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Assembly of nitrogenase biosynthetic pathway in Saccharomyces cerevisiae by using polyprotein strategy
Nitrogenase in some bacteria and archaea catalyzes conversion of N(2) to ammonia. To reconstitute a nitrogenase biosynthetic pathway in a eukaryotic host is still a challenge, since synthesis of nitrogenase requires a large number of nif (nitrogen fixation) genes. Viral 2A peptide mediated “cleavage...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10017450/ https://www.ncbi.nlm.nih.gov/pubmed/36937264 http://dx.doi.org/10.3389/fmicb.2023.1137355 |