Efficient single copy integration via homology-directed repair (scHDR) by 5′modification of large DNA donor fragments in mice

CRISPR/Cas-based approaches have largely replaced conventional gene targeting strategies. However, homology-directed repair (HDR) in the mouse genome is not very efficient, and precisely inserting longer sequences using HDR remains challenging given that donor constructs preferentially integrate as...

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Detalles Bibliográficos
Autores principales: Medert, Rebekka, Thumberger, Thomas, Tavhelidse-Suck, Tinatini, Hub, Tobias, Kellner, Tanja, Oguchi, Yoko, Dlugosz, Sascha, Zimmermann, Frank, Wittbrodt, Joachim, Freichel, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10021492/
https://www.ncbi.nlm.nih.gov/pubmed/36533445
http://dx.doi.org/10.1093/nar/gkac1150

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