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A Simple Disk Stacking Plus Micro-Elution Method for Rapid Detection of the Synergistic Effect of Aztreonam and Ceftazidime/Avibactam Against Metallo-β-Lactamase Producing Enterobacterales

PURPOSE: To establish and evaluate a simple disk stacking plus micro-elution (DSE) method that can be routinely performed to rapidly detect the synergistic effect between aztreonam (ATM) and ceftazidime/avibactam (CZA) against metallo-β-lactamase (MBL)-producing carbapenem-resistant Enterobacterales...

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Autores principales: Liu, Zhou, Hang, Xiubing, Yan, Tao, Chu, Wenwen, Gong, Zhen, Liu, Yanyan, Dai, Yuanyuan, Yang, Min, Li, Jiabin, Zhou, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10020030/
https://www.ncbi.nlm.nih.gov/pubmed/36937146
http://dx.doi.org/10.2147/IDR.S402275
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author Liu, Zhou
Hang, Xiubing
Yan, Tao
Chu, Wenwen
Gong, Zhen
Liu, Yanyan
Dai, Yuanyuan
Yang, Min
Li, Jiabin
Zhou, Qiang
author_facet Liu, Zhou
Hang, Xiubing
Yan, Tao
Chu, Wenwen
Gong, Zhen
Liu, Yanyan
Dai, Yuanyuan
Yang, Min
Li, Jiabin
Zhou, Qiang
author_sort Liu, Zhou
collection PubMed
description PURPOSE: To establish and evaluate a simple disk stacking plus micro-elution (DSE) method that can be routinely performed to rapidly detect the synergistic effect between aztreonam (ATM) and ceftazidime/avibactam (CZA) against metallo-β-lactamase (MBL)-producing carbapenem-resistant Enterobacterales (CRE). METHODS: The DSE method was established, and a total of 32 MBL-producing CRE isolates collected from multiple centers were tested for ATM-CZA synergy. The results obtained after 8 h of incubation were compared with those obtained by a reference checkerboard assay (CBA) after 18~24 h. Reproducibility experiments were completed on three separate days. RESULTS: The reproducibility study showed that the results of the DSE method were precise. Compared with CBA, the DSE method exhibited excellent performance, with 92.8% sensitivity, 100.0% specificity 93.8% categorical agreement, 0.0% very major error, 0.0% major error, and 6.2% minor error over three days of testing. CONCLUSION: The DSE method is a simple, rapid and practical method for ATM-CZA combination testing. Further evaluation should be completed to improve its clinical application.
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spelling pubmed-100200302023-03-18 A Simple Disk Stacking Plus Micro-Elution Method for Rapid Detection of the Synergistic Effect of Aztreonam and Ceftazidime/Avibactam Against Metallo-β-Lactamase Producing Enterobacterales Liu, Zhou Hang, Xiubing Yan, Tao Chu, Wenwen Gong, Zhen Liu, Yanyan Dai, Yuanyuan Yang, Min Li, Jiabin Zhou, Qiang Infect Drug Resist Short Report PURPOSE: To establish and evaluate a simple disk stacking plus micro-elution (DSE) method that can be routinely performed to rapidly detect the synergistic effect between aztreonam (ATM) and ceftazidime/avibactam (CZA) against metallo-β-lactamase (MBL)-producing carbapenem-resistant Enterobacterales (CRE). METHODS: The DSE method was established, and a total of 32 MBL-producing CRE isolates collected from multiple centers were tested for ATM-CZA synergy. The results obtained after 8 h of incubation were compared with those obtained by a reference checkerboard assay (CBA) after 18~24 h. Reproducibility experiments were completed on three separate days. RESULTS: The reproducibility study showed that the results of the DSE method were precise. Compared with CBA, the DSE method exhibited excellent performance, with 92.8% sensitivity, 100.0% specificity 93.8% categorical agreement, 0.0% very major error, 0.0% major error, and 6.2% minor error over three days of testing. CONCLUSION: The DSE method is a simple, rapid and practical method for ATM-CZA combination testing. Further evaluation should be completed to improve its clinical application. Dove 2023-03-15 /pmc/articles/PMC10020030/ /pubmed/36937146 http://dx.doi.org/10.2147/IDR.S402275 Text en © 2023 Liu et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Short Report
Liu, Zhou
Hang, Xiubing
Yan, Tao
Chu, Wenwen
Gong, Zhen
Liu, Yanyan
Dai, Yuanyuan
Yang, Min
Li, Jiabin
Zhou, Qiang
A Simple Disk Stacking Plus Micro-Elution Method for Rapid Detection of the Synergistic Effect of Aztreonam and Ceftazidime/Avibactam Against Metallo-β-Lactamase Producing Enterobacterales
title A Simple Disk Stacking Plus Micro-Elution Method for Rapid Detection of the Synergistic Effect of Aztreonam and Ceftazidime/Avibactam Against Metallo-β-Lactamase Producing Enterobacterales
title_full A Simple Disk Stacking Plus Micro-Elution Method for Rapid Detection of the Synergistic Effect of Aztreonam and Ceftazidime/Avibactam Against Metallo-β-Lactamase Producing Enterobacterales
title_fullStr A Simple Disk Stacking Plus Micro-Elution Method for Rapid Detection of the Synergistic Effect of Aztreonam and Ceftazidime/Avibactam Against Metallo-β-Lactamase Producing Enterobacterales
title_full_unstemmed A Simple Disk Stacking Plus Micro-Elution Method for Rapid Detection of the Synergistic Effect of Aztreonam and Ceftazidime/Avibactam Against Metallo-β-Lactamase Producing Enterobacterales
title_short A Simple Disk Stacking Plus Micro-Elution Method for Rapid Detection of the Synergistic Effect of Aztreonam and Ceftazidime/Avibactam Against Metallo-β-Lactamase Producing Enterobacterales
title_sort simple disk stacking plus micro-elution method for rapid detection of the synergistic effect of aztreonam and ceftazidime/avibactam against metallo-β-lactamase producing enterobacterales
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10020030/
https://www.ncbi.nlm.nih.gov/pubmed/36937146
http://dx.doi.org/10.2147/IDR.S402275
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