Cargando…

Detection and identification of Mucorales and Aspergillus in paraffin-embedded samples by real-time quantitative PCR

BACKGROUND: In this study, we used real-time quantitative PCR (RQ-PCR) to rapidly detect Mucorales and Aspergillus in formalin-fixed, paraffin-embedded (FFPE) samples, targeting 18SrRNA gene and 28SrRNA gene. Identification of Mucorales and Aspergillus was analysed by combining Mucorales RQ-PCR (Muc...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jiang, Xiaolin, Jiang, Yong, Ye, Feng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2023
Materias:	Cellular and Infection Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10017852/ https://www.ncbi.nlm.nih.gov/pubmed/36936757 http://dx.doi.org/10.3389/fcimb.2023.1082347

Ejemplares similares

Blood Mucorales PCR to track down Aspergillus and Mucorales co-infections in at-risk hematology patients: A case-control study
por: Aerts, Robina, et al.
Publicado: (2022)

Genotyping of Aspergillus fumigatus in Formalin-Fixed Paraffin-Embedded Tissues and Serum Samples From Patients With Invasive Aspergillosis
por: de Groot, Theun, et al.
Publicado: (2018)

Unbiased screen for pathogens in human paraffin-embedded tissue samples by whole genome sequencing and metagenomics
por: Nienhold, Ronny, et al.
Publicado: (2022)

Evaluation of environmental Mucorales contamination in and around the residence of COVID-19-associated mucormycosis patients
por: Ghosh, Anup K., et al.
Publicado: (2022)

Detection and quantification of Verticillium dahliae and V. longisporum by droplet digital PCR versus quantitative real-time PCR
por: Wang, Di, et al.
Publicado: (2022)

Rapid detection of Mucorales based on recombinase polymerase amplification and real-time PCR
por: Xu, Rui, et al.
Publicado: (2023)

Rapid and Accurate Identification of SARS-CoV-2 Variants Using Real Time PCR Assays
por: Borillo, Gwynngelle A., et al.
Publicado: (2022)

Developing, Modifying, and Validating a TaqMan Real-Time PCR Technique for Accurate Identification of Leishmania Parasites Causing Most Leishmaniasis in Iran
por: Fotouhi-Ardakani, Reza, et al.
Publicado: (2021)

Development of a Rapid and Fully Automated Multiplex Real-Time PCR Assay for Identification and Differentiation of Vibrio cholerae and Vibrio parahaemolyticus on the BD MAX Platform
por: Li, Zhenpeng, et al.
Publicado: (2021)

Application of Quantitative PCR in the Diagnosis and Evaluating Treatment Efficacy of Leishmaniasis
por: Wu, Yun, et al.
Publicado: (2020)

Design and Evaluation of a Novel Multiplex Real-Time PCR Melting Curve Assay for the Simultaneous Detection of Nine Sexually Transmitted Disease Pathogens in Genitourinary Secretions
por: Hu, Xiao-Mei, et al.
Publicado: (2019)

In Vitro Interactions of Antifungal Agents and Everolimus Against Aspergillus Species
por: Jiang, Huiping, et al.
Publicado: (2022)

Rapid Detection and Differentiating of the Predominant Salmonella Serovars in Chicken Farm by TaqMan Multiplex Real-Time PCR Assay
por: Xin, Suhua, et al.
Publicado: (2021)

Evaluation of Real-Time PCR Coupled With Multiplex Probe Melting Curve Analysis for Pathogen Detection in Patients With Suspected Bloodstream Infections
por: Xiao, Yao, et al.
Publicado: (2019)

Evaluation of the Liberty16 Mobile Real Time PCR Device for Use With the SalivaDirect Assay for SARS-CoV-2 Testing
por: Yolda-Carr, Devyn, et al.
Publicado: (2022)

Detection of macrolide and fluoroquinolone resistance-associated 23S rRNA and parC mutations in Mycoplasma genitalium by nested real-time PCR
por: He, Wenyin, et al.
Publicado: (2023)

Rapid Detection of Aspergillus fumigatus Using Multiple Cross Displacement Amplification Combined With Nanoparticles-Based Lateral Flow
por: Jiang, Luxi, et al.
Publicado: (2021)

Development of a nested PCR assay for specific detection of Metschnikowia bicuspidata infecting Eriocheir sinensis
por: Bao, Jie, et al.
Publicado: (2022)

Species-Specific Immunological Reactivities Depend on the Cell-Wall Organization of the Two Aspergillus, Aspergillus fumigatus and A. flavus
por: Wong, Sarah Sze Wah, et al.
Publicado: (2021)

Polymerase Spiral Reaction Assay for Rapid and Real Time Detection of West Nile Virus From Clinical Samples
por: Tomar, Priyanka Singh, et al.
Publicado: (2020)

What Are the Functions of Chitin Deacetylases in Aspergillus fumigatus?
por: Mouyna, Isabelle, et al.
Publicado: (2020)

Development of a Multiplex PCR Platform for the Rapid Detection of Bacteria, Antibiotic Resistance, and Candida in Human Blood Samples
por: Liotti, Flora Marzia, et al.
Publicado: (2019)

Surveillance diagnostic algorithm using real-time PCR assay and strain typing method development to assist with the control of C. auris amid COVID-19 pandemic
por: Contreras, Deisy A., et al.
Publicado: (2022)

Altered Pseudomonas Strategies to Inhibit Surface Aspergillus Colonies
por: Sass, Gabriele, et al.
Publicado: (2021)

The Direct Semi-Quantitative Detection of 18 Pathogens and Simultaneous Screening for Nine Resistance Genes in Clinical Urine Samples by a High-Throughput Multiplex Genetic Detection System
por: Sun, Zhaoyang, et al.
Publicado: (2021)

Comparison of quantitative real time PCR with Sequencing and ribosomal RNA-FISH for the identification of fungi in Formalin fixed, paraffin-embedded tissue specimens
por: Rickerts, Volker, et al.
Publicado: (2011)

Optimization and analysis of a quantitative real-time PCR-based technique to determine microRNA expression in formalin-fixed paraffin-embedded samples
por: Goswami, Rashmi S, et al.
Publicado: (2010)

Merozoite Proteins Discovered by qRT-PCR-Based Transcriptome Screening of Plasmodium falciparum
por: Hou, Nan, et al.
Publicado: (2021)

Direct Detection and Identification of the Most Common Bacteria and Fungi Causing Otitis Externa by a Stepwise Multiplex PCR
por: Aboutalebian, Shima, et al.
Publicado: (2021)

Detailed characterisation of invasive aspergillosis in a murine model of X-linked chronic granulomatous disease shows new insights in infections caused by Aspergillus fumigatus versus Aspergillus nidulans
por: King, Jill, et al.
Publicado: (2023)

Development and Evaluation of Real-Time Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid and Sensitive Detection of West Nile Virus in Human Clinical Samples
por: Tomar, Priyanka Singh, et al.
Publicado: (2021)

Microsatellite Stability in STR Analysis Aspergillus fumigatus Depends on Number of Repeat Units
por: de Groot, Theun, et al.
Publicado: (2019)

Modulated Response of Aspergillus fumigatus and Stenotrophomonas maltophilia to Antimicrobial Agents in Polymicrobial Biofilm
por: Roisin, Lolita, et al.
Publicado: (2020)

Prospective Evaluation of Aspergillus fumigatus-Specific IgG in Patients With Cystic Fibrosis
por: Eschenhagen, Patience, et al.
Publicado: (2021)

MicroRNA expression in formalin-fixed paraffin embedded tissue using real time quantitative PCR: the strengths and pitfalls
por: Dijkstra, J R, et al.
Publicado: (2012)

Rapid Detection of Enterocytozoon hepatopenaei Infection in Shrimp With a Real-Time Isothermal Recombinase Polymerase Amplification Assay
por: Ma, Chao, et al.
Publicado: (2021)

Multicenter Evaluation of a Novel Immunochromatographic Test for Anti-aspergillus IgG Detection
por: Piarroux, Raphaël P., et al.
Publicado: (2019)

Eosinophil-Associated Innate IL-17 Response Promotes Aspergillus fumigatus Lung Pathology
por: Malacco, Nathália Luísa Sousa de Oliveira, et al.
Publicado: (2019)

Caenorhabditis elegans-Based Aspergillus fumigatus Infection Model for Evaluating Pathogenicity and Drug Efficacy
por: Ahamefule, Chukwuemeka Samson, et al.
Publicado: (2020)

Definition of the Anti-inflammatory Oligosaccharides Derived From the Galactosaminogalactan (GAG) From Aspergillus fumigatus
por: Gressler, Markus, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_pnjcdqp19shggeoh4fuiiufc5n