Thiol Quantification Using Colorimetric Thiol–Disulfide Exchange in Nonaqueous Solvents

[Image: see text] A careful analysis of two (thiol–disulfide exchange) thiol quantification chromophores’ behavior (Ellman’s reagent and Aldrithiol-4) in nonaqueous solvents is presented. A wide range of kinetic profiles and response factors were measured to exhibit a large variance for nonaqueous s...

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Autores principales: Schmidt, Ryan, Logan, Matthew G., Patty, Sarah, Ferracane, Jack L., Pfeifer, Carmem S., Kendall, Alexander J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10018523/
https://www.ncbi.nlm.nih.gov/pubmed/36936287
http://dx.doi.org/10.1021/acsomega.2c07792
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author Schmidt, Ryan
Logan, Matthew G.
Patty, Sarah
Ferracane, Jack L.
Pfeifer, Carmem S.
Kendall, Alexander J.
author_facet Schmidt, Ryan
Logan, Matthew G.
Patty, Sarah
Ferracane, Jack L.
Pfeifer, Carmem S.
Kendall, Alexander J.
author_sort Schmidt, Ryan
collection PubMed
description [Image: see text] A careful analysis of two (thiol–disulfide exchange) thiol quantification chromophores’ behavior (Ellman’s reagent and Aldrithiol-4) in nonaqueous solvents is presented. A wide range of kinetic profiles and response factors were measured to exhibit a large variance for nonaqueous systems. We report several robust benchtop and room-temperature methods using different organic solvents compared to aqueous conditions. Validation of analytical analyses in nonaqueous systems and quantification of the cysteine content of ovalbumin are also presented. This work serves as a treatise on the utilization of thiol–disulfide exchange chromophores under nonaqueous conditions for the quantification of thiols.
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spelling pubmed-100185232023-03-17 Thiol Quantification Using Colorimetric Thiol–Disulfide Exchange in Nonaqueous Solvents Schmidt, Ryan Logan, Matthew G. Patty, Sarah Ferracane, Jack L. Pfeifer, Carmem S. Kendall, Alexander J. ACS Omega [Image: see text] A careful analysis of two (thiol–disulfide exchange) thiol quantification chromophores’ behavior (Ellman’s reagent and Aldrithiol-4) in nonaqueous solvents is presented. A wide range of kinetic profiles and response factors were measured to exhibit a large variance for nonaqueous systems. We report several robust benchtop and room-temperature methods using different organic solvents compared to aqueous conditions. Validation of analytical analyses in nonaqueous systems and quantification of the cysteine content of ovalbumin are also presented. This work serves as a treatise on the utilization of thiol–disulfide exchange chromophores under nonaqueous conditions for the quantification of thiols. American Chemical Society 2023-02-28 /pmc/articles/PMC10018523/ /pubmed/36936287 http://dx.doi.org/10.1021/acsomega.2c07792 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Schmidt, Ryan
Logan, Matthew G.
Patty, Sarah
Ferracane, Jack L.
Pfeifer, Carmem S.
Kendall, Alexander J.
Thiol Quantification Using Colorimetric Thiol–Disulfide Exchange in Nonaqueous Solvents
title Thiol Quantification Using Colorimetric Thiol–Disulfide Exchange in Nonaqueous Solvents
title_full Thiol Quantification Using Colorimetric Thiol–Disulfide Exchange in Nonaqueous Solvents
title_fullStr Thiol Quantification Using Colorimetric Thiol–Disulfide Exchange in Nonaqueous Solvents
title_full_unstemmed Thiol Quantification Using Colorimetric Thiol–Disulfide Exchange in Nonaqueous Solvents
title_short Thiol Quantification Using Colorimetric Thiol–Disulfide Exchange in Nonaqueous Solvents
title_sort thiol quantification using colorimetric thiol–disulfide exchange in nonaqueous solvents
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10018523/
https://www.ncbi.nlm.nih.gov/pubmed/36936287
http://dx.doi.org/10.1021/acsomega.2c07792
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