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A trimeric spike-based COVID-19 vaccine candidate induces broad neutralization against SARS-CoV-2 variants
COVID-19 pandemic caused by SARS-CoV-2 infection has an impact on global public health and social economy. The emerging immune escape of SARS-CoV-2 variants pose great challenges to the development of vaccines based on original strains. The development of second-generation COVID-19 vaccines to induc...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026892/ https://www.ncbi.nlm.nih.gov/pubmed/36882925 http://dx.doi.org/10.1080/21645515.2023.2186110 |
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author | Gao, Feixia Zheng, Mei Fan, Jiangfeng Ding, Yahong Liu, Xueying Zhang, Min Zhang, Xin Dong, Jinrong Zhou, Xu Luo, Jian Li, Xiuling |
author_facet | Gao, Feixia Zheng, Mei Fan, Jiangfeng Ding, Yahong Liu, Xueying Zhang, Min Zhang, Xin Dong, Jinrong Zhou, Xu Luo, Jian Li, Xiuling |
author_sort | Gao, Feixia |
collection | PubMed |
description | COVID-19 pandemic caused by SARS-CoV-2 infection has an impact on global public health and social economy. The emerging immune escape of SARS-CoV-2 variants pose great challenges to the development of vaccines based on original strains. The development of second-generation COVID-19 vaccines to induce immune responses with broad-spectrum protective effects is a matter of great urgency. Here, a prefusion-stabilized spike (S) trimer protein based on B.1.351 variant was expressed and prepared with CpG7909/aluminum hydroxide dual adjuvant to investigate the immunogenicity in mice. The results showed that the candidate vaccine could induce a significant receptor binding domain-specific antibody response and a substantial interferon-γ-mediated immune response. Furthermore, the candidate vaccine also elicited robust cross-neutralization against the pseudoviruses of the original strain, Beta variant, Delta variant and Omicron variant. The vaccine strategy of S-trimer protein formulated with CpG7909/aluminum hydroxide dual adjuvant may be considered a means to increase vaccine effectiveness against future variants. |
format | Online Article Text |
id | pubmed-10026892 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-100268922023-03-21 A trimeric spike-based COVID-19 vaccine candidate induces broad neutralization against SARS-CoV-2 variants Gao, Feixia Zheng, Mei Fan, Jiangfeng Ding, Yahong Liu, Xueying Zhang, Min Zhang, Xin Dong, Jinrong Zhou, Xu Luo, Jian Li, Xiuling Hum Vaccin Immunother Coronavirus COVID-19 pandemic caused by SARS-CoV-2 infection has an impact on global public health and social economy. The emerging immune escape of SARS-CoV-2 variants pose great challenges to the development of vaccines based on original strains. The development of second-generation COVID-19 vaccines to induce immune responses with broad-spectrum protective effects is a matter of great urgency. Here, a prefusion-stabilized spike (S) trimer protein based on B.1.351 variant was expressed and prepared with CpG7909/aluminum hydroxide dual adjuvant to investigate the immunogenicity in mice. The results showed that the candidate vaccine could induce a significant receptor binding domain-specific antibody response and a substantial interferon-γ-mediated immune response. Furthermore, the candidate vaccine also elicited robust cross-neutralization against the pseudoviruses of the original strain, Beta variant, Delta variant and Omicron variant. The vaccine strategy of S-trimer protein formulated with CpG7909/aluminum hydroxide dual adjuvant may be considered a means to increase vaccine effectiveness against future variants. Taylor & Francis 2023-03-07 /pmc/articles/PMC10026892/ /pubmed/36882925 http://dx.doi.org/10.1080/21645515.2023.2186110 Text en © 2023 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way. |
spellingShingle | Coronavirus Gao, Feixia Zheng, Mei Fan, Jiangfeng Ding, Yahong Liu, Xueying Zhang, Min Zhang, Xin Dong, Jinrong Zhou, Xu Luo, Jian Li, Xiuling A trimeric spike-based COVID-19 vaccine candidate induces broad neutralization against SARS-CoV-2 variants |
title | A trimeric spike-based COVID-19 vaccine candidate induces broad neutralization against SARS-CoV-2 variants |
title_full | A trimeric spike-based COVID-19 vaccine candidate induces broad neutralization against SARS-CoV-2 variants |
title_fullStr | A trimeric spike-based COVID-19 vaccine candidate induces broad neutralization against SARS-CoV-2 variants |
title_full_unstemmed | A trimeric spike-based COVID-19 vaccine candidate induces broad neutralization against SARS-CoV-2 variants |
title_short | A trimeric spike-based COVID-19 vaccine candidate induces broad neutralization against SARS-CoV-2 variants |
title_sort | trimeric spike-based covid-19 vaccine candidate induces broad neutralization against sars-cov-2 variants |
topic | Coronavirus |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026892/ https://www.ncbi.nlm.nih.gov/pubmed/36882925 http://dx.doi.org/10.1080/21645515.2023.2186110 |
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