Cargando…

Optimized protocol for analysis of neural stem proliferation in human-pluripotent-stem-cell-derived cerebral organoids

Cerebral organoids represent an optimal experimental system for studying human cortical development, evolution, physiology, function, and disease mechanisms. Here, we describe a simple protocol for the differentiation of human pluripotent stem cells (hPSCs) into cerebral organoids. We describe steps...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tang, Xiao-Yan, Wang, Da, Zhang, Xin-Yue, Xu, Min, Liu, Yan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026034/ https://www.ncbi.nlm.nih.gov/pubmed/36924505 http://dx.doi.org/10.1016/j.xpro.2023.102169

Ejemplares similares

Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell-derived cerebral organoids
por: Feng, Yilin, et al.
Publicado: (2023)

Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization
por: Anastasaki, Corina, et al.
Publicado: (2022)

Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin
por: Kim, Hyosung, et al.
Publicado: (2021)

Protocol for SARS-CoV-2 infection of kidney organoids derived from human pluripotent stem cells
por: Garreta, Elena, et al.
Publicado: (2022)

Protocol for Large-Scale Production of Kidney Organoids from Human Pluripotent Stem Cells
por: Sander, Veronika, et al.
Publicado: (2020)

Optimized protocol for naive human pluripotent stem cell-derived trophoblast induction
por: Io, Shingo, et al.
Publicado: (2021)

Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells
por: Ishikura, Yukiko, et al.
Publicado: (2022)

Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96
por: Miguel, Verónica, et al.
Publicado: (2023)

Differentiation of human pluripotent stem cells into pancreatic duct-like organoids
por: Breunig, Markus, et al.
Publicado: (2021)

Differentiation and single-cell RNA-seq analyses of human pluripotent-stem-cell-derived renal organoids
por: Lian, Eric, et al.
Publicado: (2023)

Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons
por: Sheta, Razan, et al.
Publicado: (2023)

Protocol to derive human trophoblast stem cells directly from primed pluripotent stem cells
por: Wei, Yanxing, et al.
Publicado: (2022)

Modeling the Effects of Maternal Diabetes on the Developing Human Heart Using Pluripotent Stem Cell–Derived Heart Organoids
por: Lewis‐Israeli, Yonatan R., et al.
Publicado: (2022)

Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids
por: Pedraza-Arevalo, Sergio, et al.
Publicado: (2022)

Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media
por: Kameda, Kotaro, et al.
Publicado: (2022)

The Use of Pluripotent Stem Cell-Derived Organoids to Study Extracellular Matrix Development during Neural Degeneration
por: Yan, Yuanwei, et al.
Publicado: (2019)

Protocol to optimize the biobanking of ovarian cancer organoids by accommodating patient-specific differences in stemness potential
por: Trillsch, Fabian, et al.
Publicado: (2023)

HIPRO: A High-Efficiency, Hypoxia-Induced Protocol for Generation of Photoreceptors in Retinal Organoids from Mouse Pluripotent Stem Cells
por: Chen, Holly Y., et al.
Publicado: (2020)

Accelerated Development of Rod Photoreceptors in Retinal Organoids Derived from Human Pluripotent Stem Cells by Supplementation with 9-cis Retinal
por: Kelley, Ryan A., et al.
Publicado: (2020)

Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
por: Mucci, Sofía, et al.
Publicado: (2022)

Challenges for the Applications of Human Pluripotent Stem Cell-Derived Liver Organoids
por: Chang, Mingyang, et al.
Publicado: (2021)

Gene Editing in Pluripotent Stem Cells and Their Derived Organoids
por: Zhou, Hang, et al.
Publicado: (2021)

Generation of human embryonic stem cell-derived lung organoids
por: Han, Le, et al.
Publicado: (2022)

High-throughput generation of midbrain dopaminergic neuron organoids from reporter human pluripotent stem cells
por: Sarrafha, Lily, et al.
Publicado: (2021)

An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells
por: Haque, Mohammad, et al.
Publicado: (2021)

Pluripotent Stem Cell-Derived Cerebral Organoids Reveal Human Oligodendrogenesis with Dorsal and Ventral Origins
por: Kim, Hyosung, et al.
Publicado: (2019)

Human‐induced pluripotent stem cell‐derived cerebral organoid of leukoencephalopathy with vanishing white matter
por: Deng, Jiong, et al.
Publicado: (2023)

Scalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors
por: Pollock, Samuel D., et al.
Publicado: (2023)

High-content screening of mitochondrial polarization in neural cells derived from human pluripotent stem cells
por: Zink, Annika, et al.
Publicado: (2022)

Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons
por: Yin, Xiling, et al.
Publicado: (2021)

A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes
por: Knight, Walter E., et al.
Publicado: (2021)

Design Principles for Pluripotent Stem Cell-Derived Organoid Engineering
por: Silva, Teresa P., et al.
Publicado: (2019)

Human Pluripotent Stem Cell-Derived Alveolar Organoid with Macrophages
por: Seo, Ha-Rim, et al.
Publicado: (2022)

Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells
por: Ciarpella, Francesca, et al.
Publicado: (2023)

Modeling alcohol-induced neurotoxicity using human induced pluripotent stem cell-derived three-dimensional cerebral organoids
por: Arzua, Thiago, et al.
Publicado: (2020)

Generating Patient-Derived Gliomas within Cerebral Organoids
por: Linkous, Amanda, et al.
Publicado: (2020)

Cerebral organoids derived from Sandhoff disease-induced pluripotent stem cells exhibit impaired neurodifferentiation
por: Allende, Maria L., et al.
Publicado: (2018)

A simple method to improve the quality and yield of human pluripotent stem cell-derived cerebral organoids
por: Choe, Mu Seog, et al.
Publicado: (2021)

Microglia-containing cerebral organoids derived from induced pluripotent stem cells for the study of neurological diseases
por: Hong, Yiling, et al.
Publicado: (2023)

Modeling human retinoblastoma using embryonic stem cell-derived retinal organoids
por: Liu, Hui, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_q8t4ila9decrukrqej5c75bq7j