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High seroprevalence of antibodies against SARS-CoV-2 among healthcare workers 8 months after the first wave in Aden, Yemen

The true burden of COVID-19 in Yemen is underestimated. The healthcare system is dysfunctional and there is a high shortage of health care workers in the country. Testing for SARS-CoV-2 remains limited and official surveillance data is restricted to those who are severe or highly suspected. In this...

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Autores principales: Malaeb, Rami, Yousef, Nagwan, Al-Nagdah, Omar, Ali, Qassem Hussein, Saeed, Mohammed Ali Saleh, Haider, Amna, Zelikova, Evgenia, Malou, Nada, Guiramand, Sonia, Mills, Clair, Luquero, Francisco, Porten, Klaudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10022234/
https://www.ncbi.nlm.nih.gov/pubmed/36962647
http://dx.doi.org/10.1371/journal.pgph.0000767
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author Malaeb, Rami
Yousef, Nagwan
Al-Nagdah, Omar
Ali, Qassem Hussein
Saeed, Mohammed Ali Saleh
Haider, Amna
Zelikova, Evgenia
Malou, Nada
Guiramand, Sonia
Mills, Clair
Luquero, Francisco
Porten, Klaudia
author_facet Malaeb, Rami
Yousef, Nagwan
Al-Nagdah, Omar
Ali, Qassem Hussein
Saeed, Mohammed Ali Saleh
Haider, Amna
Zelikova, Evgenia
Malou, Nada
Guiramand, Sonia
Mills, Clair
Luquero, Francisco
Porten, Klaudia
author_sort Malaeb, Rami
collection PubMed
description The true burden of COVID-19 in Yemen is underestimated. The healthcare system is dysfunctional and there is a high shortage of health care workers in the country. Testing for SARS-CoV-2 remains limited and official surveillance data is restricted to those who are severe or highly suspected. In this study, Médecins Sans Frontières (MSF) aimed to conduct serological screening using rapid tests for asymptomatic staff at the MSF Aden Trauma Center to determine the SARS-CoV-2 antibody seropositivity. Four months after the peak of the first wave, we offered all the staff at the MSF Aden Trauma Center PCR if symptomatic, and a baseline SARS-CoV-2 serology screening followed by follow-up screenings. A final round was scheduled four months after the baseline. A rapid serology lateral flow test, NG-Test IgM-IgG was used in all rounds and in the final round, an electrochemiluminescence immunoassay (ECLIA) (Elecsys Anti-SARS-CoV-2 assay). Univariate and multivariate analyses were used to identify risk factors for seropositivity. The level of agreement between the different serology assays used was investigated. Overall 69 out of 356 participants (19.4%, 95% CI 17.9–20.8) tested positive by NG-Test between September and November 2020. A sub-sample of 161 staff members were retested in January 2021. Of these, the NG-Test detected only 13 positive cases, whereas the ECLIA detected 109 positive cases. The adjusted seroprevalence by ECLIA was 59% (95%CI 52.2–65.9). The non-medical staff had significantly lower odds of seropositivity compared to the medical staff (AOR 0.43, 95% CI 0.15–0.7, p<0.001). The positive percent agreement between the two tests was very low (11%). Our results suggest a very high SARS-CoV-2 seroprevalence in healthcare workers in Yemen, highlighting the need for regular testing and rapid vaccination of all healthcare workers in the country.
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spelling pubmed-100222342023-03-17 High seroprevalence of antibodies against SARS-CoV-2 among healthcare workers 8 months after the first wave in Aden, Yemen Malaeb, Rami Yousef, Nagwan Al-Nagdah, Omar Ali, Qassem Hussein Saeed, Mohammed Ali Saleh Haider, Amna Zelikova, Evgenia Malou, Nada Guiramand, Sonia Mills, Clair Luquero, Francisco Porten, Klaudia PLOS Glob Public Health Research Article The true burden of COVID-19 in Yemen is underestimated. The healthcare system is dysfunctional and there is a high shortage of health care workers in the country. Testing for SARS-CoV-2 remains limited and official surveillance data is restricted to those who are severe or highly suspected. In this study, Médecins Sans Frontières (MSF) aimed to conduct serological screening using rapid tests for asymptomatic staff at the MSF Aden Trauma Center to determine the SARS-CoV-2 antibody seropositivity. Four months after the peak of the first wave, we offered all the staff at the MSF Aden Trauma Center PCR if symptomatic, and a baseline SARS-CoV-2 serology screening followed by follow-up screenings. A final round was scheduled four months after the baseline. A rapid serology lateral flow test, NG-Test IgM-IgG was used in all rounds and in the final round, an electrochemiluminescence immunoassay (ECLIA) (Elecsys Anti-SARS-CoV-2 assay). Univariate and multivariate analyses were used to identify risk factors for seropositivity. The level of agreement between the different serology assays used was investigated. Overall 69 out of 356 participants (19.4%, 95% CI 17.9–20.8) tested positive by NG-Test between September and November 2020. A sub-sample of 161 staff members were retested in January 2021. Of these, the NG-Test detected only 13 positive cases, whereas the ECLIA detected 109 positive cases. The adjusted seroprevalence by ECLIA was 59% (95%CI 52.2–65.9). The non-medical staff had significantly lower odds of seropositivity compared to the medical staff (AOR 0.43, 95% CI 0.15–0.7, p<0.001). The positive percent agreement between the two tests was very low (11%). Our results suggest a very high SARS-CoV-2 seroprevalence in healthcare workers in Yemen, highlighting the need for regular testing and rapid vaccination of all healthcare workers in the country. Public Library of Science 2022-11-09 /pmc/articles/PMC10022234/ /pubmed/36962647 http://dx.doi.org/10.1371/journal.pgph.0000767 Text en © 2022 Malaeb et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Malaeb, Rami
Yousef, Nagwan
Al-Nagdah, Omar
Ali, Qassem Hussein
Saeed, Mohammed Ali Saleh
Haider, Amna
Zelikova, Evgenia
Malou, Nada
Guiramand, Sonia
Mills, Clair
Luquero, Francisco
Porten, Klaudia
High seroprevalence of antibodies against SARS-CoV-2 among healthcare workers 8 months after the first wave in Aden, Yemen
title High seroprevalence of antibodies against SARS-CoV-2 among healthcare workers 8 months after the first wave in Aden, Yemen
title_full High seroprevalence of antibodies against SARS-CoV-2 among healthcare workers 8 months after the first wave in Aden, Yemen
title_fullStr High seroprevalence of antibodies against SARS-CoV-2 among healthcare workers 8 months after the first wave in Aden, Yemen
title_full_unstemmed High seroprevalence of antibodies against SARS-CoV-2 among healthcare workers 8 months after the first wave in Aden, Yemen
title_short High seroprevalence of antibodies against SARS-CoV-2 among healthcare workers 8 months after the first wave in Aden, Yemen
title_sort high seroprevalence of antibodies against sars-cov-2 among healthcare workers 8 months after the first wave in aden, yemen
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10022234/
https://www.ncbi.nlm.nih.gov/pubmed/36962647
http://dx.doi.org/10.1371/journal.pgph.0000767
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