Cargando…

MicroLED biosensor with colloidal quantum dots and smartphone detection

A fluorescence sensor with the capability for spatially multiplexed measurements utilizing smartphone detection is presented. Bioconjugated quantum dots are used as the fluorescent tag and are excited using a blue-emitting microLED (µLED). The 1-dimensional GaN µLED array is butt-coupled to one edge...

Descripción completa

Detalles Bibliográficos
Autores principales: Bruce, Natalie, Farrell, Francesca, Xie, Enyuan, Scullion, Mark G., Haughey, Anne-Marie, Gu, Erdan, Dawson, Martin D., Laurand, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optica Publishing Group 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026578/
https://www.ncbi.nlm.nih.gov/pubmed/36950244
http://dx.doi.org/10.1364/BOE.478276
_version_ 1784909566797938688
author Bruce, Natalie
Farrell, Francesca
Xie, Enyuan
Scullion, Mark G.
Haughey, Anne-Marie
Gu, Erdan
Dawson, Martin D.
Laurand, Nicolas
author_facet Bruce, Natalie
Farrell, Francesca
Xie, Enyuan
Scullion, Mark G.
Haughey, Anne-Marie
Gu, Erdan
Dawson, Martin D.
Laurand, Nicolas
author_sort Bruce, Natalie
collection PubMed
description A fluorescence sensor with the capability for spatially multiplexed measurements utilizing smartphone detection is presented. Bioconjugated quantum dots are used as the fluorescent tag and are excited using a blue-emitting microLED (µLED). The 1-dimensional GaN µLED array is butt-coupled to one edge of the glass slide to take advantage of total internal reflection fluorescence (TIRF) principles. The bioassays on the top surface of the glass waveguide are excited and the resultant fluorescence is detected with the smartphone. The red, green, and blue channels of the digital image are utilized to spectrally separate the excitation light from the fluorescence for analysis. Using a biotin-functionalized glass slide as proof of principle, we have shown that streptavidin conjugated quantum dots can be detected down to a concentration of 8 nM.
format Online
Article
Text
id pubmed-10026578
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Optica Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-100265782023-03-21 MicroLED biosensor with colloidal quantum dots and smartphone detection Bruce, Natalie Farrell, Francesca Xie, Enyuan Scullion, Mark G. Haughey, Anne-Marie Gu, Erdan Dawson, Martin D. Laurand, Nicolas Biomed Opt Express Article A fluorescence sensor with the capability for spatially multiplexed measurements utilizing smartphone detection is presented. Bioconjugated quantum dots are used as the fluorescent tag and are excited using a blue-emitting microLED (µLED). The 1-dimensional GaN µLED array is butt-coupled to one edge of the glass slide to take advantage of total internal reflection fluorescence (TIRF) principles. The bioassays on the top surface of the glass waveguide are excited and the resultant fluorescence is detected with the smartphone. The red, green, and blue channels of the digital image are utilized to spectrally separate the excitation light from the fluorescence for analysis. Using a biotin-functionalized glass slide as proof of principle, we have shown that streptavidin conjugated quantum dots can be detected down to a concentration of 8 nM. Optica Publishing Group 2023-02-10 /pmc/articles/PMC10026578/ /pubmed/36950244 http://dx.doi.org/10.1364/BOE.478276 Text en Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Bruce, Natalie
Farrell, Francesca
Xie, Enyuan
Scullion, Mark G.
Haughey, Anne-Marie
Gu, Erdan
Dawson, Martin D.
Laurand, Nicolas
MicroLED biosensor with colloidal quantum dots and smartphone detection
title MicroLED biosensor with colloidal quantum dots and smartphone detection
title_full MicroLED biosensor with colloidal quantum dots and smartphone detection
title_fullStr MicroLED biosensor with colloidal quantum dots and smartphone detection
title_full_unstemmed MicroLED biosensor with colloidal quantum dots and smartphone detection
title_short MicroLED biosensor with colloidal quantum dots and smartphone detection
title_sort microled biosensor with colloidal quantum dots and smartphone detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026578/
https://www.ncbi.nlm.nih.gov/pubmed/36950244
http://dx.doi.org/10.1364/BOE.478276
work_keys_str_mv AT brucenatalie microledbiosensorwithcolloidalquantumdotsandsmartphonedetection
AT farrellfrancesca microledbiosensorwithcolloidalquantumdotsandsmartphonedetection
AT xieenyuan microledbiosensorwithcolloidalquantumdotsandsmartphonedetection
AT scullionmarkg microledbiosensorwithcolloidalquantumdotsandsmartphonedetection
AT haugheyannemarie microledbiosensorwithcolloidalquantumdotsandsmartphonedetection
AT guerdan microledbiosensorwithcolloidalquantumdotsandsmartphonedetection
AT dawsonmartind microledbiosensorwithcolloidalquantumdotsandsmartphonedetection
AT laurandnicolas microledbiosensorwithcolloidalquantumdotsandsmartphonedetection