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A contractile injection system is required for developmentally regulated cell death in Streptomyces coelicolor

Diverse bacterial species produce extracellular contractile injection systems (eCISs). Although closely related to contractile phage tails, eCISs can inject toxic proteins into eukaryotic cells. Thus, these systems are commonly viewed as cytotoxic defense mechanisms that are not central to other asp...

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Autores principales: Vladimirov, Maria, Zhang, Ruo Xi, Mak, Stefanie, Nodwell, Justin R., Davidson, Alan R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10020575/
https://www.ncbi.nlm.nih.gov/pubmed/36927736
http://dx.doi.org/10.1038/s41467-023-37087-7
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author Vladimirov, Maria
Zhang, Ruo Xi
Mak, Stefanie
Nodwell, Justin R.
Davidson, Alan R.
author_facet Vladimirov, Maria
Zhang, Ruo Xi
Mak, Stefanie
Nodwell, Justin R.
Davidson, Alan R.
author_sort Vladimirov, Maria
collection PubMed
description Diverse bacterial species produce extracellular contractile injection systems (eCISs). Although closely related to contractile phage tails, eCISs can inject toxic proteins into eukaryotic cells. Thus, these systems are commonly viewed as cytotoxic defense mechanisms that are not central to other aspects of bacterial biology. Here, we provide evidence that eCISs appear to participate in the complex developmental process of the bacterium Streptomyces coelicolor. In particular, we show that S. coelicolor produces eCIS particles during its normal growth cycle, and that strains lacking functional eCIS particles exhibit pronounced alterations in their developmental program. Furthermore, eCIS-deficient mutants display reduced levels of cell death and altered morphology during growth in liquid media. Our results suggest that the main role of eCISs in S. coelicolor is to modulate the developmental switch that leads to aerial hyphae formation and sporulation, rather than to attack other species.
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spelling pubmed-100205752023-03-18 A contractile injection system is required for developmentally regulated cell death in Streptomyces coelicolor Vladimirov, Maria Zhang, Ruo Xi Mak, Stefanie Nodwell, Justin R. Davidson, Alan R. Nat Commun Article Diverse bacterial species produce extracellular contractile injection systems (eCISs). Although closely related to contractile phage tails, eCISs can inject toxic proteins into eukaryotic cells. Thus, these systems are commonly viewed as cytotoxic defense mechanisms that are not central to other aspects of bacterial biology. Here, we provide evidence that eCISs appear to participate in the complex developmental process of the bacterium Streptomyces coelicolor. In particular, we show that S. coelicolor produces eCIS particles during its normal growth cycle, and that strains lacking functional eCIS particles exhibit pronounced alterations in their developmental program. Furthermore, eCIS-deficient mutants display reduced levels of cell death and altered morphology during growth in liquid media. Our results suggest that the main role of eCISs in S. coelicolor is to modulate the developmental switch that leads to aerial hyphae formation and sporulation, rather than to attack other species. Nature Publishing Group UK 2023-03-16 /pmc/articles/PMC10020575/ /pubmed/36927736 http://dx.doi.org/10.1038/s41467-023-37087-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Vladimirov, Maria
Zhang, Ruo Xi
Mak, Stefanie
Nodwell, Justin R.
Davidson, Alan R.
A contractile injection system is required for developmentally regulated cell death in Streptomyces coelicolor
title A contractile injection system is required for developmentally regulated cell death in Streptomyces coelicolor
title_full A contractile injection system is required for developmentally regulated cell death in Streptomyces coelicolor
title_fullStr A contractile injection system is required for developmentally regulated cell death in Streptomyces coelicolor
title_full_unstemmed A contractile injection system is required for developmentally regulated cell death in Streptomyces coelicolor
title_short A contractile injection system is required for developmentally regulated cell death in Streptomyces coelicolor
title_sort contractile injection system is required for developmentally regulated cell death in streptomyces coelicolor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10020575/
https://www.ncbi.nlm.nih.gov/pubmed/36927736
http://dx.doi.org/10.1038/s41467-023-37087-7
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