Cargando…

Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets

Here, we present a cost-effective protocol to differentiate bovine fibro-adipogenic progenitors in a thin hydrogel sheet adherent to 96-well plates. We describe steps for the embedding and culturing of cells in alginate sheets, culture maintenance, and analysis. Compared to alternative three-dimensi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Caponi, Joseph J., Shmushkis, Jane, Geissler, Hanna, Post, Mark J., Jackisch, Laura, Mall, Eva M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10024048/ https://www.ncbi.nlm.nih.gov/pubmed/36897775 http://dx.doi.org/10.1016/j.xpro.2023.102143

Ejemplares similares

Muscle-derived fibro-adipogenic progenitor cells for production of cultured bovine adipose tissue
por: Dohmen, Richard G. J., et al.
Publicado: (2022)

Fluorescence-activated cell sorting and phenotypic characterization of human fibro-adipogenic progenitors
por: Billeskov, Tine Borum, et al.
Publicado: (2023)

Protocol for photoactivation of YAP in cancer cell spheroids embedded in collagen gels
por: Illes, Bernhard, et al.
Publicado: (2021)

Protocol for electrotaxis of large epithelial cell sheets
por: Zhang, Yan, et al.
Publicado: (2023)

Adipogenic progenitors in different organs: Pathophysiological implications
por: Favaretto, Francesca, et al.
Publicado: (2021)

Protocol for the Design and Assembly of a Light Sheet Light Field Microscope
por: Madrid-Wolff, Jorge, et al.
Publicado: (2019)

Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
por: Ito, Daiyu, et al.
Publicado: (2021)

Protocol for generating embedding-free brain organoids enriched with oligodendrocytes
por: Al-mhanawi, Bahaa, et al.
Publicado: (2023)

Odd skipped-related 1 controls the pro-regenerative response of fibro-adipogenic progenitors
por: Kotsaris, Georgios, et al.
Publicado: (2023)

Chromium Propionate Enhances Adipogenic Differentiation of Bovine Intramuscular Adipocytes
por: Tokach, Rebecca J., et al.
Publicado: (2015)

Adipogenic progenitors keep muscle stem cells young
por: Ancel, Sara, et al.
Publicado: (2019)

MME(+) fibro-adipogenic progenitors are the dominant adipogenic population during fatty infiltration in human skeletal muscle
por: Fitzgerald, Gillian, et al.
Publicado: (2023)

Protocol for production and expression of chimeric bovine-human monoclonal antibodies
por: Heydarchi, Behnaz, et al.
Publicado: (2022)

An updated protocol for in vitro bovine embryo production
por: Speckhart, Savannah L., et al.
Publicado: (2022)

Protocol for constructing a versatile tiling light sheet microscope for imaging cleared tissues
por: Feng, Ruili, et al.
Publicado: (2021)

Role of Metabolic Stress and Exercise in Regulating Fibro/Adipogenic Progenitors
por: Collao, Nicolas, et al.
Publicado: (2020)

Metabolic reprogramming of fibro/adipogenic progenitors facilitates muscle regeneration
por: Reggio, Alessio, et al.
Publicado: (2020)

Janus effect of glucocorticoids on differentiation of muscle fibro/adipogenic progenitors
por: Cerquone Perpetuini, Andrea, et al.
Publicado: (2020)

Two Faces of White Adipose Tissue with Heterogeneous Adipogenic Progenitors
por: Hwang, Injae, et al.
Publicado: (2019)

Fibro-adipogenic progenitors in skeletal muscle homeostasis, regeneration and diseases
por: Molina, Thomas, et al.
Publicado: (2021)

Protocol for isolating adult pituitary stem/progenitor cells in mice
por: Shintani, Aran, et al.
Publicado: (2022)

Senescent fibro-adipogenic progenitors are potential drivers of pathology in inclusion body myositis
por: Nelke, Christopher, et al.
Publicado: (2023)

Immunostaining protocol for infiltrating brain cancer spheroids for light-sheet imaging
por: Bjørknes, Benedicte, et al.
Publicado: (2023)

Activin-dependent signaling in fibro/adipogenic progenitors causes fibrodysplasia ossificans progressiva
por: Lees-Shepard, John B., et al.
Publicado: (2018)

Protocols to generate and isolate mouse myogenic progenitors both in vitro and in vivo
por: Khateb, Mamduh, et al.
Publicado: (2023)

Fibro–Adipogenic Progenitors Cross-Talk in Skeletal Muscle: The Social Network
por: Biferali, Beatrice, et al.
Publicado: (2019)

Fibro-adipogenic progenitors of dystrophic mice are insensitive to NOTCH regulation of adipogenesis
por: Marinkovic, Milica, et al.
Publicado: (2019)

Matrix stiffness and architecture drive fibro-adipogenic progenitors’ activation into myofibroblasts
por: Loomis, Taryn, et al.
Publicado: (2022)

Determinants of epigenetic resistance to HDAC inhibitors in dystrophic fibro‐adipogenic progenitors
por: Consalvi, Silvia, et al.
Publicado: (2022)

Denervation Drives YAP/TAZ Activation in Muscular Fibro/Adipogenic Progenitors
por: Gallardo, Felipe S., et al.
Publicado: (2023)

Protocol to engineer nanofilms embedded lipid nanoparticles for controlled and targeted drug delivery (NECTAR)
por: Porwal, Rashi, et al.
Publicado: (2023)

Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI
por: Smart, Adele, et al.
Publicado: (2023)

Embedding an economist in regional and rural health services to add value and reduce waste by improving local-level decision-making: protocol for the ‘embedded Economist’ program and evaluation
por: Searles, Andrew, et al.
Publicado: (2021)

Zfp423 Promotes Adipogenic Differentiation of Bovine Stromal Vascular Cells
por: Huang, Yan, et al.
Publicado: (2012)

Effect of ciglitazone on adipogenic transdifferentiation of bovine skeletal muscle satellite cells
por: Zhang, Junfang, et al.
Publicado: (2021)

Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
por: Nafria, Monica, et al.
Publicado: (2020)

Protocol for electron microscopy ultrastructural localization of the fusogenic lipid phosphatidic acid on plasma membrane sheets from chromaffin cells
por: Tanguy, Emeline, et al.
Publicado: (2021)

Characterization of adipocytes derived from fibro/adipogenic progenitors resident in human skeletal muscle
por: Arrighi, N, et al.
Publicado: (2015)

Interleukin-15 facilitates muscle regeneration through modulation of fibro/adipogenic progenitors
por: Kang, Xia, et al.
Publicado: (2018)

Dynamics of cellular states of fibro-adipogenic progenitors during myogenesis and muscular dystrophy
por: Malecova, Barbora, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_9436perle5k6cpdm0tae0b8os1