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Polycystic kidney disease 2-like 1 channel contributes to the bitter aftertaste perception of quinine

Bitterness is an important physiological function in the defense responses to avoid toxic foods. The taste receptor 2 family is well known to mediate bitter taste perception in Type II taste cells. Here, we report that the polycystic kidney disease 2-like 1 (PKD2L1) channel is a novel sensor for the...

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Autores principales: Shimizu, Takahiro, Fujii, Takuto, Hanita, Keisuke, Shinozaki, Ryo, Takamura, Yusaku, Suzuki, Yoshiro, Kageyama, Teppei, Kato, Mizuki, Nishijo, Hisao, Tominaga, Makoto, Sakai, Hideki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10017821/
https://www.ncbi.nlm.nih.gov/pubmed/36922541
http://dx.doi.org/10.1038/s41598-023-31322-3
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author Shimizu, Takahiro
Fujii, Takuto
Hanita, Keisuke
Shinozaki, Ryo
Takamura, Yusaku
Suzuki, Yoshiro
Kageyama, Teppei
Kato, Mizuki
Nishijo, Hisao
Tominaga, Makoto
Sakai, Hideki
author_facet Shimizu, Takahiro
Fujii, Takuto
Hanita, Keisuke
Shinozaki, Ryo
Takamura, Yusaku
Suzuki, Yoshiro
Kageyama, Teppei
Kato, Mizuki
Nishijo, Hisao
Tominaga, Makoto
Sakai, Hideki
author_sort Shimizu, Takahiro
collection PubMed
description Bitterness is an important physiological function in the defense responses to avoid toxic foods. The taste receptor 2 family is well known to mediate bitter taste perception in Type II taste cells. Here, we report that the polycystic kidney disease 2-like 1 (PKD2L1) channel is a novel sensor for the bitter aftertaste in Type III taste cells. The PKD2L1 channel showed rebound activation after the washout of quinine, a bitter tastant, in electrophysiological whole-cell recordings of the PKD2L1-expressing HEK293T cells and Ca(2+)-imaging analysis of Type III taste cells isolated from wild-type PKD2L1 mice. In the short-term two-bottle preference and lick tests in vivo, the wild-type mice avoided normal water while the PKD2L1-knockout mice preferred normal water after they ingested the quinine-containing water. These results may explain the new mechanism of the quinine-triggered bitter aftertaste perception in Type III taste cells.
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spelling pubmed-100178212023-03-17 Polycystic kidney disease 2-like 1 channel contributes to the bitter aftertaste perception of quinine Shimizu, Takahiro Fujii, Takuto Hanita, Keisuke Shinozaki, Ryo Takamura, Yusaku Suzuki, Yoshiro Kageyama, Teppei Kato, Mizuki Nishijo, Hisao Tominaga, Makoto Sakai, Hideki Sci Rep Article Bitterness is an important physiological function in the defense responses to avoid toxic foods. The taste receptor 2 family is well known to mediate bitter taste perception in Type II taste cells. Here, we report that the polycystic kidney disease 2-like 1 (PKD2L1) channel is a novel sensor for the bitter aftertaste in Type III taste cells. The PKD2L1 channel showed rebound activation after the washout of quinine, a bitter tastant, in electrophysiological whole-cell recordings of the PKD2L1-expressing HEK293T cells and Ca(2+)-imaging analysis of Type III taste cells isolated from wild-type PKD2L1 mice. In the short-term two-bottle preference and lick tests in vivo, the wild-type mice avoided normal water while the PKD2L1-knockout mice preferred normal water after they ingested the quinine-containing water. These results may explain the new mechanism of the quinine-triggered bitter aftertaste perception in Type III taste cells. Nature Publishing Group UK 2023-03-15 /pmc/articles/PMC10017821/ /pubmed/36922541 http://dx.doi.org/10.1038/s41598-023-31322-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Shimizu, Takahiro
Fujii, Takuto
Hanita, Keisuke
Shinozaki, Ryo
Takamura, Yusaku
Suzuki, Yoshiro
Kageyama, Teppei
Kato, Mizuki
Nishijo, Hisao
Tominaga, Makoto
Sakai, Hideki
Polycystic kidney disease 2-like 1 channel contributes to the bitter aftertaste perception of quinine
title Polycystic kidney disease 2-like 1 channel contributes to the bitter aftertaste perception of quinine
title_full Polycystic kidney disease 2-like 1 channel contributes to the bitter aftertaste perception of quinine
title_fullStr Polycystic kidney disease 2-like 1 channel contributes to the bitter aftertaste perception of quinine
title_full_unstemmed Polycystic kidney disease 2-like 1 channel contributes to the bitter aftertaste perception of quinine
title_short Polycystic kidney disease 2-like 1 channel contributes to the bitter aftertaste perception of quinine
title_sort polycystic kidney disease 2-like 1 channel contributes to the bitter aftertaste perception of quinine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10017821/
https://www.ncbi.nlm.nih.gov/pubmed/36922541
http://dx.doi.org/10.1038/s41598-023-31322-3
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