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Bifidobacterium-derived short-chain fatty acids and indole compounds attenuate nonalcoholic fatty liver disease by modulating gut-liver axis

Emerging evidences about gut-microbial modulation have been accumulated in the treatment of nonalcoholic fatty liver disease (NAFLD). We evaluated the effect of Bifidobacterium breve and Bifidobacterium longum on the NAFLD pathology and explore the molecular mechanisms based on multi-omics approache...

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Autores principales: Yoon, Sang Jun, Yu, Jeong Seok, Min, Byeong Hyun, Gupta, Haripriya, Won, Sung-Min, Park, Hee Jin, Han, Sang Hak, Kim, Byung-Yong, Kim, Kyung Hwan, Kim, Byoung Kook, Joung, Hyun Chae, Park, Tae-Sik, Ham, Young Lim, Lee, Do Yup, Suk, Ki Tae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014915/
https://www.ncbi.nlm.nih.gov/pubmed/36937300
http://dx.doi.org/10.3389/fmicb.2023.1129904
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author Yoon, Sang Jun
Yu, Jeong Seok
Min, Byeong Hyun
Gupta, Haripriya
Won, Sung-Min
Park, Hee Jin
Han, Sang Hak
Kim, Byung-Yong
Kim, Kyung Hwan
Kim, Byoung Kook
Joung, Hyun Chae
Park, Tae-Sik
Ham, Young Lim
Lee, Do Yup
Suk, Ki Tae
author_facet Yoon, Sang Jun
Yu, Jeong Seok
Min, Byeong Hyun
Gupta, Haripriya
Won, Sung-Min
Park, Hee Jin
Han, Sang Hak
Kim, Byung-Yong
Kim, Kyung Hwan
Kim, Byoung Kook
Joung, Hyun Chae
Park, Tae-Sik
Ham, Young Lim
Lee, Do Yup
Suk, Ki Tae
author_sort Yoon, Sang Jun
collection PubMed
description Emerging evidences about gut-microbial modulation have been accumulated in the treatment of nonalcoholic fatty liver disease (NAFLD). We evaluated the effect of Bifidobacterium breve and Bifidobacterium longum on the NAFLD pathology and explore the molecular mechanisms based on multi-omics approaches. Human stool analysis [healthy subjects (n = 25) and NAFLD patients (n = 32)] was performed to select NAFLD-associated microbiota. Six-week-old male C57BL/6 J mice were fed a normal chow diet (NC), Western diet (WD), and WD with B. breve (BB) or B. longum (BL; 109 CFU/g) for 8 weeks. Liver/body weight ratio, histopathology, serum/tool analysis, 16S rRNA-sequencing, and metabolites were examined and compared. The BB and BL groups showed improved liver histology and function based on liver/body ratios (WD 7.07 ± 0.75, BB 5.27 ± 0.47, and BL 4.86 ± 0.57) and NAFLD activity scores (WD 5.00 ± 0.10, BB 1.89 ± 1.45, and BL 1.90 ± 0.99; p < 0.05). Strain treatment showed ameliorative effects on gut barrier function. Metagenomic analysis showed treatment-specific changes in taxonomic composition. The community was mainly characterized by the significantly higher composition of the Bacteroidetes phylum among the NC and probiotic-feeding groups. Similarly, the gut metabolome was modulated by probiotics treatment. In particular, short-chain fatty acids and tryptophan metabolites were reverted to normal levels by probiotics, whereas bile acids were partially normalized to those of the NC group. The analysis of gene expression related to lipid and glucose metabolism as well as the immune response indicated the coordinative regulation of β-oxidation, lipogenesis, and systemic inflammation by probiotic treatment. BB and BL attenuate NAFLD by improving microbiome-associated factors of the gut-liver axis.
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spelling pubmed-100149152023-03-16 Bifidobacterium-derived short-chain fatty acids and indole compounds attenuate nonalcoholic fatty liver disease by modulating gut-liver axis Yoon, Sang Jun Yu, Jeong Seok Min, Byeong Hyun Gupta, Haripriya Won, Sung-Min Park, Hee Jin Han, Sang Hak Kim, Byung-Yong Kim, Kyung Hwan Kim, Byoung Kook Joung, Hyun Chae Park, Tae-Sik Ham, Young Lim Lee, Do Yup Suk, Ki Tae Front Microbiol Microbiology Emerging evidences about gut-microbial modulation have been accumulated in the treatment of nonalcoholic fatty liver disease (NAFLD). We evaluated the effect of Bifidobacterium breve and Bifidobacterium longum on the NAFLD pathology and explore the molecular mechanisms based on multi-omics approaches. Human stool analysis [healthy subjects (n = 25) and NAFLD patients (n = 32)] was performed to select NAFLD-associated microbiota. Six-week-old male C57BL/6 J mice were fed a normal chow diet (NC), Western diet (WD), and WD with B. breve (BB) or B. longum (BL; 109 CFU/g) for 8 weeks. Liver/body weight ratio, histopathology, serum/tool analysis, 16S rRNA-sequencing, and metabolites were examined and compared. The BB and BL groups showed improved liver histology and function based on liver/body ratios (WD 7.07 ± 0.75, BB 5.27 ± 0.47, and BL 4.86 ± 0.57) and NAFLD activity scores (WD 5.00 ± 0.10, BB 1.89 ± 1.45, and BL 1.90 ± 0.99; p < 0.05). Strain treatment showed ameliorative effects on gut barrier function. Metagenomic analysis showed treatment-specific changes in taxonomic composition. The community was mainly characterized by the significantly higher composition of the Bacteroidetes phylum among the NC and probiotic-feeding groups. Similarly, the gut metabolome was modulated by probiotics treatment. In particular, short-chain fatty acids and tryptophan metabolites were reverted to normal levels by probiotics, whereas bile acids were partially normalized to those of the NC group. The analysis of gene expression related to lipid and glucose metabolism as well as the immune response indicated the coordinative regulation of β-oxidation, lipogenesis, and systemic inflammation by probiotic treatment. BB and BL attenuate NAFLD by improving microbiome-associated factors of the gut-liver axis. Frontiers Media S.A. 2023-03-01 /pmc/articles/PMC10014915/ /pubmed/36937300 http://dx.doi.org/10.3389/fmicb.2023.1129904 Text en Copyright © 2023 Yoon, Yu, Min, Gupta, Won, Park, Han, Kim, Kim, Kim, Joung, Park, Ham, Lee and Suk. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yoon, Sang Jun
Yu, Jeong Seok
Min, Byeong Hyun
Gupta, Haripriya
Won, Sung-Min
Park, Hee Jin
Han, Sang Hak
Kim, Byung-Yong
Kim, Kyung Hwan
Kim, Byoung Kook
Joung, Hyun Chae
Park, Tae-Sik
Ham, Young Lim
Lee, Do Yup
Suk, Ki Tae
Bifidobacterium-derived short-chain fatty acids and indole compounds attenuate nonalcoholic fatty liver disease by modulating gut-liver axis
title Bifidobacterium-derived short-chain fatty acids and indole compounds attenuate nonalcoholic fatty liver disease by modulating gut-liver axis
title_full Bifidobacterium-derived short-chain fatty acids and indole compounds attenuate nonalcoholic fatty liver disease by modulating gut-liver axis
title_fullStr Bifidobacterium-derived short-chain fatty acids and indole compounds attenuate nonalcoholic fatty liver disease by modulating gut-liver axis
title_full_unstemmed Bifidobacterium-derived short-chain fatty acids and indole compounds attenuate nonalcoholic fatty liver disease by modulating gut-liver axis
title_short Bifidobacterium-derived short-chain fatty acids and indole compounds attenuate nonalcoholic fatty liver disease by modulating gut-liver axis
title_sort bifidobacterium-derived short-chain fatty acids and indole compounds attenuate nonalcoholic fatty liver disease by modulating gut-liver axis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014915/
https://www.ncbi.nlm.nih.gov/pubmed/36937300
http://dx.doi.org/10.3389/fmicb.2023.1129904
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