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Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets

Here, we present a cost-effective protocol to differentiate bovine fibro-adipogenic progenitors in a thin hydrogel sheet adherent to 96-well plates. We describe steps for the embedding and culturing of cells in alginate sheets, culture maintenance, and analysis. Compared to alternative three-dimensi...

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Detalles Bibliográficos
Autores principales: Caponi, Joseph J., Shmushkis, Jane, Geissler, Hanna, Post, Mark J., Jackisch, Laura, Mall, Eva M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10024048/
https://www.ncbi.nlm.nih.gov/pubmed/36897775
http://dx.doi.org/10.1016/j.xpro.2023.102143
Descripción
Sumario:Here, we present a cost-effective protocol to differentiate bovine fibro-adipogenic progenitors in a thin hydrogel sheet adherent to 96-well plates. We describe steps for the embedding and culturing of cells in alginate sheets, culture maintenance, and analysis. Compared to alternative three-dimensional (3D) models such as hydrogel-based microfibers, this approach simplifies automation while retaining efficient maturation of adipocytes. Embedded cells are still subjected to a 3D environment, but the sheets can be handled and analyzed like two-dimensional cultures.