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Protocol for genome-scale in vivo CRISPR screening to study protection of beta cells under autoimmunity in a type 1 diabetes mouse model

Autoimmunity-induced pancreatic beta cell failure is the main characteristic of type 1 diabetes (T1D). Here, we describe a protocol for genome-scale in vivo CRISPR-Cas9 screening for use in a mouse model of T1D. Using a non-obese-diabetic-derived mouse beta cell line, NIT-1, and a genome-wide CRISPR...

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Detalles Bibliográficos
Autores principales:	Li, Jian, Lee, Yu-Chi, Iessi, Isabela L., Wu, Chialing, Yi, Peng, Cai, Erica P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10025263/ https://www.ncbi.nlm.nih.gov/pubmed/36917606 http://dx.doi.org/10.1016/j.xpro.2023.102155

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