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Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression
This study aimed to investigate if C21 could prevent acute renal injury induced by sepsis by regulating the expression of p-AKT/PI3K. Five equal groups of 25 adult male Swiss-albino mice were randomly divided (n=5): sham (laparotomy without CLP), CLP, vehicle (equivalent amount of DMSO one hour befo...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Carol Davila University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10015579/ https://www.ncbi.nlm.nih.gov/pubmed/36937479 http://dx.doi.org/10.25122/jml-2022-0299 |
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author | Jabber, Huda Mohammed, Bassim Hadi, Najah Rayish |
author_facet | Jabber, Huda Mohammed, Bassim Hadi, Najah Rayish |
author_sort | Jabber, Huda |
collection | PubMed |
description | This study aimed to investigate if C21 could prevent acute renal injury induced by sepsis by regulating the expression of p-AKT/PI3K. Five equal groups of 25 adult male Swiss-albino mice were randomly divided (n=5): sham (laparotomy without CLP), CLP, vehicle (equivalent amount of DMSO one hour before CLP), and C21 (0.03 mg/kg, one hour before CLP). ELISA was used to measure serum inflammatory mediators, and the expression of PI3K and P-AKT was determined using PCR and immunohistochemistry (IHC), respectively. TNF, TNF receptor, F8-isoprostane, urea, creatinine, and IL-6 blood levels were considerably lower in the CLP group (p<0.05) compared to the sham group, whereas the C21 treated group had significantly (p<0.05) greater levels of these inflammatory mediators. The IHC analysis revealed that P-AKT expression was significantly lower (p<0.05) in the CLP group compared to the sham group, while the C21 pretreatment group had significantly higher levels of P-AKT expression compared to the CLP group (p<0.05). The PI3K expression in the CLP group was significantly lower than in the sham group (p<0.05), according to PCR results, whereas the PI3K expression in the C21 pretreatment group was significantly greater than in the CLP group (p<0.05). This study showed that C21 might reduce levels of pro-inflammatory cytokines, including TNF-, IL-6, and TNF receptor, by modulating the PI3K/AKT signaling pathways, which can, in turn, reduce renal dysfunction during CLP-induced sepsis in male mice. |
format | Online Article Text |
id | pubmed-10015579 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Carol Davila University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-100155792023-03-16 Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression Jabber, Huda Mohammed, Bassim Hadi, Najah Rayish J Med Life Original Article This study aimed to investigate if C21 could prevent acute renal injury induced by sepsis by regulating the expression of p-AKT/PI3K. Five equal groups of 25 adult male Swiss-albino mice were randomly divided (n=5): sham (laparotomy without CLP), CLP, vehicle (equivalent amount of DMSO one hour before CLP), and C21 (0.03 mg/kg, one hour before CLP). ELISA was used to measure serum inflammatory mediators, and the expression of PI3K and P-AKT was determined using PCR and immunohistochemistry (IHC), respectively. TNF, TNF receptor, F8-isoprostane, urea, creatinine, and IL-6 blood levels were considerably lower in the CLP group (p<0.05) compared to the sham group, whereas the C21 treated group had significantly (p<0.05) greater levels of these inflammatory mediators. The IHC analysis revealed that P-AKT expression was significantly lower (p<0.05) in the CLP group compared to the sham group, while the C21 pretreatment group had significantly higher levels of P-AKT expression compared to the CLP group (p<0.05). The PI3K expression in the CLP group was significantly lower than in the sham group (p<0.05), according to PCR results, whereas the PI3K expression in the C21 pretreatment group was significantly greater than in the CLP group (p<0.05). This study showed that C21 might reduce levels of pro-inflammatory cytokines, including TNF-, IL-6, and TNF receptor, by modulating the PI3K/AKT signaling pathways, which can, in turn, reduce renal dysfunction during CLP-induced sepsis in male mice. Carol Davila University Press 2023-02 /pmc/articles/PMC10015579/ /pubmed/36937479 http://dx.doi.org/10.25122/jml-2022-0299 Text en ©2023 JOURNAL of MEDICINE and LIFE https://creativecommons.org/licenses/by/3.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Original Article Jabber, Huda Mohammed, Bassim Hadi, Najah Rayish Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression |
title | Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression |
title_full | Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression |
title_fullStr | Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression |
title_full_unstemmed | Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression |
title_short | Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression |
title_sort | investigating the renoprotective effect of c21 in male mice with sepsis via modulation of p-akt/pi3k expression |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10015579/ https://www.ncbi.nlm.nih.gov/pubmed/36937479 http://dx.doi.org/10.25122/jml-2022-0299 |
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