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Enhancing Mucosal-Associated Invariant T Cell Function and Expansion with Human Selective Serum
Mucosal-associated invariant T (MAIT) cells are promising innate-like lymphocytes with potential for use in anti-tumor immunotherapy. Existing MAIT cell expansion protocols are associated with potentially decremental phenotypic changes, including increased frequency of CD4(+) MAIT cells and higher i...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AAI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026854/ https://www.ncbi.nlm.nih.gov/pubmed/36651819 http://dx.doi.org/10.4049/immunohorizons.2200082 |
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author | Labuz, Daniel Cacioppo, Jackson Li, Kelin Aubé, Jeffrey Leung, Daniel T. |
author_facet | Labuz, Daniel Cacioppo, Jackson Li, Kelin Aubé, Jeffrey Leung, Daniel T. |
author_sort | Labuz, Daniel |
collection | PubMed |
description | Mucosal-associated invariant T (MAIT) cells are promising innate-like lymphocytes with potential for use in anti-tumor immunotherapy. Existing MAIT cell expansion protocols are associated with potentially decremental phenotypic changes, including increased frequency of CD4(+) MAIT cells and higher inhibitory receptor expression. In this study, we compared the effect on expansion of human MAIT cells of a serum replacement, Physiologix XF SR (Phx), with traditional serum FBS for supplementing RPMI 1640 media. Using flow cytometry, we found that Phx supported a significantly higher proliferative capacity for MAIT cells and resulted in a lower frequency of CD4(+) MAIT cells, which have been associated with reduced Th1 effector and cytolytic functions. We saw that culturing MAIT cells in Phx led to better survival of MAIT cells and lower frequency of PD-1(+) MAIT cells than FBS-supplemented media. Functionally, we saw that Phx supplementation was associated with a higher frequency of IFN-γ(+) MAIT cells after stimulation with Escherichia coli than FBS-supplemented RPMI. In conclusion, we show that MAIT cells cultured in Phx have higher proliferative capacity, lower expression of inhibitory receptors, and higher capacity to produce IFN-γ after E. coli stimulation than FBS-supplemented RPMI. This work shows that expanding MAIT cells with Phx compared with FBS-supplemented RPMI results in a more functionally desirable MAIT cell for future anti-tumor immunotherapy. |
format | Online Article Text |
id | pubmed-10026854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | AAI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100268542023-03-20 Enhancing Mucosal-Associated Invariant T Cell Function and Expansion with Human Selective Serum Labuz, Daniel Cacioppo, Jackson Li, Kelin Aubé, Jeffrey Leung, Daniel T. Immunohorizons Clinical and Translational Immunology Mucosal-associated invariant T (MAIT) cells are promising innate-like lymphocytes with potential for use in anti-tumor immunotherapy. Existing MAIT cell expansion protocols are associated with potentially decremental phenotypic changes, including increased frequency of CD4(+) MAIT cells and higher inhibitory receptor expression. In this study, we compared the effect on expansion of human MAIT cells of a serum replacement, Physiologix XF SR (Phx), with traditional serum FBS for supplementing RPMI 1640 media. Using flow cytometry, we found that Phx supported a significantly higher proliferative capacity for MAIT cells and resulted in a lower frequency of CD4(+) MAIT cells, which have been associated with reduced Th1 effector and cytolytic functions. We saw that culturing MAIT cells in Phx led to better survival of MAIT cells and lower frequency of PD-1(+) MAIT cells than FBS-supplemented media. Functionally, we saw that Phx supplementation was associated with a higher frequency of IFN-γ(+) MAIT cells after stimulation with Escherichia coli than FBS-supplemented RPMI. In conclusion, we show that MAIT cells cultured in Phx have higher proliferative capacity, lower expression of inhibitory receptors, and higher capacity to produce IFN-γ after E. coli stimulation than FBS-supplemented RPMI. This work shows that expanding MAIT cells with Phx compared with FBS-supplemented RPMI results in a more functionally desirable MAIT cell for future anti-tumor immunotherapy. AAI 2023-01-18 /pmc/articles/PMC10026854/ /pubmed/36651819 http://dx.doi.org/10.4049/immunohorizons.2200082 Text en Copyright © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under the terms of the CC BY-NC-ND 4.0 Unported license (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Clinical and Translational Immunology Labuz, Daniel Cacioppo, Jackson Li, Kelin Aubé, Jeffrey Leung, Daniel T. Enhancing Mucosal-Associated Invariant T Cell Function and Expansion with Human Selective Serum |
title | Enhancing Mucosal-Associated Invariant T Cell Function and Expansion with Human Selective Serum |
title_full | Enhancing Mucosal-Associated Invariant T Cell Function and Expansion with Human Selective Serum |
title_fullStr | Enhancing Mucosal-Associated Invariant T Cell Function and Expansion with Human Selective Serum |
title_full_unstemmed | Enhancing Mucosal-Associated Invariant T Cell Function and Expansion with Human Selective Serum |
title_short | Enhancing Mucosal-Associated Invariant T Cell Function and Expansion with Human Selective Serum |
title_sort | enhancing mucosal-associated invariant t cell function and expansion with human selective serum |
topic | Clinical and Translational Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026854/ https://www.ncbi.nlm.nih.gov/pubmed/36651819 http://dx.doi.org/10.4049/immunohorizons.2200082 |
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