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Time-resolved transcriptomic profiling of the developing rabbit’s lungs: impact of premature birth and implications for modelling bronchopulmonary dysplasia

BACKGROUND: Premature birth, perinatal inflammation, and life-saving therapies such as postnatal oxygen and mechanical ventilation are strongly associated with the development of bronchopulmonary dysplasia (BPD); these risk factors, alone or combined, cause lung inflammation and alter programmed mol...

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Autores principales: Storti, Matteo, Faietti, Maria Laura, Murgia, Xabier, Catozzi, Chiara, Minato, Ilaria, Tatoni, Danilo, Cantarella, Simona, Ravanetti, Francesca, Ragionieri, Luisa, Ciccimarra, Roberta, Zoboli, Matteo, Vilanova, Mar, Sánchez-Jiménez, Ester, Gay, Marina, Vilaseca, Marta, Villetti, Gino, Pioselli, Barbara, Salomone, Fabrizio, Ottonello, Simone, Montanini, Barbara, Ricci, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10015812/
https://www.ncbi.nlm.nih.gov/pubmed/36922832
http://dx.doi.org/10.1186/s12931-023-02380-y
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author Storti, Matteo
Faietti, Maria Laura
Murgia, Xabier
Catozzi, Chiara
Minato, Ilaria
Tatoni, Danilo
Cantarella, Simona
Ravanetti, Francesca
Ragionieri, Luisa
Ciccimarra, Roberta
Zoboli, Matteo
Vilanova, Mar
Sánchez-Jiménez, Ester
Gay, Marina
Vilaseca, Marta
Villetti, Gino
Pioselli, Barbara
Salomone, Fabrizio
Ottonello, Simone
Montanini, Barbara
Ricci, Francesca
author_facet Storti, Matteo
Faietti, Maria Laura
Murgia, Xabier
Catozzi, Chiara
Minato, Ilaria
Tatoni, Danilo
Cantarella, Simona
Ravanetti, Francesca
Ragionieri, Luisa
Ciccimarra, Roberta
Zoboli, Matteo
Vilanova, Mar
Sánchez-Jiménez, Ester
Gay, Marina
Vilaseca, Marta
Villetti, Gino
Pioselli, Barbara
Salomone, Fabrizio
Ottonello, Simone
Montanini, Barbara
Ricci, Francesca
author_sort Storti, Matteo
collection PubMed
description BACKGROUND: Premature birth, perinatal inflammation, and life-saving therapies such as postnatal oxygen and mechanical ventilation are strongly associated with the development of bronchopulmonary dysplasia (BPD); these risk factors, alone or combined, cause lung inflammation and alter programmed molecular patterns of normal lung development. The current knowledge on the molecular regulation of lung development mainly derives from mechanistic studies conducted in newborn rodents exposed to postnatal hyperoxia, which have been proven useful but have some limitations. METHODS: Here, we used the rabbit model of BPD as a cost-effective alternative model that mirrors human lung development and, in addition, enables investigating the impact of premature birth per se on the pathophysiology of BPD without further perinatal insults (e.g., hyperoxia, LPS-induced inflammation). First, we characterized the rabbit’s normal lung development along the distinct stages (i.e., pseudoglandular, canalicular, saccular, and alveolar phases) using histological, transcriptomic and proteomic analyses. Then, the impact of premature birth was investigated, comparing the sequential transcriptomic profiles of preterm rabbits obtained at different time intervals during their first week of postnatal life with those from age-matched term pups. RESULTS: Histological findings showed stage-specific morphological features of the developing rabbit’s lung and validated the selected time intervals for the transcriptomic profiling. Cell cycle and embryo development, oxidative phosphorylation, and WNT signaling, among others, showed high gene expression in the pseudoglandular phase. Autophagy, epithelial morphogenesis, response to transforming growth factor β, angiogenesis, epithelium/endothelial cells development, and epithelium/endothelial cells migration pathways appeared upregulated from the 28th day of gestation (early saccular phase), which represents the starting point of the premature rabbit model. Premature birth caused a significant dysregulation of the inflammatory response. TNF-responsive, NF-κB regulated genes were significantly upregulated at premature delivery and triggered downstream inflammatory pathways such as leukocyte activation and cytokine signaling, which persisted upregulated during the first week of life. Preterm birth also dysregulated relevant pathways for normal lung development, such as blood vessel morphogenesis and epithelial-mesenchymal transition. CONCLUSION: These findings establish the 28-day gestation premature rabbit as a suitable model for mechanistic and pharmacological studies in the context of BPD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12931-023-02380-y.
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spelling pubmed-100158122023-03-16 Time-resolved transcriptomic profiling of the developing rabbit’s lungs: impact of premature birth and implications for modelling bronchopulmonary dysplasia Storti, Matteo Faietti, Maria Laura Murgia, Xabier Catozzi, Chiara Minato, Ilaria Tatoni, Danilo Cantarella, Simona Ravanetti, Francesca Ragionieri, Luisa Ciccimarra, Roberta Zoboli, Matteo Vilanova, Mar Sánchez-Jiménez, Ester Gay, Marina Vilaseca, Marta Villetti, Gino Pioselli, Barbara Salomone, Fabrizio Ottonello, Simone Montanini, Barbara Ricci, Francesca Respir Res Research BACKGROUND: Premature birth, perinatal inflammation, and life-saving therapies such as postnatal oxygen and mechanical ventilation are strongly associated with the development of bronchopulmonary dysplasia (BPD); these risk factors, alone or combined, cause lung inflammation and alter programmed molecular patterns of normal lung development. The current knowledge on the molecular regulation of lung development mainly derives from mechanistic studies conducted in newborn rodents exposed to postnatal hyperoxia, which have been proven useful but have some limitations. METHODS: Here, we used the rabbit model of BPD as a cost-effective alternative model that mirrors human lung development and, in addition, enables investigating the impact of premature birth per se on the pathophysiology of BPD without further perinatal insults (e.g., hyperoxia, LPS-induced inflammation). First, we characterized the rabbit’s normal lung development along the distinct stages (i.e., pseudoglandular, canalicular, saccular, and alveolar phases) using histological, transcriptomic and proteomic analyses. Then, the impact of premature birth was investigated, comparing the sequential transcriptomic profiles of preterm rabbits obtained at different time intervals during their first week of postnatal life with those from age-matched term pups. RESULTS: Histological findings showed stage-specific morphological features of the developing rabbit’s lung and validated the selected time intervals for the transcriptomic profiling. Cell cycle and embryo development, oxidative phosphorylation, and WNT signaling, among others, showed high gene expression in the pseudoglandular phase. Autophagy, epithelial morphogenesis, response to transforming growth factor β, angiogenesis, epithelium/endothelial cells development, and epithelium/endothelial cells migration pathways appeared upregulated from the 28th day of gestation (early saccular phase), which represents the starting point of the premature rabbit model. Premature birth caused a significant dysregulation of the inflammatory response. TNF-responsive, NF-κB regulated genes were significantly upregulated at premature delivery and triggered downstream inflammatory pathways such as leukocyte activation and cytokine signaling, which persisted upregulated during the first week of life. Preterm birth also dysregulated relevant pathways for normal lung development, such as blood vessel morphogenesis and epithelial-mesenchymal transition. CONCLUSION: These findings establish the 28-day gestation premature rabbit as a suitable model for mechanistic and pharmacological studies in the context of BPD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12931-023-02380-y. BioMed Central 2023-03-15 2023 /pmc/articles/PMC10015812/ /pubmed/36922832 http://dx.doi.org/10.1186/s12931-023-02380-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Storti, Matteo
Faietti, Maria Laura
Murgia, Xabier
Catozzi, Chiara
Minato, Ilaria
Tatoni, Danilo
Cantarella, Simona
Ravanetti, Francesca
Ragionieri, Luisa
Ciccimarra, Roberta
Zoboli, Matteo
Vilanova, Mar
Sánchez-Jiménez, Ester
Gay, Marina
Vilaseca, Marta
Villetti, Gino
Pioselli, Barbara
Salomone, Fabrizio
Ottonello, Simone
Montanini, Barbara
Ricci, Francesca
Time-resolved transcriptomic profiling of the developing rabbit’s lungs: impact of premature birth and implications for modelling bronchopulmonary dysplasia
title Time-resolved transcriptomic profiling of the developing rabbit’s lungs: impact of premature birth and implications for modelling bronchopulmonary dysplasia
title_full Time-resolved transcriptomic profiling of the developing rabbit’s lungs: impact of premature birth and implications for modelling bronchopulmonary dysplasia
title_fullStr Time-resolved transcriptomic profiling of the developing rabbit’s lungs: impact of premature birth and implications for modelling bronchopulmonary dysplasia
title_full_unstemmed Time-resolved transcriptomic profiling of the developing rabbit’s lungs: impact of premature birth and implications for modelling bronchopulmonary dysplasia
title_short Time-resolved transcriptomic profiling of the developing rabbit’s lungs: impact of premature birth and implications for modelling bronchopulmonary dysplasia
title_sort time-resolved transcriptomic profiling of the developing rabbit’s lungs: impact of premature birth and implications for modelling bronchopulmonary dysplasia
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10015812/
https://www.ncbi.nlm.nih.gov/pubmed/36922832
http://dx.doi.org/10.1186/s12931-023-02380-y
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