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Effects of polyphenols in non-centrifugal cane sugar on saliva secretion: in vitro and in vivo experiments and a randomized controlled trial

This study examined the bioactivities and mechanisms of the non-centrifugal cane sugar polyphenols saponarin, schaftoside, and isoschaftoside in the salivary gland and their effects on salivation. In acute isolated C57BL/6N mouse submandibular gland cells, these polyphenols led to a higher increase...

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Autores principales: Supriya, Shakya, Ushikoshi-Nakayama, Ryoko, Yamazaki, Tomoe, Omagari, Daisuke, Aota, Keiko, Inoue, Hiroko, Matsumoto, Naoyuki, Saito, Ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10017321/
https://www.ncbi.nlm.nih.gov/pubmed/36936876
http://dx.doi.org/10.3164/jcbn.22-114
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author Supriya, Shakya
Ushikoshi-Nakayama, Ryoko
Yamazaki, Tomoe
Omagari, Daisuke
Aota, Keiko
Inoue, Hiroko
Matsumoto, Naoyuki
Saito, Ichiro
author_facet Supriya, Shakya
Ushikoshi-Nakayama, Ryoko
Yamazaki, Tomoe
Omagari, Daisuke
Aota, Keiko
Inoue, Hiroko
Matsumoto, Naoyuki
Saito, Ichiro
author_sort Supriya, Shakya
collection PubMed
description This study examined the bioactivities and mechanisms of the non-centrifugal cane sugar polyphenols saponarin, schaftoside, and isoschaftoside in the salivary gland and their effects on salivation. In acute isolated C57BL/6N mouse submandibular gland cells, these polyphenols led to a higher increase in intracellular calcium after stimulation with the muscarinic agonist carbachol. Stimulation of these cells with polyphenols enhanced ATP production, aquaporin-5 translocation to the plasma membrane and eliminated intracellular reactive oxygen species generated by H(2)O(2). In addition, phosphorylation of endothelial nitric oxide synthase and increased nitric oxide production in vascular endothelial cells were observed. In vivo administration of these polyphenols to C57BL/6N male mice resulted in significantly increased blood flow (saponarin, p = 0.040; isoschaftoside, p = 0.010) and salivation (saponarin, p = 0.031). A randomized controlled trial showed that intake of non-centrifugal cane sugar significantly increased saliva secretion compared with placebo (p = 0.003). These data suggest that non-centrifugal cane sugar polyphenols affect several pathways that support salivation and increase saliva secretion by enhancing vasodilation. Hence, non-centrifugal cane sugar polyphenols can be expected to maintain saliva secretion and improve reduced saliva flow.
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spelling pubmed-100173212023-03-17 Effects of polyphenols in non-centrifugal cane sugar on saliva secretion: in vitro and in vivo experiments and a randomized controlled trial Supriya, Shakya Ushikoshi-Nakayama, Ryoko Yamazaki, Tomoe Omagari, Daisuke Aota, Keiko Inoue, Hiroko Matsumoto, Naoyuki Saito, Ichiro J Clin Biochem Nutr Original Article This study examined the bioactivities and mechanisms of the non-centrifugal cane sugar polyphenols saponarin, schaftoside, and isoschaftoside in the salivary gland and their effects on salivation. In acute isolated C57BL/6N mouse submandibular gland cells, these polyphenols led to a higher increase in intracellular calcium after stimulation with the muscarinic agonist carbachol. Stimulation of these cells with polyphenols enhanced ATP production, aquaporin-5 translocation to the plasma membrane and eliminated intracellular reactive oxygen species generated by H(2)O(2). In addition, phosphorylation of endothelial nitric oxide synthase and increased nitric oxide production in vascular endothelial cells were observed. In vivo administration of these polyphenols to C57BL/6N male mice resulted in significantly increased blood flow (saponarin, p = 0.040; isoschaftoside, p = 0.010) and salivation (saponarin, p = 0.031). A randomized controlled trial showed that intake of non-centrifugal cane sugar significantly increased saliva secretion compared with placebo (p = 0.003). These data suggest that non-centrifugal cane sugar polyphenols affect several pathways that support salivation and increase saliva secretion by enhancing vasodilation. Hence, non-centrifugal cane sugar polyphenols can be expected to maintain saliva secretion and improve reduced saliva flow. the Society for Free Radical Research Japan 2023-03 2022-12-28 /pmc/articles/PMC10017321/ /pubmed/36936876 http://dx.doi.org/10.3164/jcbn.22-114 Text en Copyright © 2023 JCBN https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Supriya, Shakya
Ushikoshi-Nakayama, Ryoko
Yamazaki, Tomoe
Omagari, Daisuke
Aota, Keiko
Inoue, Hiroko
Matsumoto, Naoyuki
Saito, Ichiro
Effects of polyphenols in non-centrifugal cane sugar on saliva secretion: in vitro and in vivo experiments and a randomized controlled trial
title Effects of polyphenols in non-centrifugal cane sugar on saliva secretion: in vitro and in vivo experiments and a randomized controlled trial
title_full Effects of polyphenols in non-centrifugal cane sugar on saliva secretion: in vitro and in vivo experiments and a randomized controlled trial
title_fullStr Effects of polyphenols in non-centrifugal cane sugar on saliva secretion: in vitro and in vivo experiments and a randomized controlled trial
title_full_unstemmed Effects of polyphenols in non-centrifugal cane sugar on saliva secretion: in vitro and in vivo experiments and a randomized controlled trial
title_short Effects of polyphenols in non-centrifugal cane sugar on saliva secretion: in vitro and in vivo experiments and a randomized controlled trial
title_sort effects of polyphenols in non-centrifugal cane sugar on saliva secretion: in vitro and in vivo experiments and a randomized controlled trial
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10017321/
https://www.ncbi.nlm.nih.gov/pubmed/36936876
http://dx.doi.org/10.3164/jcbn.22-114
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