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Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression

This study aimed to investigate if C21 could prevent acute renal injury induced by sepsis by regulating the expression of p-AKT/PI3K. Five equal groups of 25 adult male Swiss-albino mice were randomly divided (n=5): sham (laparotomy without CLP), CLP, vehicle (equivalent amount of DMSO one hour befo...

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Autores principales: Jabber, Huda, Mohammed, Bassim, Hadi, Najah Rayish
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Carol Davila University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10015579/
https://www.ncbi.nlm.nih.gov/pubmed/36937479
http://dx.doi.org/10.25122/jml-2022-0299
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author Jabber, Huda
Mohammed, Bassim
Hadi, Najah Rayish
author_facet Jabber, Huda
Mohammed, Bassim
Hadi, Najah Rayish
author_sort Jabber, Huda
collection PubMed
description This study aimed to investigate if C21 could prevent acute renal injury induced by sepsis by regulating the expression of p-AKT/PI3K. Five equal groups of 25 adult male Swiss-albino mice were randomly divided (n=5): sham (laparotomy without CLP), CLP, vehicle (equivalent amount of DMSO one hour before CLP), and C21 (0.03 mg/kg, one hour before CLP). ELISA was used to measure serum inflammatory mediators, and the expression of PI3K and P-AKT was determined using PCR and immunohistochemistry (IHC), respectively. TNF, TNF receptor, F8-isoprostane, urea, creatinine, and IL-6 blood levels were considerably lower in the CLP group (p<0.05) compared to the sham group, whereas the C21 treated group had significantly (p<0.05) greater levels of these inflammatory mediators. The IHC analysis revealed that P-AKT expression was significantly lower (p<0.05) in the CLP group compared to the sham group, while the C21 pretreatment group had significantly higher levels of P-AKT expression compared to the CLP group (p<0.05). The PI3K expression in the CLP group was significantly lower than in the sham group (p<0.05), according to PCR results, whereas the PI3K expression in the C21 pretreatment group was significantly greater than in the CLP group (p<0.05). This study showed that C21 might reduce levels of pro-inflammatory cytokines, including TNF-, IL-6, and TNF receptor, by modulating the PI3K/AKT signaling pathways, which can, in turn, reduce renal dysfunction during CLP-induced sepsis in male mice.
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spelling pubmed-100155792023-03-16 Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression Jabber, Huda Mohammed, Bassim Hadi, Najah Rayish J Med Life Original Article This study aimed to investigate if C21 could prevent acute renal injury induced by sepsis by regulating the expression of p-AKT/PI3K. Five equal groups of 25 adult male Swiss-albino mice were randomly divided (n=5): sham (laparotomy without CLP), CLP, vehicle (equivalent amount of DMSO one hour before CLP), and C21 (0.03 mg/kg, one hour before CLP). ELISA was used to measure serum inflammatory mediators, and the expression of PI3K and P-AKT was determined using PCR and immunohistochemistry (IHC), respectively. TNF, TNF receptor, F8-isoprostane, urea, creatinine, and IL-6 blood levels were considerably lower in the CLP group (p<0.05) compared to the sham group, whereas the C21 treated group had significantly (p<0.05) greater levels of these inflammatory mediators. The IHC analysis revealed that P-AKT expression was significantly lower (p<0.05) in the CLP group compared to the sham group, while the C21 pretreatment group had significantly higher levels of P-AKT expression compared to the CLP group (p<0.05). The PI3K expression in the CLP group was significantly lower than in the sham group (p<0.05), according to PCR results, whereas the PI3K expression in the C21 pretreatment group was significantly greater than in the CLP group (p<0.05). This study showed that C21 might reduce levels of pro-inflammatory cytokines, including TNF-, IL-6, and TNF receptor, by modulating the PI3K/AKT signaling pathways, which can, in turn, reduce renal dysfunction during CLP-induced sepsis in male mice. Carol Davila University Press 2023-02 /pmc/articles/PMC10015579/ /pubmed/36937479 http://dx.doi.org/10.25122/jml-2022-0299 Text en ©2023 JOURNAL of MEDICINE and LIFE https://creativecommons.org/licenses/by/3.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Original Article
Jabber, Huda
Mohammed, Bassim
Hadi, Najah Rayish
Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression
title Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression
title_full Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression
title_fullStr Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression
title_full_unstemmed Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression
title_short Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression
title_sort investigating the renoprotective effect of c21 in male mice with sepsis via modulation of p-akt/pi3k expression
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10015579/
https://www.ncbi.nlm.nih.gov/pubmed/36937479
http://dx.doi.org/10.25122/jml-2022-0299
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