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An efficient transformation method for tannin-containing sorghum

BACKGROUND: Tannins are the main bottlenecks restricting the transformation efficiency of plants. Hongyingzi is a special tannin-containing sorghum cultivar used in brewing. METHODS: In this study, a highly efficient microprojectile transformation system for tannin-containing sorghum was successfull...

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Detalles Bibliográficos
Autores principales: Liang, Yuan, Yan, Xuehui, Xu, Jingyi, Liu, Yanlong, Xie, Ke, Li, Jieqin, Zhan, Qiuwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10022505/
https://www.ncbi.nlm.nih.gov/pubmed/36935918
http://dx.doi.org/10.7717/peerj.15066
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author Liang, Yuan
Yan, Xuehui
Xu, Jingyi
Liu, Yanlong
Xie, Ke
Li, Jieqin
Zhan, Qiuwen
author_facet Liang, Yuan
Yan, Xuehui
Xu, Jingyi
Liu, Yanlong
Xie, Ke
Li, Jieqin
Zhan, Qiuwen
author_sort Liang, Yuan
collection PubMed
description BACKGROUND: Tannins are the main bottlenecks restricting the transformation efficiency of plants. Hongyingzi is a special tannin-containing sorghum cultivar used in brewing. METHODS: In this study, a highly efficient microprojectile transformation system for tannin-containing sorghum was successfully exploited using immature embryos (IEs) of Hongyingzi as explants. RESULTS: Hongyingzi presented two types of calli. Type II calli were found to be the most suitable and effective explants for transformation. After optimization of the geneticin (G418) concentration and tissue culture medium, an average transformation frequency of 27% was achieved. Molecular analyzis showed that all transgenic plants were positive and showed transgenes expression. The inheritance analyzis confirmed that the transgenes could be inherited into the next generation. Thus, we successfully established an efficient transformation system for tannin-containing sorghum and demonstrated the possibility of breaking the restriction imposed by tannins in plants.
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spelling pubmed-100225052023-03-18 An efficient transformation method for tannin-containing sorghum Liang, Yuan Yan, Xuehui Xu, Jingyi Liu, Yanlong Xie, Ke Li, Jieqin Zhan, Qiuwen PeerJ Agricultural Science BACKGROUND: Tannins are the main bottlenecks restricting the transformation efficiency of plants. Hongyingzi is a special tannin-containing sorghum cultivar used in brewing. METHODS: In this study, a highly efficient microprojectile transformation system for tannin-containing sorghum was successfully exploited using immature embryos (IEs) of Hongyingzi as explants. RESULTS: Hongyingzi presented two types of calli. Type II calli were found to be the most suitable and effective explants for transformation. After optimization of the geneticin (G418) concentration and tissue culture medium, an average transformation frequency of 27% was achieved. Molecular analyzis showed that all transgenic plants were positive and showed transgenes expression. The inheritance analyzis confirmed that the transgenes could be inherited into the next generation. Thus, we successfully established an efficient transformation system for tannin-containing sorghum and demonstrated the possibility of breaking the restriction imposed by tannins in plants. PeerJ Inc. 2023-03-14 /pmc/articles/PMC10022505/ /pubmed/36935918 http://dx.doi.org/10.7717/peerj.15066 Text en © 2023 Liang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Liang, Yuan
Yan, Xuehui
Xu, Jingyi
Liu, Yanlong
Xie, Ke
Li, Jieqin
Zhan, Qiuwen
An efficient transformation method for tannin-containing sorghum
title An efficient transformation method for tannin-containing sorghum
title_full An efficient transformation method for tannin-containing sorghum
title_fullStr An efficient transformation method for tannin-containing sorghum
title_full_unstemmed An efficient transformation method for tannin-containing sorghum
title_short An efficient transformation method for tannin-containing sorghum
title_sort efficient transformation method for tannin-containing sorghum
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10022505/
https://www.ncbi.nlm.nih.gov/pubmed/36935918
http://dx.doi.org/10.7717/peerj.15066
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