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CREB1 and PPAR-α/γ Pathways in Hepatic Ischemia/Reperfusion: Route for Curcumin to Hepatoprotection

BACKGROUND: Hepatic ischemia/reperfusion injury is a major problem that can exacerbate complications, particularly in liver transplantations. OBJECTIVES: This study aimed to investigate the cellular mechanisms of ischemia/reperfusion injury and hepatoprotection by curcumin. METHODS: Wistar albino ra...

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Autores principales: Demir, Enver Ahmet, Tutuk, Okan, Dogan-Gocmen, Hatice, Ozyilmaz, Duygu Seren, Karagul, Meryem Ilkay, Kara, Mikail, Temiz, Muhyittin, Tumer, Cemil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brieflands 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10024335/
https://www.ncbi.nlm.nih.gov/pubmed/36942070
http://dx.doi.org/10.5812/ijpr-133779
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author Demir, Enver Ahmet
Tutuk, Okan
Dogan-Gocmen, Hatice
Ozyilmaz, Duygu Seren
Karagul, Meryem Ilkay
Kara, Mikail
Temiz, Muhyittin
Tumer, Cemil
author_facet Demir, Enver Ahmet
Tutuk, Okan
Dogan-Gocmen, Hatice
Ozyilmaz, Duygu Seren
Karagul, Meryem Ilkay
Kara, Mikail
Temiz, Muhyittin
Tumer, Cemil
author_sort Demir, Enver Ahmet
collection PubMed
description BACKGROUND: Hepatic ischemia/reperfusion injury is a major problem that can exacerbate complications, particularly in liver transplantations. OBJECTIVES: This study aimed to investigate the cellular mechanisms of ischemia/reperfusion injury and hepatoprotection by curcumin. METHODS: Wistar albino rats were divided into four groups as Control, Sham, I/R, and Cur+I/R. Hepatic ischemia/reperfusion was induced in I/R and Cur+I/R animals, the latter of which was also given 50 mg/kg/day of curcumin for 14 days. Liver aminotransferases and the transcription regulators of inflammation (RelA, IκB, PPAR-α, PPAR-γ, CREB1) were examined along with the histological examination. RESULTS: Hepatic ischemia/reperfusion was found to disrupt hepatic microstructure and downregulate PPAR-α, PPAR-γ, and CREB1 transcripts. Curcumin supplementation in hepatic ischemia/reperfusion recovered the structural organization and promoted the hepatocyte regeneration while increasing expressions of PPARs and CREB1. RelA and IκB were found unaltered, possibly due to the crosstalk between targeted transcripts by ischemia/reperfusion and curcumin. CONCLUSIONS: In sum, PPAR-α/γ and CREB1 were involved in hepatic ischemia/reperfusion and, moreover, were detected to be stimulated by curcumin. PPAR and CREB pathways were found to provide a route to hepatoprotection for curcumin supplementation as evidenced by the microstructural improvement.
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spelling pubmed-100243352023-03-19 CREB1 and PPAR-α/γ Pathways in Hepatic Ischemia/Reperfusion: Route for Curcumin to Hepatoprotection Demir, Enver Ahmet Tutuk, Okan Dogan-Gocmen, Hatice Ozyilmaz, Duygu Seren Karagul, Meryem Ilkay Kara, Mikail Temiz, Muhyittin Tumer, Cemil Iran J Pharm Res Research Article BACKGROUND: Hepatic ischemia/reperfusion injury is a major problem that can exacerbate complications, particularly in liver transplantations. OBJECTIVES: This study aimed to investigate the cellular mechanisms of ischemia/reperfusion injury and hepatoprotection by curcumin. METHODS: Wistar albino rats were divided into four groups as Control, Sham, I/R, and Cur+I/R. Hepatic ischemia/reperfusion was induced in I/R and Cur+I/R animals, the latter of which was also given 50 mg/kg/day of curcumin for 14 days. Liver aminotransferases and the transcription regulators of inflammation (RelA, IκB, PPAR-α, PPAR-γ, CREB1) were examined along with the histological examination. RESULTS: Hepatic ischemia/reperfusion was found to disrupt hepatic microstructure and downregulate PPAR-α, PPAR-γ, and CREB1 transcripts. Curcumin supplementation in hepatic ischemia/reperfusion recovered the structural organization and promoted the hepatocyte regeneration while increasing expressions of PPARs and CREB1. RelA and IκB were found unaltered, possibly due to the crosstalk between targeted transcripts by ischemia/reperfusion and curcumin. CONCLUSIONS: In sum, PPAR-α/γ and CREB1 were involved in hepatic ischemia/reperfusion and, moreover, were detected to be stimulated by curcumin. PPAR and CREB pathways were found to provide a route to hepatoprotection for curcumin supplementation as evidenced by the microstructural improvement. Brieflands 2023-02-01 /pmc/articles/PMC10024335/ /pubmed/36942070 http://dx.doi.org/10.5812/ijpr-133779 Text en Copyright © 2022, Author(s) https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Demir, Enver Ahmet
Tutuk, Okan
Dogan-Gocmen, Hatice
Ozyilmaz, Duygu Seren
Karagul, Meryem Ilkay
Kara, Mikail
Temiz, Muhyittin
Tumer, Cemil
CREB1 and PPAR-α/γ Pathways in Hepatic Ischemia/Reperfusion: Route for Curcumin to Hepatoprotection
title CREB1 and PPAR-α/γ Pathways in Hepatic Ischemia/Reperfusion: Route for Curcumin to Hepatoprotection
title_full CREB1 and PPAR-α/γ Pathways in Hepatic Ischemia/Reperfusion: Route for Curcumin to Hepatoprotection
title_fullStr CREB1 and PPAR-α/γ Pathways in Hepatic Ischemia/Reperfusion: Route for Curcumin to Hepatoprotection
title_full_unstemmed CREB1 and PPAR-α/γ Pathways in Hepatic Ischemia/Reperfusion: Route for Curcumin to Hepatoprotection
title_short CREB1 and PPAR-α/γ Pathways in Hepatic Ischemia/Reperfusion: Route for Curcumin to Hepatoprotection
title_sort creb1 and ppar-α/γ pathways in hepatic ischemia/reperfusion: route for curcumin to hepatoprotection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10024335/
https://www.ncbi.nlm.nih.gov/pubmed/36942070
http://dx.doi.org/10.5812/ijpr-133779
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