A Bidirectional Single‐Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration

Dendritic cell (DC) migration is a fundamental step during execution of its adaptive immunity functions. Studying DC migration characteristics is critical for development of DC‐dependent allergy treatments, vaccines, and cancer immunotherapies. Here, a microfluidics‐based single‐cell migration platf...

Descripción completa

Detalles Bibliográficos
Autores principales: Shao, Ning, Zhou, Yufu, Yao, Jun, Zhang, Pengchao, Song, Yanni, Zhang, Kai, Han, Xin, Wang, Bin, Liu, Xuewu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10015900/
https://www.ncbi.nlm.nih.gov/pubmed/36658690
http://dx.doi.org/10.1002/advs.202204544
_version_ 1784907296492486656
author Shao, Ning
Zhou, Yufu
Yao, Jun
Zhang, Pengchao
Song, Yanni
Zhang, Kai
Han, Xin
Wang, Bin
Liu, Xuewu
author_facet Shao, Ning
Zhou, Yufu
Yao, Jun
Zhang, Pengchao
Song, Yanni
Zhang, Kai
Han, Xin
Wang, Bin
Liu, Xuewu
author_sort Shao, Ning
collection PubMed
description Dendritic cell (DC) migration is a fundamental step during execution of its adaptive immunity functions. Studying DC migration characteristics is critical for development of DC‐dependent allergy treatments, vaccines, and cancer immunotherapies. Here, a microfluidics‐based single‐cell migration platform is described that enables high‐throughput and precise bidirectional cell migration assays. It also allows selective retrieval of cell subpopulations that have different migratory potentials. Using this microfluidic platform, DC migration is investigated in response to different chemoattractants and inhibitors, quantitatively describe DC migration patterns and retrieve DC subpopulations of different migratory potentials for differential gene expression analysis. This platform opens an avenue for precise characterization of cell migration and potential discovery of therapeutic modulators.
format Online
Article
Text
id pubmed-10015900
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-100159002023-03-16 A Bidirectional Single‐Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration Shao, Ning Zhou, Yufu Yao, Jun Zhang, Pengchao Song, Yanni Zhang, Kai Han, Xin Wang, Bin Liu, Xuewu Adv Sci (Weinh) Research Articles Dendritic cell (DC) migration is a fundamental step during execution of its adaptive immunity functions. Studying DC migration characteristics is critical for development of DC‐dependent allergy treatments, vaccines, and cancer immunotherapies. Here, a microfluidics‐based single‐cell migration platform is described that enables high‐throughput and precise bidirectional cell migration assays. It also allows selective retrieval of cell subpopulations that have different migratory potentials. Using this microfluidic platform, DC migration is investigated in response to different chemoattractants and inhibitors, quantitatively describe DC migration patterns and retrieve DC subpopulations of different migratory potentials for differential gene expression analysis. This platform opens an avenue for precise characterization of cell migration and potential discovery of therapeutic modulators. John Wiley and Sons Inc. 2023-01-19 /pmc/articles/PMC10015900/ /pubmed/36658690 http://dx.doi.org/10.1002/advs.202204544 Text en © 2023 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Shao, Ning
Zhou, Yufu
Yao, Jun
Zhang, Pengchao
Song, Yanni
Zhang, Kai
Han, Xin
Wang, Bin
Liu, Xuewu
A Bidirectional Single‐Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration
title A Bidirectional Single‐Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration
title_full A Bidirectional Single‐Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration
title_fullStr A Bidirectional Single‐Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration
title_full_unstemmed A Bidirectional Single‐Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration
title_short A Bidirectional Single‐Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration
title_sort bidirectional single‐cell migration and retrieval chip for quantitative study of dendritic cell migration
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10015900/
https://www.ncbi.nlm.nih.gov/pubmed/36658690
http://dx.doi.org/10.1002/advs.202204544
work_keys_str_mv AT shaoning abidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT zhouyufu abidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT yaojun abidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT zhangpengchao abidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT songyanni abidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT zhangkai abidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT hanxin abidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT wangbin abidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT liuxuewu abidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT shaoning bidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT zhouyufu bidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT yaojun bidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT zhangpengchao bidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT songyanni bidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT zhangkai bidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT hanxin bidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT wangbin bidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration
AT liuxuewu bidirectionalsinglecellmigrationandretrievalchipforquantitativestudyofdendriticcellmigration

Ejemplares similares