Cargando…

Thin film notch filters as platforms for biological image processing

Many image processing operations involve the modification of the spatial frequency content of images. Here we demonstrate object-plane spatial frequency filtering utilizing the angular sensitivity of a commercial spectral bandstop filter. This approach to all-optical image processing is shown to gen...

Descripción completa

Detalles Bibliográficos
Autores principales: Sulejman, Shaban B., Priscilla, Niken, Wesemann, Lukas, Lee, Wendy S. L., Lou, Jieqiong, Hinde, Elizabeth, Davis, Timothy J., Roberts, Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10024701/
https://www.ncbi.nlm.nih.gov/pubmed/36934126
http://dx.doi.org/10.1038/s41598-023-31528-5
_version_ 1784909164971032576
author Sulejman, Shaban B.
Priscilla, Niken
Wesemann, Lukas
Lee, Wendy S. L.
Lou, Jieqiong
Hinde, Elizabeth
Davis, Timothy J.
Roberts, Ann
author_facet Sulejman, Shaban B.
Priscilla, Niken
Wesemann, Lukas
Lee, Wendy S. L.
Lou, Jieqiong
Hinde, Elizabeth
Davis, Timothy J.
Roberts, Ann
author_sort Sulejman, Shaban B.
collection PubMed
description Many image processing operations involve the modification of the spatial frequency content of images. Here we demonstrate object-plane spatial frequency filtering utilizing the angular sensitivity of a commercial spectral bandstop filter. This approach to all-optical image processing is shown to generate real-time pseudo-3D images of transparent biological and other samples, such as human cervical cancer cells. This work demonstrates the potential of non-local, non-interferometric approaches to image processing for uses in label-free biological cell imaging and dynamical monitoring.
format Online
Article
Text
id pubmed-10024701
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-100247012023-03-20 Thin film notch filters as platforms for biological image processing Sulejman, Shaban B. Priscilla, Niken Wesemann, Lukas Lee, Wendy S. L. Lou, Jieqiong Hinde, Elizabeth Davis, Timothy J. Roberts, Ann Sci Rep Article Many image processing operations involve the modification of the spatial frequency content of images. Here we demonstrate object-plane spatial frequency filtering utilizing the angular sensitivity of a commercial spectral bandstop filter. This approach to all-optical image processing is shown to generate real-time pseudo-3D images of transparent biological and other samples, such as human cervical cancer cells. This work demonstrates the potential of non-local, non-interferometric approaches to image processing for uses in label-free biological cell imaging and dynamical monitoring. Nature Publishing Group UK 2023-03-18 /pmc/articles/PMC10024701/ /pubmed/36934126 http://dx.doi.org/10.1038/s41598-023-31528-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Sulejman, Shaban B.
Priscilla, Niken
Wesemann, Lukas
Lee, Wendy S. L.
Lou, Jieqiong
Hinde, Elizabeth
Davis, Timothy J.
Roberts, Ann
Thin film notch filters as platforms for biological image processing
title Thin film notch filters as platforms for biological image processing
title_full Thin film notch filters as platforms for biological image processing
title_fullStr Thin film notch filters as platforms for biological image processing
title_full_unstemmed Thin film notch filters as platforms for biological image processing
title_short Thin film notch filters as platforms for biological image processing
title_sort thin film notch filters as platforms for biological image processing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10024701/
https://www.ncbi.nlm.nih.gov/pubmed/36934126
http://dx.doi.org/10.1038/s41598-023-31528-5
work_keys_str_mv AT sulejmanshabanb thinfilmnotchfiltersasplatformsforbiologicalimageprocessing
AT priscillaniken thinfilmnotchfiltersasplatformsforbiologicalimageprocessing
AT wesemannlukas thinfilmnotchfiltersasplatformsforbiologicalimageprocessing
AT leewendysl thinfilmnotchfiltersasplatformsforbiologicalimageprocessing
AT loujieqiong thinfilmnotchfiltersasplatformsforbiologicalimageprocessing
AT hindeelizabeth thinfilmnotchfiltersasplatformsforbiologicalimageprocessing
AT davistimothyj thinfilmnotchfiltersasplatformsforbiologicalimageprocessing
AT robertsann thinfilmnotchfiltersasplatformsforbiologicalimageprocessing