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Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system
Reconstruction of skin equivalents with physiologically relevant cellular and matrix architecture is indispensable for basic research and industrial applications. As skin-nerve crosstalk is increasingly recognized as a major element of skin physiological pathology, the development of reliable in vit...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10023681/ https://www.ncbi.nlm.nih.gov/pubmed/36932093 http://dx.doi.org/10.1038/s41467-023-37187-4 |
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author | Ahn, Jinchul Ohk, Kyungeun Won, Jihee Choi, Dong-Hee Jung, Yong Hun Yang, Ji Hun Jun, Yesl Kim, Jin-A Chung, Seok Lee, Sang-Hoon |
author_facet | Ahn, Jinchul Ohk, Kyungeun Won, Jihee Choi, Dong-Hee Jung, Yong Hun Yang, Ji Hun Jun, Yesl Kim, Jin-A Chung, Seok Lee, Sang-Hoon |
author_sort | Ahn, Jinchul |
collection | PubMed |
description | Reconstruction of skin equivalents with physiologically relevant cellular and matrix architecture is indispensable for basic research and industrial applications. As skin-nerve crosstalk is increasingly recognized as a major element of skin physiological pathology, the development of reliable in vitro models to evaluate the selective communication between epidermal keratinocytes and sensory neurons is being demanded. In this study, we present a three-dimensional innervated epidermal keratinocyte layer as a sensory neuron-epidermal keratinocyte co-culture model on a microfluidic chip using the slope-based air-liquid interfacing culture and spatial compartmentalization. Our co-culture model recapitulates a more organized basal-suprabasal stratification, enhanced barrier function, and physiologically relevant anatomical innervation and demonstrated the feasibility of in situ imaging and functional analysis in a cell-type-specific manner, thereby improving the structural and functional limitations of previous coculture models. This system has the potential as an improved surrogate model and platform for biomedical and pharmaceutical research. |
format | Online Article Text |
id | pubmed-10023681 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-100236812023-03-19 Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system Ahn, Jinchul Ohk, Kyungeun Won, Jihee Choi, Dong-Hee Jung, Yong Hun Yang, Ji Hun Jun, Yesl Kim, Jin-A Chung, Seok Lee, Sang-Hoon Nat Commun Article Reconstruction of skin equivalents with physiologically relevant cellular and matrix architecture is indispensable for basic research and industrial applications. As skin-nerve crosstalk is increasingly recognized as a major element of skin physiological pathology, the development of reliable in vitro models to evaluate the selective communication between epidermal keratinocytes and sensory neurons is being demanded. In this study, we present a three-dimensional innervated epidermal keratinocyte layer as a sensory neuron-epidermal keratinocyte co-culture model on a microfluidic chip using the slope-based air-liquid interfacing culture and spatial compartmentalization. Our co-culture model recapitulates a more organized basal-suprabasal stratification, enhanced barrier function, and physiologically relevant anatomical innervation and demonstrated the feasibility of in situ imaging and functional analysis in a cell-type-specific manner, thereby improving the structural and functional limitations of previous coculture models. This system has the potential as an improved surrogate model and platform for biomedical and pharmaceutical research. Nature Publishing Group UK 2023-03-17 /pmc/articles/PMC10023681/ /pubmed/36932093 http://dx.doi.org/10.1038/s41467-023-37187-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Ahn, Jinchul Ohk, Kyungeun Won, Jihee Choi, Dong-Hee Jung, Yong Hun Yang, Ji Hun Jun, Yesl Kim, Jin-A Chung, Seok Lee, Sang-Hoon Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system |
title | Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system |
title_full | Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system |
title_fullStr | Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system |
title_full_unstemmed | Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system |
title_short | Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system |
title_sort | modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10023681/ https://www.ncbi.nlm.nih.gov/pubmed/36932093 http://dx.doi.org/10.1038/s41467-023-37187-4 |
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