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Assembly of nitrogenase biosynthetic pathway in Saccharomyces cerevisiae by using polyprotein strategy

Nitrogenase in some bacteria and archaea catalyzes conversion of N(2) to ammonia. To reconstitute a nitrogenase biosynthetic pathway in a eukaryotic host is still a challenge, since synthesis of nitrogenase requires a large number of nif (nitrogen fixation) genes. Viral 2A peptide mediated “cleavage...

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Detalles Bibliográficos
Autores principales: Wang, Minyang, Shang, Yimin, Liu, Xiaomeng, Chen, Sanfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10017450/
https://www.ncbi.nlm.nih.gov/pubmed/36937264
http://dx.doi.org/10.3389/fmicb.2023.1137355