Protocol to engineer apical-out airway organoids using suspension culture of human airway basal stem cell aggregates

Here we present a protocol to engineer apical-out airway organoids (AOAOs) directly from human airway basal stem cells (hABSCs) using suspension culture of hABSC aggregates on a cell-repellent surface. We describe steps to produce spherical AOAOs with homogenous presentation of exterior-facing motil...

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Detalles Bibliográficos
Autores principales: Wijesekara, Piyumi, Patel, Kian Z., Otto, Ellen L., Campbell, Phil G., Ren, Xi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10025264/
https://www.ncbi.nlm.nih.gov/pubmed/36917607
http://dx.doi.org/10.1016/j.xpro.2023.102154

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