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Consensus design and engineering of an efficient and high-yield peptide asparaginyl ligase for protein cyclization and ligation

Plant legumains are Asn/Asp-specific endopeptidases that have diverse functions in plants. Peptide asparaginyl ligases (PALs) are a special legumain subtype that primarily catalyze peptide bond formation rather than hydrolysis. PALs are versatile protein engineering tools but are rarely found in nat...

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Detalles Bibliográficos
Autores principales:	Hemu, Xinya, Zhang, Xiaohong, Chang, Hong Yi, Poh, Jin En, Tam, James P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Biochemistry and Molecular Biology 2023
Materias:	Research Article Collection: Enzymology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10017362/ https://www.ncbi.nlm.nih.gov/pubmed/36764523 http://dx.doi.org/10.1016/j.jbc.2023.102997

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