Cargando…

The ALK1-Smad1/5-ID1 pathway participates in tumour angiogenesis induced by low-dose photodynamic therapy

Photodynamic therapy (PDT) is an effective and low-invasive tumour therapy. However, it can induce tumour angiogenesis, which is a main factor leading to tumour recurrence and metastasis. Activin receptor-like kinase-1 (ALK1) is a key factor regulating angiogenesis. However, it remains unclear wheth...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Xiya, Niu, Yajuan, Han, Wang, Han, Xiaoyu, Chen, Qing, Tian, Si, Zhu, Ying, Bai, Dingqun, Li, Kaiting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10019755/
https://www.ncbi.nlm.nih.gov/pubmed/36928315
http://dx.doi.org/10.3892/ijo.2023.5503
_version_ 1784908095108939776
author Guo, Xiya
Niu, Yajuan
Han, Wang
Han, Xiaoyu
Chen, Qing
Tian, Si
Zhu, Ying
Bai, Dingqun
Li, Kaiting
author_facet Guo, Xiya
Niu, Yajuan
Han, Wang
Han, Xiaoyu
Chen, Qing
Tian, Si
Zhu, Ying
Bai, Dingqun
Li, Kaiting
author_sort Guo, Xiya
collection PubMed
description Photodynamic therapy (PDT) is an effective and low-invasive tumour therapy. However, it can induce tumour angiogenesis, which is a main factor leading to tumour recurrence and metastasis. Activin receptor-like kinase-1 (ALK1) is a key factor regulating angiogenesis. However, it remains unclear whether ALK1 plays an unusual role in low-dose PDT-induced tumour angiogenesis. In the present study, human umbilical vein endothelial cells (HUVECs) co-cultured with breast cancer MDA-MB-231 cells (termed HU-231 cells) were used to construct an experimental model of tumour angiogenesis induced by low-dose PDT. The viability, and the proliferative, invasive, migratory, as well as the tube-forming ability of the HU-231 cells were evaluated following low-dose PDT. In particular, ALK1 inhibitor and and an adenovirus against ALK1 were used to further verify the role of ALK1 in low-dose PDT-induced tumour angiogenesis. Moreover, the expression of ALK1, inhibitor of DNA binding 1 (ID1), Smad 1, p-Smad1/5, AKT and PI3K were detected in order to verify the underlying mechanisms. The findings indicated that low-dose PDT enhanced the proliferative ability of the HU-231 cells and reinforced their migratory, invasive and tube formation capacity. However, these effects were reversed with the addition of an ALK1 inhibitor or by the knockdown of ALK1 using adenovirus. These results indicated that ALK1 was involved and played a critical role in tumour angiogenesis induced by low-dose PDT. Furthermore, ALK1 was found to participate in PDT-induced tumour angiogenesis by activating the Smad1/5-ID1 pathway, as opposed to the PI3K/AKT pathway. On the whole, the present study, for the first time, to the best of our knowledge, demonstrates that ALK1 is involved in PDT-induced tumour angiogenesis. The inhibition of ALK1 can suppress PDT-induced tumour angiogenesis, which can enhance the effects of PDT and may thus provide a novel treatment strategy for PDT.
format Online
Article
Text
id pubmed-10019755
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-100197552023-03-17 The ALK1-Smad1/5-ID1 pathway participates in tumour angiogenesis induced by low-dose photodynamic therapy Guo, Xiya Niu, Yajuan Han, Wang Han, Xiaoyu Chen, Qing Tian, Si Zhu, Ying Bai, Dingqun Li, Kaiting Int J Oncol Articles Photodynamic therapy (PDT) is an effective and low-invasive tumour therapy. However, it can induce tumour angiogenesis, which is a main factor leading to tumour recurrence and metastasis. Activin receptor-like kinase-1 (ALK1) is a key factor regulating angiogenesis. However, it remains unclear whether ALK1 plays an unusual role in low-dose PDT-induced tumour angiogenesis. In the present study, human umbilical vein endothelial cells (HUVECs) co-cultured with breast cancer MDA-MB-231 cells (termed HU-231 cells) were used to construct an experimental model of tumour angiogenesis induced by low-dose PDT. The viability, and the proliferative, invasive, migratory, as well as the tube-forming ability of the HU-231 cells were evaluated following low-dose PDT. In particular, ALK1 inhibitor and and an adenovirus against ALK1 were used to further verify the role of ALK1 in low-dose PDT-induced tumour angiogenesis. Moreover, the expression of ALK1, inhibitor of DNA binding 1 (ID1), Smad 1, p-Smad1/5, AKT and PI3K were detected in order to verify the underlying mechanisms. The findings indicated that low-dose PDT enhanced the proliferative ability of the HU-231 cells and reinforced their migratory, invasive and tube formation capacity. However, these effects were reversed with the addition of an ALK1 inhibitor or by the knockdown of ALK1 using adenovirus. These results indicated that ALK1 was involved and played a critical role in tumour angiogenesis induced by low-dose PDT. Furthermore, ALK1 was found to participate in PDT-induced tumour angiogenesis by activating the Smad1/5-ID1 pathway, as opposed to the PI3K/AKT pathway. On the whole, the present study, for the first time, to the best of our knowledge, demonstrates that ALK1 is involved in PDT-induced tumour angiogenesis. The inhibition of ALK1 can suppress PDT-induced tumour angiogenesis, which can enhance the effects of PDT and may thus provide a novel treatment strategy for PDT. D.A. Spandidos 2023-03-15 /pmc/articles/PMC10019755/ /pubmed/36928315 http://dx.doi.org/10.3892/ijo.2023.5503 Text en Copyright: © Guo et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Guo, Xiya
Niu, Yajuan
Han, Wang
Han, Xiaoyu
Chen, Qing
Tian, Si
Zhu, Ying
Bai, Dingqun
Li, Kaiting
The ALK1-Smad1/5-ID1 pathway participates in tumour angiogenesis induced by low-dose photodynamic therapy
title The ALK1-Smad1/5-ID1 pathway participates in tumour angiogenesis induced by low-dose photodynamic therapy
title_full The ALK1-Smad1/5-ID1 pathway participates in tumour angiogenesis induced by low-dose photodynamic therapy
title_fullStr The ALK1-Smad1/5-ID1 pathway participates in tumour angiogenesis induced by low-dose photodynamic therapy
title_full_unstemmed The ALK1-Smad1/5-ID1 pathway participates in tumour angiogenesis induced by low-dose photodynamic therapy
title_short The ALK1-Smad1/5-ID1 pathway participates in tumour angiogenesis induced by low-dose photodynamic therapy
title_sort alk1-smad1/5-id1 pathway participates in tumour angiogenesis induced by low-dose photodynamic therapy
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10019755/
https://www.ncbi.nlm.nih.gov/pubmed/36928315
http://dx.doi.org/10.3892/ijo.2023.5503
work_keys_str_mv AT guoxiya thealk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT niuyajuan thealk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT hanwang thealk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT hanxiaoyu thealk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT chenqing thealk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT tiansi thealk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT zhuying thealk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT baidingqun thealk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT likaiting thealk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT guoxiya alk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT niuyajuan alk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT hanwang alk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT hanxiaoyu alk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT chenqing alk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT tiansi alk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT zhuying alk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT baidingqun alk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy
AT likaiting alk1smad15id1pathwayparticipatesintumourangiogenesisinducedbylowdosephotodynamictherapy