Cargando…
Nanosecond pulsed electric field ablates rabbit VX2 liver tumors in a non-thermal manner
BACKGROUND: Liver tumor remains an important cause of cancer-related death. Nanosecond pulsed electric fields (nsPEFs) are advantageous in the treatment of melanoma and pancreatic cancer, but their therapeutic application on liver tumors need to be further studied. METHODS: Hep3B cells were treated...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10016630/ https://www.ncbi.nlm.nih.gov/pubmed/36920938 http://dx.doi.org/10.1371/journal.pone.0273754 |
_version_ | 1784907441772691456 |
---|---|
author | Li, Qing-Gang Liu, Zhen-Guo Dong, Gang Sun, Ying Zou, Ya-Wen Chen, Xiao-Long Wu, Bin Chen, Xin-Hua Ren, Zhi-Gang |
author_facet | Li, Qing-Gang Liu, Zhen-Guo Dong, Gang Sun, Ying Zou, Ya-Wen Chen, Xiao-Long Wu, Bin Chen, Xin-Hua Ren, Zhi-Gang |
author_sort | Li, Qing-Gang |
collection | PubMed |
description | BACKGROUND: Liver tumor remains an important cause of cancer-related death. Nanosecond pulsed electric fields (nsPEFs) are advantageous in the treatment of melanoma and pancreatic cancer, but their therapeutic application on liver tumors need to be further studied. METHODS: Hep3B cells were treated with nsPEFs. The biological behaviors of cells were detected by Cell Counting Kit-8, 5-ethynyl-20-deoxyuridine, and transmission electron microscopy (TEM) assays. In vivo, rabbit VX2 liver tumor models were ablated by ultrasound-guided nsPEFs and radiofrequency ablation (RFA). Contrast-enhanced ultrasound (CEUS) was used to evaluate the ablation effect. HE staining and Masson staining were used to evaluate the tissue morphology after ablation. Immunohistochemistry was performed to determine the expression of Ki67, proliferating cell nuclear antigen, and α-smooth muscle actin at different time points after ablation. RESULTS: The cell viability of Hep3B cells was continuously lower than that of the control group within 3 days after pulse treatment. The proliferation of Hep3B cells was significantly affected by nsPEFs. TEM showed that Hep3B cells underwent significant morphological changes after pulse treatment. In vivo, CEUS imaging showed that nsPEFs could completely ablate model rabbit VX2 liver tumors. After nsPEFs ablation, the area of tumor fibrosis and the expression of Ki67, proliferating cell nuclear antigen, and α-smooth muscle actin were decreased. However, after RFA, rabbit VX2 liver tumor tissue showed complete necrosis, but the expression of PCNA and α-smooth muscle actin did not decrease compared to the tumor group. CONCLUSIONS: nsPEFs can induce Hep3B cells apoptosis and ablate rabbit VX2 liver tumors in a non-thermal manner versus RFA. The ultrasound contrast agent can monitor immediate effect of nsPEF ablation. This study provides a basis for the clinical study of nsPEFs ablation of liver cancer. |
format | Online Article Text |
id | pubmed-10016630 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-100166302023-03-16 Nanosecond pulsed electric field ablates rabbit VX2 liver tumors in a non-thermal manner Li, Qing-Gang Liu, Zhen-Guo Dong, Gang Sun, Ying Zou, Ya-Wen Chen, Xiao-Long Wu, Bin Chen, Xin-Hua Ren, Zhi-Gang PLoS One Research Article BACKGROUND: Liver tumor remains an important cause of cancer-related death. Nanosecond pulsed electric fields (nsPEFs) are advantageous in the treatment of melanoma and pancreatic cancer, but their therapeutic application on liver tumors need to be further studied. METHODS: Hep3B cells were treated with nsPEFs. The biological behaviors of cells were detected by Cell Counting Kit-8, 5-ethynyl-20-deoxyuridine, and transmission electron microscopy (TEM) assays. In vivo, rabbit VX2 liver tumor models were ablated by ultrasound-guided nsPEFs and radiofrequency ablation (RFA). Contrast-enhanced ultrasound (CEUS) was used to evaluate the ablation effect. HE staining and Masson staining were used to evaluate the tissue morphology after ablation. Immunohistochemistry was performed to determine the expression of Ki67, proliferating cell nuclear antigen, and α-smooth muscle actin at different time points after ablation. RESULTS: The cell viability of Hep3B cells was continuously lower than that of the control group within 3 days after pulse treatment. The proliferation of Hep3B cells was significantly affected by nsPEFs. TEM showed that Hep3B cells underwent significant morphological changes after pulse treatment. In vivo, CEUS imaging showed that nsPEFs could completely ablate model rabbit VX2 liver tumors. After nsPEFs ablation, the area of tumor fibrosis and the expression of Ki67, proliferating cell nuclear antigen, and α-smooth muscle actin were decreased. However, after RFA, rabbit VX2 liver tumor tissue showed complete necrosis, but the expression of PCNA and α-smooth muscle actin did not decrease compared to the tumor group. CONCLUSIONS: nsPEFs can induce Hep3B cells apoptosis and ablate rabbit VX2 liver tumors in a non-thermal manner versus RFA. The ultrasound contrast agent can monitor immediate effect of nsPEF ablation. This study provides a basis for the clinical study of nsPEFs ablation of liver cancer. Public Library of Science 2023-03-15 /pmc/articles/PMC10016630/ /pubmed/36920938 http://dx.doi.org/10.1371/journal.pone.0273754 Text en © 2023 Li et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Li, Qing-Gang Liu, Zhen-Guo Dong, Gang Sun, Ying Zou, Ya-Wen Chen, Xiao-Long Wu, Bin Chen, Xin-Hua Ren, Zhi-Gang Nanosecond pulsed electric field ablates rabbit VX2 liver tumors in a non-thermal manner |
title | Nanosecond pulsed electric field ablates rabbit VX2 liver tumors in a non-thermal manner |
title_full | Nanosecond pulsed electric field ablates rabbit VX2 liver tumors in a non-thermal manner |
title_fullStr | Nanosecond pulsed electric field ablates rabbit VX2 liver tumors in a non-thermal manner |
title_full_unstemmed | Nanosecond pulsed electric field ablates rabbit VX2 liver tumors in a non-thermal manner |
title_short | Nanosecond pulsed electric field ablates rabbit VX2 liver tumors in a non-thermal manner |
title_sort | nanosecond pulsed electric field ablates rabbit vx2 liver tumors in a non-thermal manner |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10016630/ https://www.ncbi.nlm.nih.gov/pubmed/36920938 http://dx.doi.org/10.1371/journal.pone.0273754 |
work_keys_str_mv | AT liqinggang nanosecondpulsedelectricfieldablatesrabbitvx2livertumorsinanonthermalmanner AT liuzhenguo nanosecondpulsedelectricfieldablatesrabbitvx2livertumorsinanonthermalmanner AT donggang nanosecondpulsedelectricfieldablatesrabbitvx2livertumorsinanonthermalmanner AT sunying nanosecondpulsedelectricfieldablatesrabbitvx2livertumorsinanonthermalmanner AT zouyawen nanosecondpulsedelectricfieldablatesrabbitvx2livertumorsinanonthermalmanner AT chenxiaolong nanosecondpulsedelectricfieldablatesrabbitvx2livertumorsinanonthermalmanner AT wubin nanosecondpulsedelectricfieldablatesrabbitvx2livertumorsinanonthermalmanner AT chenxinhua nanosecondpulsedelectricfieldablatesrabbitvx2livertumorsinanonthermalmanner AT renzhigang nanosecondpulsedelectricfieldablatesrabbitvx2livertumorsinanonthermalmanner |