Optimized protocol for analysis of neural stem proliferation in human-pluripotent-stem-cell-derived cerebral organoids

Cerebral organoids represent an optimal experimental system for studying human cortical development, evolution, physiology, function, and disease mechanisms. Here, we describe a simple protocol for the differentiation of human pluripotent stem cells (hPSCs) into cerebral organoids. We describe steps for hPSC maintenance, neural induction of embryoid bodies, and patterning of cerebral organoids. We also detail a process for the phenotypic assay of each neural-tube-like area in hPSC-derived cerebral organoids. For complete details on the use and execution of this protocol, please refer to Tang et al. (2021).(1)