Bioassay for total serum bioactivity of Atractylodes lancea

The study aimed to establish a bioassay for total bioactivity of Atractylodes lancea (AL) in human serum samples. Inhibition of bacterial growth (Staphylococcus aureus ATCC 25923) was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The calibration curve (0, 0.39, 0...

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Detalles Bibliográficos
Autores principales: Na-Bangchang, Kesara, Cheoymang, Anurak, Muhamad, Nadda, Kulma, Inthuon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2023
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026330/
https://www.ncbi.nlm.nih.gov/pubmed/36950462
http://dx.doi.org/10.4103/japtr.japtr_431_22
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author Na-Bangchang, Kesara
Cheoymang, Anurak
Muhamad, Nadda
Kulma, Inthuon
author_facet Na-Bangchang, Kesara
Cheoymang, Anurak
Muhamad, Nadda
Kulma, Inthuon
author_sort Na-Bangchang, Kesara
collection PubMed
description The study aimed to establish a bioassay for total bioactivity of Atractylodes lancea (AL) in human serum samples. Inhibition of bacterial growth (Staphylococcus aureus ATCC 25923) was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The calibration curve (0, 0.39, 0.78, 1.56, 3.13, 2.56, and 50 ng/μl) was linear with correlation coefficients >0.990. The limit of quantification (LOQ) was 1.66 mg/ml using 20-ml serum sample. The developed bioassay method meets the standard of the bioanalytical method for determination of serum bioactivity of AL.
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spelling pubmed-100263302023-03-21 Bioassay for total serum bioactivity of Atractylodes lancea Na-Bangchang, Kesara Cheoymang, Anurak Muhamad, Nadda Kulma, Inthuon J Adv Pharm Technol Res Original Article The study aimed to establish a bioassay for total bioactivity of Atractylodes lancea (AL) in human serum samples. Inhibition of bacterial growth (Staphylococcus aureus ATCC 25923) was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The calibration curve (0, 0.39, 0.78, 1.56, 3.13, 2.56, and 50 ng/μl) was linear with correlation coefficients >0.990. The limit of quantification (LOQ) was 1.66 mg/ml using 20-ml serum sample. The developed bioassay method meets the standard of the bioanalytical method for determination of serum bioactivity of AL. Wolters Kluwer - Medknow 2023 2023-01-20 /pmc/articles/PMC10026330/ /pubmed/36950462 http://dx.doi.org/10.4103/japtr.japtr_431_22 Text en Copyright: © 2023 Journal of Advanced Pharmaceutical Technology & Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Na-Bangchang, Kesara
Cheoymang, Anurak
Muhamad, Nadda
Kulma, Inthuon
Bioassay for total serum bioactivity of Atractylodes lancea
title Bioassay for total serum bioactivity of Atractylodes lancea
title_full Bioassay for total serum bioactivity of Atractylodes lancea
title_fullStr Bioassay for total serum bioactivity of Atractylodes lancea
title_full_unstemmed Bioassay for total serum bioactivity of Atractylodes lancea
title_short Bioassay for total serum bioactivity of Atractylodes lancea
title_sort bioassay for total serum bioactivity of atractylodes lancea
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026330/
https://www.ncbi.nlm.nih.gov/pubmed/36950462
http://dx.doi.org/10.4103/japtr.japtr_431_22
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AT muhamadnadda bioassayfortotalserumbioactivityofatractylodeslancea
AT kulmainthuon bioassayfortotalserumbioactivityofatractylodeslancea

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