Cargando…

Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats

Ovariectomy (OVX) causes a depletion of circulating estradiol (E2) and influences hypothalamic kisspeptin neurons, which govern gonadotropin-releasing hormone (GnRH) release and ultimately gonadotropin secretion. In this study, we examined the changes induced by OVX on the anterior pituitary gland i...

Descripción completa

Detalles Bibliográficos
Autores principales: Oride, Aki, Kanasaki, Haruhiko, Tumurbaatar, Tuvshintugs, Tumurgan, Zolzaya, Okada, Hiroe, Cairang, Zhuoma, Satoru, Kyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10024625/
https://www.ncbi.nlm.nih.gov/pubmed/36941851
http://dx.doi.org/10.1155/2023/3143347
_version_ 1784909148056453120
author Oride, Aki
Kanasaki, Haruhiko
Tumurbaatar, Tuvshintugs
Tumurgan, Zolzaya
Okada, Hiroe
Cairang, Zhuoma
Satoru, Kyo
author_facet Oride, Aki
Kanasaki, Haruhiko
Tumurbaatar, Tuvshintugs
Tumurgan, Zolzaya
Okada, Hiroe
Cairang, Zhuoma
Satoru, Kyo
author_sort Oride, Aki
collection PubMed
description Ovariectomy (OVX) causes a depletion of circulating estradiol (E2) and influences hypothalamic kisspeptin neurons, which govern gonadotropin-releasing hormone (GnRH) release and ultimately gonadotropin secretion. In this study, we examined the changes induced by OVX on the anterior pituitary gland in female rats. OVX significantly increased the mRNA expression of gonadotropin α, luteinizing hormone (LH) β, and follicle-stimulating hormone (FSH) β subunits within the pituitary gland compared with control (sham-operated) rats, and this was completely suppressed by E2 supplementation. High-dose dihydrotestosterone supplementation also prevented the OVX-induced increase in the expression of the three gonadotropin subunits. GnRH receptor mRNA expression within the pituitary was significantly increased in OVX rats, and this increase was completely inhibited by E2 supplementation. The mRNA expression of the receptors for adenylate cyclase-activating polypeptide and kisspeptin was unchanged by OVX. Although the mRNA levels of inhibin α, βA, and βB subunits within the pituitary gland were not modulated by OVX, follistatin gene expression within the pituitary gland was increased by OVX, and this increase was completely inhibited by E2 supplementation after OVX. In experiments using a pituitary gonadotroph cell model (LβT2 cells), follistatin itself did not modulate the mRNA expression of gonadotropin LHβ and FSHβ subunits, and the GnRH-induced increase in the expression of these genes was slightly inhibited in the presence of follistatin. Our current observations suggest that OVX induces several characteristic changes in the pituitary gland of rats.
format Online
Article
Text
id pubmed-10024625
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-100246252023-03-19 Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats Oride, Aki Kanasaki, Haruhiko Tumurbaatar, Tuvshintugs Tumurgan, Zolzaya Okada, Hiroe Cairang, Zhuoma Satoru, Kyo Int J Endocrinol Research Article Ovariectomy (OVX) causes a depletion of circulating estradiol (E2) and influences hypothalamic kisspeptin neurons, which govern gonadotropin-releasing hormone (GnRH) release and ultimately gonadotropin secretion. In this study, we examined the changes induced by OVX on the anterior pituitary gland in female rats. OVX significantly increased the mRNA expression of gonadotropin α, luteinizing hormone (LH) β, and follicle-stimulating hormone (FSH) β subunits within the pituitary gland compared with control (sham-operated) rats, and this was completely suppressed by E2 supplementation. High-dose dihydrotestosterone supplementation also prevented the OVX-induced increase in the expression of the three gonadotropin subunits. GnRH receptor mRNA expression within the pituitary was significantly increased in OVX rats, and this increase was completely inhibited by E2 supplementation. The mRNA expression of the receptors for adenylate cyclase-activating polypeptide and kisspeptin was unchanged by OVX. Although the mRNA levels of inhibin α, βA, and βB subunits within the pituitary gland were not modulated by OVX, follistatin gene expression within the pituitary gland was increased by OVX, and this increase was completely inhibited by E2 supplementation after OVX. In experiments using a pituitary gonadotroph cell model (LβT2 cells), follistatin itself did not modulate the mRNA expression of gonadotropin LHβ and FSHβ subunits, and the GnRH-induced increase in the expression of these genes was slightly inhibited in the presence of follistatin. Our current observations suggest that OVX induces several characteristic changes in the pituitary gland of rats. Hindawi 2023-03-11 /pmc/articles/PMC10024625/ /pubmed/36941851 http://dx.doi.org/10.1155/2023/3143347 Text en Copyright © 2023 Aki Oride et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Oride, Aki
Kanasaki, Haruhiko
Tumurbaatar, Tuvshintugs
Tumurgan, Zolzaya
Okada, Hiroe
Cairang, Zhuoma
Satoru, Kyo
Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats
title Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats
title_full Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats
title_fullStr Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats
title_full_unstemmed Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats
title_short Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats
title_sort impact of ovariectomy on the anterior pituitary gland in female rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10024625/
https://www.ncbi.nlm.nih.gov/pubmed/36941851
http://dx.doi.org/10.1155/2023/3143347
work_keys_str_mv AT orideaki impactofovariectomyontheanteriorpituitaryglandinfemalerats
AT kanasakiharuhiko impactofovariectomyontheanteriorpituitaryglandinfemalerats
AT tumurbaatartuvshintugs impactofovariectomyontheanteriorpituitaryglandinfemalerats
AT tumurganzolzaya impactofovariectomyontheanteriorpituitaryglandinfemalerats
AT okadahiroe impactofovariectomyontheanteriorpituitaryglandinfemalerats
AT cairangzhuoma impactofovariectomyontheanteriorpituitaryglandinfemalerats
AT satorukyo impactofovariectomyontheanteriorpituitaryglandinfemalerats