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Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat

Wheat coleoptile is a sheath-like structure that helps to deliver the first leaf from embryo to the soil surface. Here, a RIL population consisting of 245 lines derived from Zhou 8425B × Chinese Spring cross was genotyped by the high-density Illumina iSelect 90K assay for coleoptile length (CL) QTL...

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Autores principales: Xu, Dengan, Hao, Qianlin, Yang, Tingzhi, Lv, Xinru, Qin, Huimin, Wang, Yalin, Jia, Chenfei, Liu, Wenxing, Dai, Xuehuan, Zeng, Jianbin, Zhang, Hongsheng, He, Zhonghu, Xia, Xianchun, Cao, Shuanghe, Ma, Wujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10017974/
https://www.ncbi.nlm.nih.gov/pubmed/36938052
http://dx.doi.org/10.3389/fpls.2023.1147019
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author Xu, Dengan
Hao, Qianlin
Yang, Tingzhi
Lv, Xinru
Qin, Huimin
Wang, Yalin
Jia, Chenfei
Liu, Wenxing
Dai, Xuehuan
Zeng, Jianbin
Zhang, Hongsheng
He, Zhonghu
Xia, Xianchun
Cao, Shuanghe
Ma, Wujun
author_facet Xu, Dengan
Hao, Qianlin
Yang, Tingzhi
Lv, Xinru
Qin, Huimin
Wang, Yalin
Jia, Chenfei
Liu, Wenxing
Dai, Xuehuan
Zeng, Jianbin
Zhang, Hongsheng
He, Zhonghu
Xia, Xianchun
Cao, Shuanghe
Ma, Wujun
author_sort Xu, Dengan
collection PubMed
description Wheat coleoptile is a sheath-like structure that helps to deliver the first leaf from embryo to the soil surface. Here, a RIL population consisting of 245 lines derived from Zhou 8425B × Chinese Spring cross was genotyped by the high-density Illumina iSelect 90K assay for coleoptile length (CL) QTL mapping. Three QTL for CL were mapped on chromosomes 2BL, 4BS and 4DS. Of them, two major QTL QCL.qau-4BS and QCL.qau-4DS were detected, which could explain 9.1%–22.2% of the phenotypic variances across environments on Rht-B1 and Rht-D1 loci, respectively. Several studies have reported that Rht-B1b may reduce the length of wheat CL but no study has been carried out at molecular level. In order to verify that the Rht-B1 gene is the functional gene for the 4B QTL, an overexpression line Rht-B1b-OE and a CRISPR/SpCas9 line Rht-B1b-KO were studied. The results showed that Rht-B1b overexpression could reduce the CL, while loss-of-function of Rht-B1b would increase the CL relative to that of the null transgenic plants (TNL). To dissect the underlying regulatory mechanism of Rht-B1b on CL, comparative RNA-Seq was conducted between Rht-B1b-OE and TNL. Transcriptome profiles revealed a few key pathways involving the function of Rht-B1b in coleoptile development, including phytohormones, circadian rhythm and starch and sucrose metabolism. Our findings may facilitate wheat breeding for longer coleoptiles to improve seedling early vigor for better penetration through the soil crust in arid regions.
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spelling pubmed-100179742023-03-17 Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat Xu, Dengan Hao, Qianlin Yang, Tingzhi Lv, Xinru Qin, Huimin Wang, Yalin Jia, Chenfei Liu, Wenxing Dai, Xuehuan Zeng, Jianbin Zhang, Hongsheng He, Zhonghu Xia, Xianchun Cao, Shuanghe Ma, Wujun Front Plant Sci Plant Science Wheat coleoptile is a sheath-like structure that helps to deliver the first leaf from embryo to the soil surface. Here, a RIL population consisting of 245 lines derived from Zhou 8425B × Chinese Spring cross was genotyped by the high-density Illumina iSelect 90K assay for coleoptile length (CL) QTL mapping. Three QTL for CL were mapped on chromosomes 2BL, 4BS and 4DS. Of them, two major QTL QCL.qau-4BS and QCL.qau-4DS were detected, which could explain 9.1%–22.2% of the phenotypic variances across environments on Rht-B1 and Rht-D1 loci, respectively. Several studies have reported that Rht-B1b may reduce the length of wheat CL but no study has been carried out at molecular level. In order to verify that the Rht-B1 gene is the functional gene for the 4B QTL, an overexpression line Rht-B1b-OE and a CRISPR/SpCas9 line Rht-B1b-KO were studied. The results showed that Rht-B1b overexpression could reduce the CL, while loss-of-function of Rht-B1b would increase the CL relative to that of the null transgenic plants (TNL). To dissect the underlying regulatory mechanism of Rht-B1b on CL, comparative RNA-Seq was conducted between Rht-B1b-OE and TNL. Transcriptome profiles revealed a few key pathways involving the function of Rht-B1b in coleoptile development, including phytohormones, circadian rhythm and starch and sucrose metabolism. Our findings may facilitate wheat breeding for longer coleoptiles to improve seedling early vigor for better penetration through the soil crust in arid regions. Frontiers Media S.A. 2023-03-02 /pmc/articles/PMC10017974/ /pubmed/36938052 http://dx.doi.org/10.3389/fpls.2023.1147019 Text en Copyright © 2023 Xu, Hao, Yang, Lv, Qin, Wang, Jia, Liu, Dai, Zeng, Zhang, He, Xia, Cao and Ma https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Xu, Dengan
Hao, Qianlin
Yang, Tingzhi
Lv, Xinru
Qin, Huimin
Wang, Yalin
Jia, Chenfei
Liu, Wenxing
Dai, Xuehuan
Zeng, Jianbin
Zhang, Hongsheng
He, Zhonghu
Xia, Xianchun
Cao, Shuanghe
Ma, Wujun
Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat
title Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat
title_full Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat
title_fullStr Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat
title_full_unstemmed Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat
title_short Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat
title_sort impact of “green revolution” gene rht-b1b on coleoptile length of wheat
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10017974/
https://www.ncbi.nlm.nih.gov/pubmed/36938052
http://dx.doi.org/10.3389/fpls.2023.1147019
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