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DNA-based detection of pork content in food

Determination of halal food is essential in ensuring the tranquillity of consumers, especially Muslims. Halal products mean they are free from prohibited ingredients according to Islamic law. One ingredient that is prohibited is food products containing pork and its derivatives. An accurate verifica...

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Detalles Bibliográficos
Autores principales: Muflihah, Hardianto, Ari, Kusumaningtyas, Pintaka, Prabowo, Sulistyo, Hartati, Yeni Wahyuni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10020109/
https://www.ncbi.nlm.nih.gov/pubmed/36938408
http://dx.doi.org/10.1016/j.heliyon.2023.e14418
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author Muflihah
Hardianto, Ari
Kusumaningtyas, Pintaka
Prabowo, Sulistyo
Hartati, Yeni Wahyuni
author_facet Muflihah
Hardianto, Ari
Kusumaningtyas, Pintaka
Prabowo, Sulistyo
Hartati, Yeni Wahyuni
author_sort Muflihah
collection PubMed
description Determination of halal food is essential in ensuring the tranquillity of consumers, especially Muslims. Halal products mean they are free from prohibited ingredients according to Islamic law. One ingredient that is prohibited is food products containing pork and its derivatives. An accurate verification method with a fast result is necessary to meet this requirement for halal food. DNA quantification of pork is now believed to be able to make accurate and quick decisions, as DNA acts as a reservoir or biological characterization of all living things, including pigs, according to specific characteristics of molecular and connection settings. Various DNA-based methods developed include PCR, biosensor and CRISPR methods. This review discussed various DNA-based Keywords: biosensor, CRISPR, detection, DNA, pork, PCR methods, including PCR, biosensor and CRISPR, to detect pork content in food. Among these methods, CRISPR is considered the easiest, fastest and most accurate. Therefore, it is important to develop this method further in the future. In this article, we provide a short review on DNA-based methods for detection of pork content in food products.
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spelling pubmed-100201092023-03-18 DNA-based detection of pork content in food Muflihah Hardianto, Ari Kusumaningtyas, Pintaka Prabowo, Sulistyo Hartati, Yeni Wahyuni Heliyon Review Article Determination of halal food is essential in ensuring the tranquillity of consumers, especially Muslims. Halal products mean they are free from prohibited ingredients according to Islamic law. One ingredient that is prohibited is food products containing pork and its derivatives. An accurate verification method with a fast result is necessary to meet this requirement for halal food. DNA quantification of pork is now believed to be able to make accurate and quick decisions, as DNA acts as a reservoir or biological characterization of all living things, including pigs, according to specific characteristics of molecular and connection settings. Various DNA-based methods developed include PCR, biosensor and CRISPR methods. This review discussed various DNA-based Keywords: biosensor, CRISPR, detection, DNA, pork, PCR methods, including PCR, biosensor and CRISPR, to detect pork content in food. Among these methods, CRISPR is considered the easiest, fastest and most accurate. Therefore, it is important to develop this method further in the future. In this article, we provide a short review on DNA-based methods for detection of pork content in food products. Elsevier 2023-03-08 /pmc/articles/PMC10020109/ /pubmed/36938408 http://dx.doi.org/10.1016/j.heliyon.2023.e14418 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Review Article
Muflihah
Hardianto, Ari
Kusumaningtyas, Pintaka
Prabowo, Sulistyo
Hartati, Yeni Wahyuni
DNA-based detection of pork content in food
title DNA-based detection of pork content in food
title_full DNA-based detection of pork content in food
title_fullStr DNA-based detection of pork content in food
title_full_unstemmed DNA-based detection of pork content in food
title_short DNA-based detection of pork content in food
title_sort dna-based detection of pork content in food
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10020109/
https://www.ncbi.nlm.nih.gov/pubmed/36938408
http://dx.doi.org/10.1016/j.heliyon.2023.e14418
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