jiang, Xueqi Li, Jie Ji, Wenjing Zhou, Haijian Gong, Xinyi Miao, Beibei Meng, Shuang Duan, Like Shi, Qiyuan Han, Xiao Gao, Pengfang Chang, Chienyi Dong, Aiying Li, Juan Front Microbiol Microbiology Streptococcus agalactiae (Group B Streptococcus, GBS) is a major cause of neonatal infections with high morbidity and mortality, and clindamycin is the main antibiotic used to treat GBS infections in patients allergic to penicillin. We aimed to analyse the antibiotic sensitivity, sequence types, serotypes, virulence factors, and antibiotic resistance mechanisms of clinically isolated clindamycin-resistant S. agalactiae and provide basic data for the treatment, prevention, and control of clinical infection of S. agalactiae. A total of 110 strains of clindamycin-resistant S. agalactiae were collected from two tertiary hospitals in Hebei, China. We performed antibiotic sensitivity tests for 11 antibiotics on these strains and whole-genome sequencing analysis. All the strains were susceptible to penicillin, ampicillin, linezolid, vancomycin, tigecycline, and quinupristin–dalfopristin. Resistance to erythromycin, levofloxacin, tetracycline, and chloramphenicol were also observed. Genome sequence analysis revealed that all strains belonged to 12 sequence types (STs) related to six cloning complexes (CCs), namely CC10, CC19, CC23, CC651, CC1, and CC17. Five serotypes were identified, including IA, IB, II, III, and V. The most prominent resistance genes were mreA (100%) and ermB (81.8%). Furthermore, cfb, cylE, pavA and the gene cluster related to the pili were 100% present in all strains, followed by lmb (95.5%) and srr1 (67.2%). This study found that clindamycin-resistant S. agalactiae showed polymorphisms in molecular types and serotypes. Furthermore, multiple virulence factor genes have been identified in their genomes. Frontiers Media S.A. 2023-03-01 /pmc/articles/PMC10014864/ /pubmed/36937303 http://dx.doi.org/10.3389/fmicb.2023.1138039 Text en Copyright © 2023 Liu, Jiang, Li, Ji, Zhou, Gong, Miao, Meng, Duan, Shi, Han, Gao, Chang, Dong and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Liu, Zeliang Jiang, Xueqi Li, Jie Ji, Wenjing Zhou, Haijian Gong, Xinyi Miao, Beibei Meng, Shuang Duan, Like Shi, Qiyuan Han, Xiao Gao, Pengfang Chang, Chienyi Dong, Aiying Li, Juan Molecular characteristics and antibiotic resistance mechanisms of clindamycin-resistant Streptococcus agalactiae isolates in China |
title | Molecular characteristics and antibiotic resistance mechanisms of clindamycin-resistant Streptococcus agalactiae isolates in China |
title_full | Molecular characteristics and antibiotic resistance mechanisms of clindamycin-resistant Streptococcus agalactiae isolates in China |
title_fullStr | Molecular characteristics and antibiotic resistance mechanisms of clindamycin-resistant Streptococcus agalactiae isolates in China |
title_full_unstemmed | Molecular characteristics and antibiotic resistance mechanisms of clindamycin-resistant Streptococcus agalactiae isolates in China |
title_short | Molecular characteristics and antibiotic resistance mechanisms of clindamycin-resistant Streptococcus agalactiae isolates in China |
title_sort | molecular characteristics and antibiotic resistance mechanisms of clindamycin-resistant streptococcus agalactiae isolates in china |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014864/ https://www.ncbi.nlm.nih.gov/pubmed/36937303 http://dx.doi.org/10.3389/fmicb.2023.1138039 |
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