Cargando…

Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress

BACKGROUND: The Polygonatum cyrtonema Hua rhizomes (also known as Rhizoma Polygonati, RP) are consumed for their health benefits. The main source of the RP is wild P. cyrtonema populations in the Hunan province of China. However, the soil Cadmium (Cd) content in Huanan is increasing, thus increasing...

Descripción completa

Detalles Bibliográficos
Autores principales: Song, Rong, Yan, Bei, Xie, Jin, Zhou, Li, Xu, Rui, Zhou, Jia Min, Ji, Xiong Hui, Yi, Zi Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026435/
https://www.ncbi.nlm.nih.gov/pubmed/36935490
http://dx.doi.org/10.1186/s12870-023-04162-6
_version_ 1784909539697491968
author Song, Rong
Yan, Bei
Xie, Jin
Zhou, Li
Xu, Rui
Zhou, Jia Min
Ji, Xiong Hui
Yi, Zi Li
author_facet Song, Rong
Yan, Bei
Xie, Jin
Zhou, Li
Xu, Rui
Zhou, Jia Min
Ji, Xiong Hui
Yi, Zi Li
author_sort Song, Rong
collection PubMed
description BACKGROUND: The Polygonatum cyrtonema Hua rhizomes (also known as Rhizoma Polygonati, RP) are consumed for their health benefits. The main source of the RP is wild P. cyrtonema populations in the Hunan province of China. However, the soil Cadmium (Cd) content in Huanan is increasing, thus increasing the risks of Cd accumulation in RP which may end up in the human food chain. To understand the mechanism of Cd accumulation and resistance in P. cyrtonema, we subjected P. cyrtonema plants to four levels of Cd stress [(D2) 1, (D3) 2, (D4) 4, and (D5) 8 mg/kg)] compared to (D1) 0.5 mg/kg. RESULTS: The increase in soil Cd content up to 4 mg/kg resulted in a significant increase in tissue (root hair, rhizome, stem, and leaf) Cd content. The increase in Cd concentration variably affected the antioxidant enzyme activities. We could identify 14,171 and 12,115 protein groups and peptides, respectively. There were 193, 227, 260, and 163 differentially expressed proteins (DEPs) in D2, D3, D4, and D5, respectively, compared to D1. The number of downregulated DEPs increased with an increase in Cd content up to 4 mg/kg. These downregulated proteins belonged to sugar biosynthesis, amino acid biosynthesis-related pathways, and secondary metabolism-related pathways. Our results indicate that Cd stress increases ROS generation, against which, different ROS scavenging proteins are upregulated in P. cyrtonema. Moreover, Cd stress affected the expression of lipid transport and assembly, glycolysis/gluconeogenesis, sugar biosynthesis, and ATP generation. CONCLUSION: These results suggest that an increase in soil Cd content may end up in Huangjing. Cadmium stress initiates expression changes in multiple pathways related to energy metabolism, sugar biosynthesis, and secondary metabolite biosynthesis. The proteins involved in these pathways are potential candidates for manipulation and development of Cd stress-tolerant genotypes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04162-6.
format Online
Article
Text
id pubmed-10026435
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-100264352023-03-21 Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress Song, Rong Yan, Bei Xie, Jin Zhou, Li Xu, Rui Zhou, Jia Min Ji, Xiong Hui Yi, Zi Li BMC Plant Biol Research BACKGROUND: The Polygonatum cyrtonema Hua rhizomes (also known as Rhizoma Polygonati, RP) are consumed for their health benefits. The main source of the RP is wild P. cyrtonema populations in the Hunan province of China. However, the soil Cadmium (Cd) content in Huanan is increasing, thus increasing the risks of Cd accumulation in RP which may end up in the human food chain. To understand the mechanism of Cd accumulation and resistance in P. cyrtonema, we subjected P. cyrtonema plants to four levels of Cd stress [(D2) 1, (D3) 2, (D4) 4, and (D5) 8 mg/kg)] compared to (D1) 0.5 mg/kg. RESULTS: The increase in soil Cd content up to 4 mg/kg resulted in a significant increase in tissue (root hair, rhizome, stem, and leaf) Cd content. The increase in Cd concentration variably affected the antioxidant enzyme activities. We could identify 14,171 and 12,115 protein groups and peptides, respectively. There were 193, 227, 260, and 163 differentially expressed proteins (DEPs) in D2, D3, D4, and D5, respectively, compared to D1. The number of downregulated DEPs increased with an increase in Cd content up to 4 mg/kg. These downregulated proteins belonged to sugar biosynthesis, amino acid biosynthesis-related pathways, and secondary metabolism-related pathways. Our results indicate that Cd stress increases ROS generation, against which, different ROS scavenging proteins are upregulated in P. cyrtonema. Moreover, Cd stress affected the expression of lipid transport and assembly, glycolysis/gluconeogenesis, sugar biosynthesis, and ATP generation. CONCLUSION: These results suggest that an increase in soil Cd content may end up in Huangjing. Cadmium stress initiates expression changes in multiple pathways related to energy metabolism, sugar biosynthesis, and secondary metabolite biosynthesis. The proteins involved in these pathways are potential candidates for manipulation and development of Cd stress-tolerant genotypes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04162-6. BioMed Central 2023-03-20 /pmc/articles/PMC10026435/ /pubmed/36935490 http://dx.doi.org/10.1186/s12870-023-04162-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Song, Rong
Yan, Bei
Xie, Jin
Zhou, Li
Xu, Rui
Zhou, Jia Min
Ji, Xiong Hui
Yi, Zi Li
Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress
title Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress
title_full Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress
title_fullStr Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress
title_full_unstemmed Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress
title_short Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress
title_sort comparative proteome profiles of polygonatum cyrtonema hua rhizomes (rhizoma ploygonati) in response to different levels of cadmium stress
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026435/
https://www.ncbi.nlm.nih.gov/pubmed/36935490
http://dx.doi.org/10.1186/s12870-023-04162-6
work_keys_str_mv AT songrong comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress
AT yanbei comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress
AT xiejin comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress
AT zhouli comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress
AT xurui comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress
AT zhoujiamin comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress
AT jixionghui comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress
AT yizili comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress