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Electrostatic Theory of the Acidity of the Solution in the Lumina of Viruses and Virus-Like Particles
[Image: see text] Recently, Maassen et al. measured an appreciable pH difference between the bulk solution and the solution in the lumen of virus-like particles, self-assembled in an aqueous buffer solution containing the coat proteins of a simple plant virus and polyanions (Maassen, S. J.; et al. S...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026070/ https://www.ncbi.nlm.nih.gov/pubmed/36881522 http://dx.doi.org/10.1021/acs.jpcb.2c08604 |
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author | Muhren, H. J. van der Schoot, Paul |
author_facet | Muhren, H. J. van der Schoot, Paul |
author_sort | Muhren, H. J. |
collection | PubMed |
description | [Image: see text] Recently, Maassen et al. measured an appreciable pH difference between the bulk solution and the solution in the lumen of virus-like particles, self-assembled in an aqueous buffer solution containing the coat proteins of a simple plant virus and polyanions (Maassen, S. J.; et al. Small2018, 14, 1802081). They attribute this to the Donnan effect, caused by an imbalance between the number of negative charges on the encapsulated polyelectrolyte molecules and the number of positive charges on the RNA binding domains of the coat proteins that make up the virus shell or capsid. By applying Poisson–Boltzmann theory, we confirm this conclusion and show that simple Donnan theory is accurate even for the smallest of viruses and virus-like particles. This, in part, is due to the additional screening caused by the presence of a large number of immobile charges in the cavity of the shell. The presence of a net charge on the outer surface of the capsid we find in practice to not have a large effect on the pH shift. Hence, Donnan theory can indeed be applied to connect the local pH and the amount of encapsulated material. The large shifts up to a full pH unit that we predict must have consequences for applications of virus capsids as nanocontainers in bionanotechnology and artificial cell organelles. |
format | Online Article Text |
id | pubmed-10026070 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-100260702023-03-21 Electrostatic Theory of the Acidity of the Solution in the Lumina of Viruses and Virus-Like Particles Muhren, H. J. van der Schoot, Paul J Phys Chem B [Image: see text] Recently, Maassen et al. measured an appreciable pH difference between the bulk solution and the solution in the lumen of virus-like particles, self-assembled in an aqueous buffer solution containing the coat proteins of a simple plant virus and polyanions (Maassen, S. J.; et al. Small2018, 14, 1802081). They attribute this to the Donnan effect, caused by an imbalance between the number of negative charges on the encapsulated polyelectrolyte molecules and the number of positive charges on the RNA binding domains of the coat proteins that make up the virus shell or capsid. By applying Poisson–Boltzmann theory, we confirm this conclusion and show that simple Donnan theory is accurate even for the smallest of viruses and virus-like particles. This, in part, is due to the additional screening caused by the presence of a large number of immobile charges in the cavity of the shell. The presence of a net charge on the outer surface of the capsid we find in practice to not have a large effect on the pH shift. Hence, Donnan theory can indeed be applied to connect the local pH and the amount of encapsulated material. The large shifts up to a full pH unit that we predict must have consequences for applications of virus capsids as nanocontainers in bionanotechnology and artificial cell organelles. American Chemical Society 2023-03-07 /pmc/articles/PMC10026070/ /pubmed/36881522 http://dx.doi.org/10.1021/acs.jpcb.2c08604 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Muhren, H. J. van der Schoot, Paul Electrostatic Theory of the Acidity of the Solution in the Lumina of Viruses and Virus-Like Particles |
title | Electrostatic Theory
of the Acidity of the Solution
in the Lumina of Viruses and Virus-Like Particles |
title_full | Electrostatic Theory
of the Acidity of the Solution
in the Lumina of Viruses and Virus-Like Particles |
title_fullStr | Electrostatic Theory
of the Acidity of the Solution
in the Lumina of Viruses and Virus-Like Particles |
title_full_unstemmed | Electrostatic Theory
of the Acidity of the Solution
in the Lumina of Viruses and Virus-Like Particles |
title_short | Electrostatic Theory
of the Acidity of the Solution
in the Lumina of Viruses and Virus-Like Particles |
title_sort | electrostatic theory
of the acidity of the solution
in the lumina of viruses and virus-like particles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026070/ https://www.ncbi.nlm.nih.gov/pubmed/36881522 http://dx.doi.org/10.1021/acs.jpcb.2c08604 |
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