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MicroLED biosensor with colloidal quantum dots and smartphone detection
A fluorescence sensor with the capability for spatially multiplexed measurements utilizing smartphone detection is presented. Bioconjugated quantum dots are used as the fluorescent tag and are excited using a blue-emitting microLED (µLED). The 1-dimensional GaN µLED array is butt-coupled to one edge...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optica Publishing Group
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026578/ https://www.ncbi.nlm.nih.gov/pubmed/36950244 http://dx.doi.org/10.1364/BOE.478276 |
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author | Bruce, Natalie Farrell, Francesca Xie, Enyuan Scullion, Mark G. Haughey, Anne-Marie Gu, Erdan Dawson, Martin D. Laurand, Nicolas |
author_facet | Bruce, Natalie Farrell, Francesca Xie, Enyuan Scullion, Mark G. Haughey, Anne-Marie Gu, Erdan Dawson, Martin D. Laurand, Nicolas |
author_sort | Bruce, Natalie |
collection | PubMed |
description | A fluorescence sensor with the capability for spatially multiplexed measurements utilizing smartphone detection is presented. Bioconjugated quantum dots are used as the fluorescent tag and are excited using a blue-emitting microLED (µLED). The 1-dimensional GaN µLED array is butt-coupled to one edge of the glass slide to take advantage of total internal reflection fluorescence (TIRF) principles. The bioassays on the top surface of the glass waveguide are excited and the resultant fluorescence is detected with the smartphone. The red, green, and blue channels of the digital image are utilized to spectrally separate the excitation light from the fluorescence for analysis. Using a biotin-functionalized glass slide as proof of principle, we have shown that streptavidin conjugated quantum dots can be detected down to a concentration of 8 nM. |
format | Online Article Text |
id | pubmed-10026578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Optica Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-100265782023-03-21 MicroLED biosensor with colloidal quantum dots and smartphone detection Bruce, Natalie Farrell, Francesca Xie, Enyuan Scullion, Mark G. Haughey, Anne-Marie Gu, Erdan Dawson, Martin D. Laurand, Nicolas Biomed Opt Express Article A fluorescence sensor with the capability for spatially multiplexed measurements utilizing smartphone detection is presented. Bioconjugated quantum dots are used as the fluorescent tag and are excited using a blue-emitting microLED (µLED). The 1-dimensional GaN µLED array is butt-coupled to one edge of the glass slide to take advantage of total internal reflection fluorescence (TIRF) principles. The bioassays on the top surface of the glass waveguide are excited and the resultant fluorescence is detected with the smartphone. The red, green, and blue channels of the digital image are utilized to spectrally separate the excitation light from the fluorescence for analysis. Using a biotin-functionalized glass slide as proof of principle, we have shown that streptavidin conjugated quantum dots can be detected down to a concentration of 8 nM. Optica Publishing Group 2023-02-10 /pmc/articles/PMC10026578/ /pubmed/36950244 http://dx.doi.org/10.1364/BOE.478276 Text en Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Bruce, Natalie Farrell, Francesca Xie, Enyuan Scullion, Mark G. Haughey, Anne-Marie Gu, Erdan Dawson, Martin D. Laurand, Nicolas MicroLED biosensor with colloidal quantum dots and smartphone detection |
title | MicroLED biosensor with colloidal quantum dots and smartphone detection |
title_full | MicroLED biosensor with colloidal quantum dots and smartphone detection |
title_fullStr | MicroLED biosensor with colloidal quantum dots and smartphone detection |
title_full_unstemmed | MicroLED biosensor with colloidal quantum dots and smartphone detection |
title_short | MicroLED biosensor with colloidal quantum dots and smartphone detection |
title_sort | microled biosensor with colloidal quantum dots and smartphone detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026578/ https://www.ncbi.nlm.nih.gov/pubmed/36950244 http://dx.doi.org/10.1364/BOE.478276 |
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